Two Pilot Plant Reactors Designed for the In Situ Bioremediation of Chlorobenzene-contaminated Ground Water: Hydrogeological and Chemical Characteristics and Bacterial Consortia

The SAFIRA in situ pilot plant in Bitterfeld, Saxonia-Anhalt, Germany, currently serves as the test site for eight different in situ approaches to remediate anoxic chlorobenzene (CB)-contaminated ground water. Two reactors, both filled with originallignite-containing aquifer material, are designed for themicrobiological in situ remediation of the ground water bythe indigenous microbial consortia. In this study, thehydrogeological, chemical and microbiological conditions of theinflowing ground water and reactor filling material are presented,in order to establish the scientific basis for the start of thebioremediation process itself. The reactors were put intooperation in June 1999. In the following, inflow CB concentrationsin the ground water varied between 22 and 33 mg L^-1; achemical steady state for CB in both reactors was reachedafter 210 till 260 days operation time. The sediments werecolonized by high numbers of aerobic, iron-reducing anddenitrifying bacteria, as determined after 244 and 285 days ofoperation time. Furthermore, aerobic CB-degrading bacteria weredetected in all reactor zones. Comparative sequence analysis of16S rDNA gene clone libraries suggest the dominance of Proteobacteria (Comamonadaceae, Alcaligenaceae, Gallionella group, Acidithiobacillus) and members of theclass of low G+C gram-positive bacteria in the reactorsediments. In the inflowing ground water, sequences withphylogenetic affiliation to sulfate-reducing bacteria andsequences not affiliated with the known phyla of Bacteria, were found.     

Vertical distribution and feeding patterns of midwater fish in the central equatorial Atlantic

Fishes and zooplankton were obtained (March–April 1979 and partly in August 1974) from 45 hauls taken during the day and at night in the central equatorial Atlantic between Latitude 3°N and 2°S from the surface to 1250-m depth, using the RMT 1+8, a combined opening-closing plankton and micronekton trawl. The vertical distribution of 30 myctophid species is described. All species migrate in a diel pattern, Ceratoscopelus warmingii and Lampanyctus photonotus down to at least 1250 m. During daytime most species aggregated at 400-to 700-m depth, therefore only partly occupying the depth of the Deep Scattering Layer (400 to 500 m at 15 kHz). The feeding patterns of seven of the most abundant species were compared, with a total of 1 905 stomach contents being analysed. All seven species are regarded as opportunistic predators, which feed predominantly during the night on calanoid copepods. A total of 66 species of calanoid copepods were identified among the prey items, with smaller species definitely being in the minority. Stomachs of C. warmingii (700 to 1 250 m depth) and Lepidophanes guentheri (500 to 900 m depth) from daytime samples contained copepod species restricted to the upper 150 m of the water column, including Undinula vulgaris, Nannocalanus minor, and Euchaeta marina, thereby confirming an extended vertical migration of predators. Differences in diet and preferences between species in their total food spectrum are described.     

Postgraduale Weiterbildung, Nachwuchsf?rderpreis 2005, Jahrestagung 2006 in Landau

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