Binding of Pb(II) in the system humic acid/goethite at acidic pH

The study of Pb(II) binding to the system humic acid/goethite in acidic medium is reported in the present paper. From a macroscopic point of view, we have constructed the experimental sorption isotherms (using atomic absorption spectroscopy) and compared them with the prediction of the additivity rule. It is found that this system presents positive deviations, that is, the amount sorbed is about an order of magnitude higher than predicted. Apart from this, microscopic and structural aspects have also been studied using in situ and ex situ infrared spectroscopy. These results suggest that the presence of Pb(II) increases the amount of humic acid bound to the oxide. It is proposed that proton displacement due to the interaction between humic substances and the oxide, along with the formation of ternary complexes with the Pb(II) cation bridging the oxide and the humic substances (Type A complexes), cause the deviation from additivity.     

Enantiomeric fractions and congener specific determination of polychlorinated biphenyls in eggs of predatory birds from Doñana National Park (Spain)

The content of 30 polychlorinated biphenyls (PCBs) and the enantiomeric fractions of 10 chiral PCBs were determined in 17 infertile eggs from three different predatory bird species collected in Do?ana National Park (DNP, Spain) in the period 1999-2000. The highest PCB concentration was found in eggs from red kites (0.52-110 microg/g on a fresh weight basis, f.w.) followed by buzzard (0.08-13 microg/g f.w.) and booted eagle (0.10-1.5 microg/g f.w.). Seventy-five percent of the red kite eggs had PCB levels above 4.7 microg/g f.w., which is associated in the literature with reproductive failure. This could be related to the fact that red kite populations have decreased by more than 50% in the last five years in DNP. PCBs # 138, 153, and 180 were the most abundant in all cases. This is the first time that atropisomers of 10 chiral PCBs (PCBs # 45, 84, 91, 95, 132, 135, 136, 149, 174, and 176) in predatory bird eggs have been performed. The enantiomeric fractions (EFs) for most PCBs investigated were non-racemic (EF not = 0.5), ranging from 0.05 to 0.95. The results suggested that predatory birds, mainly red kite species, are highly polluted by PCBs, and PCBs # 95, 132, 135, 136, and 174 strongly deviate from the racemic-mixture values.     

Reductive dehalogenation of tetrabromobisphenol-A by sediment from a contaminated ephemeral streambed and an enrichment culture

This study was aimed at improving our understanding of the physiology of the microorganisms that reductively dehalogenate tetrabromobisphenol-A (TBBPA). Activity was followed in contaminated sediments from a polluted streambed as well as from fracture filling material underlying the stream. Reductive dehalogenation was observed in surface sediments but not in fracture filling samples from a depth of 3m. Likewise, anaerobic microbial activity, represented by sulphate reduction, was much higher in the surface sediment. In the culture enriched from the surface sediment, optimal microbial debromination of TBBPA took place at a salinity of 2% and 3% NaCl, temperature of 30 degrees Celsius, and pH of 7-8. Ethanol, pyruvate and the combination of hydrogen with acetate were the most suitable electron donors and carbon sources for this culture. Alternative electron acceptors like Fe(3+), SO(4)(2-), SO(3)(2-), NO(3)(-) and 2,4,6-tribromophenol inhibited TBBPA debromination. The debrominating bacteria were heat sensitive (80 degrees Celsius, for 10min) but were not inhibited by bromoethanesulphonate or molybdate. This study allowed optimisation of our culturing conditions, but was also important for understanding the factors which influence TBBPA debromination in situ.     

Environmental Impact Assessment of Polylactide(PLA)/Chicken Feathers Biocomposite Materials

The aim of this study is to analyse the environmental impacts (EIs) of the process of preparation of new biocomposite materials obtained from polylactide (PLA) and chicken feathers (CFs). Two CFs stabilization methods and different percentages of CFs have been studied. The EIs of these new composites were compared to the impact of virgin PLA. Cradle-to-gate life cycle inventories were assessed for 0–35% v/v of CFs in a CFs/PLA biocomposite. Two CFs stabilization processes, autoclave and surfactant, were tested and compared with the aim to prioritize one of them from the environmental point of view. A composite plate of 184?×?184?×?2.2 mm³ was defined as the functional unit. Autoclave stabilization process exhibited lower environmental impact compared with surfactant stabilization process mainly due to both the lower requirements of electricity and water and the reduced pollution loads of the generated wastewater. Thus, the autoclave process was selected as the standard method when comparing the EIs of the proposed CFs/PLA biocomposites. In this sense, the addition of CFs to PLA matrix proportionally reduces all the EIs compared to pure PLA due to the replacement of PLA with CFs. This behaviour can be explained because the PLA production accounts for the 99% of the impact of the biocomposite. Consequently, CFs conveniently stabilized might be an alternative raw material to prepare CFs/PLA biocomposites with less environmental impact compared to pure PLA.     

Meta-analysis of the differential effects of habitat fragmentation and degradation on plant genetic diversity

Genetic diversity is a key factor for population survival and evolution. However, anthropogenic habitat disturbance can erode it, making populations more prone to extinction. Aiming to assess the global effects of habitat disturbance on plant genetic variation, we conducted a meta-analysis based on 92 case studies obtained from published literature. We compared the effects of habitat fragmentation and degradation on plant allelic richness and gene diversity (equivalent to expected heterozygosity) and tested whether such changes are sensitive to different life-forms, life spans, mating systems, and commonness. Anthropogenic disturbance had a negative effect on allelic richness, but not on gene diversity. Habitat fragmentation had a negative effect on genetic variation, whereas habitat degradation had no effect. When we examined the individual effects in fragmented habitats, allelic richness and gene diversity decreased, but this decrease was strongly dependent on certain plant traits. Specifically, common long-lived trees and self-incompatible species were more susceptible to allelic richness loss. Conversely, gene diversity decreased in common short-lived species (herbs) with self-compatible reproduction. In a wider geographical context, tropical plant communities were more sensitive to allelic richness loss, whereas temperate plant communities were more sensitive to gene diversity loss. Our synthesis showed complex responses to habitat disturbance among plant species. In many cases, the absence of effects could be the result of the time elapsed since the disturbance event or reproductive systems favoring self-pollination, but attention must be paid to those plant species that are more susceptible to losing genetic diversity, and appropriate conservation should be actions taken.     

Effect of the veterinary ionophore monensin on the structure and activity of a tropical soil bacterial community

Abstract

Monensin (MON) is a coccidiostat used as a growth promoter that can reach the environment through fertilization with manure from farm animals. To verify whether field-relevant concentrations of this drug negatively influence the structure and activity of tropical soil bacteria, plate counts, CO₂ efflux measurements, phospholipid fatty acids (PLFA) and community-level physiological profiling (CLPP) profiles were obtained for soil microcosms exposed to 1 or 10?mg kg^?1 of MON across 11?days. Although 53% (1?mg kg^?1) to 40% (10?mg kg^?1) of the MON concentrations added to the microcosms dissipated within 5?days, a subtle concentration-dependent decrease in the number of culturable bacteria (<1 log CFU g^?1), reduced (?20 to ?30%) or exacerbated (+25%) soil CO₂ effluxes, a marked shift of non-bacterial fatty acids, and altered respiration of amines (1.22-fold decrease) and polymers (1.70-fold increase) were noted in some of the treatments. These results suggest that MON quickly killed some microorganisms and that the surviving populations were selected and metabolically stimulated. Consequently, MON should be monitored in agronomic and environmental systems as part of One Health efforts.     

Water Remediation: PVA-Based Magnetic Gels as Efficient Devices to Heavy Metal Removal

Scientific and technological researches are devoted to obtain materials capable of retaining different kinds of pollutants, contributing to contamination solutions. In this context, hydrogels have emerged as great candidates because of their excellent absorption properties as well as good mechanical, thermal and chemical properties. More specifically, ferrogels (magnetic gels) present the extra advantage of being easily manipulated by a permanent magnet. Here, we present the results derived from the application of ferrogels as efficient tools to extract heavy metal pollutants from wastewater samples. The gels were prepared following the method of freezing and thawing of a polyvinyl alcohol aqueous solution with magnetic nanoparticles coated with polyacrylic acid. Ferrogels were fully characterized and their ability to retain Cu²⁺ and Cd²⁺, as model heavy metals, was studied. Thus kinetics and mechanisms of adsorption were evaluated and modeled. The concentration of MNPs on the PVA matrix was key to improve the adsorption capability (approximately the double of retention is improved by the MNPs addition). The adsorption kinetics was determined as pseudo-second order model, whereas the Langmuir model was the most appropriate to explain the behavior of the gels. Finally reuse ability was evaluated to determine the real potential of these materials, the ferrogels demonstrated high efficiency up to about five cycles, retaining about 80–90% of their initial adsorption capability. All the results indicated that the materials are promising candidates able to compete with the commercial technology regarding to water remediation.     

Dynamics of a peculiar plant-herbivore relationship: the photosynthetic ascoglossan Elysia timida and the chlorophycean Acetabularia acetabulum

The ascoglossan mollusc Elysia timida Risso, 1818 retains functional chloroplasts from its algal food, the chlorophycean Acetabularia acetabulum (L.). Photosynthates from the plastids are an important source of organic nutrients for the mollusc. Chloroplast exploitation has an ecological function, allowing the ascoglossan to live entirely on an algal diet which is of limited, seasonal availability to other herbivores. Between October 1987 and July 1988, the annual evolution of the molluscan and algal populations was studied in a cove of Mazarrón Bay, southeast Spain. The population density of the mollusc is highly dependent on its food supply, being controlled by the seasonal life cycle of the algal population. During its life cycle, the degree of grazing by the mollusc decreases with increasing algal calcification, the cell walls of the alga progressively calcify, and the eventually highly calcificied stalks are completely resistant to ascoglossan grazing. In contrast, the exploitation of the algal chloroplasts retained by the molluscs increases during the seasonal cycle. The progressively increasing scarcity of food during the seasonal cycle may have led to the retention of symbiotic chloroplasts by E. timida. The developmental strategy of the ascoglossan also changes during the year: when food is abundant (in November, December, January, February and March) it is direct, with no planktonic larval phase, when food is scarce (in October, April, May and June) it is lecithotrophic, with a short planktonic larval phase. Chloroplast retention acts as a buffer, alleviating the effects of annual changes in density, structure and abundance of the alga on the nutritional state of the molluse.