Muscle tetanus and loading condition effects on the elastic and viscous characteristics of the thorax

Thoracic deformation under an applied load is an established indicator of injury risk, but the force required to achieve an injurious level of deformation currently is not understood adequately. This article evaluates how two potentially important factors, loading condition and muscle tensing, affect the structural response of the dynamically loaded thorax. Structural models of two human cadaver thoraxes and two porcine thoraxes were used to quantify the effects. The human cadavers, which represent anthropometric extremes, were subjected to anterior loading from (1) a 5.1-cm-wide belt oriented diagonally (i.e., seatbelt-like loading), (2) a 15.2-cm-diameter rigid hub, and (3) a 20.3-cm-wide belt oriented laterally (i.e., a distributed load). A structural model having the mathematical formulation of a quasilinear viscoelastic material model was used to model the elastic and viscous response, with ramp-hold tests used to determine the model coefficients. The effect of thoracic musculature was assessed using similar ramp-hold tests on the porcine subjects, each with and without forced muscle contraction. Even maximally contracted thoracic musculature is shown to have a minimal effect on the response, with similar elastic and viscous characteristics exhibited by each subject regardless of muscle tone. The elastic response is shown to be approximately a factor of three stiffer for diagonal belt loading and for this distributed loading condition than for the hub loading, indicating that the response is influenced most by the particular anatomical structures that are engaged and, secondarily, by the area of load application. Specifically, shoulder involvement is shown to have a strong influence. The force relaxation is found to be pronounced, but insensitive to the loading condition, with long-time force relaxation coefficients (G( infinity )) in the range of 0.1 to 0.3. The findings of this study provide restraint-specific guidelines for the force-deflection characteristics of both physical and computational thoracic models.     

MaryPODS revisited: updated crash analysis and implications for screening program implementation

PROBLEM: Due to the relative scarcity of crashes, there has consistently been a problem with analyses that use crashes as a criterion measure in their analyses. METHOD: Previous analyses of the relationships between functional capacity measures and at-fault crash involvement for older drivers as reported in the NHTSA Model Driver Screening and Evaluation Program Final Technical Report have been updated to include one additional year of driving experience. Eighteen new at-fault crashes involving drivers who previously had no crash involvement were recorded for the Maryland Motor Vehicle Administration (MVA) test sample during this interval. The method of odds ratio (OR) calculation was used to examine the relationships between functional status predictors and the most salient among the safety outcome measures identified in the Maryland research. Peak valid OR values for the prior and current analyses were contrasted, and the stability of candidate pass-fail cut-points for each predictor relative to values identified in the Final Technical Report was examined. RESULTS: Results indicate that the predictive value of functional tests appears to decrease over time, particularly for the perceptual-cognitive measures. IMPACT ON INDUSTRY: The impact of these findings on programs and policies is to underscore a need for periodic reevaluation, spaced at the shortest practical intervals but not more than 2 years apart, in order for functional capacity screening to be applied effectively by licensing authorities, health care professionals, and others to reduce personal risk and enhance public safety.     

Targeted sampling protocol as prelude to bacterial source tracking with Enterococcus faecalis

Recent studies suggest that host origin databases for bacterial source tracking (BST) must contain a large number of isolates because bacterial subspecies change with geography and time. A new targeted sampling protocol was developed as a prelude to BST to minimize these changes. The research was conducted on the Sapelo River, a tidal river on the Georgia coast. A general sampling of the river showed fecal enterococcal numbers ranging from <10 (below the limit of detection) to 990 colony-forming units (CFU) per 100 mL. Locations with high enterococcal numbers were combined with local knowledge to determine targeted sampling sites. Fecal enterococcal numbers around one site ranged from <10 to 24,000 CFU per 100 mL. Bacterial source tracking was conducted to determine if a wastewater treatment facility at the site was responsible for this contamination. The fecal indicator bacterium was Enterococcus faecalis. Ribotyping, automated with a RiboPrinter (DuPont Qualicon, Wilmington, DE), was the BST method. Thirty-seven ribotypes were observed among 83 Ent. faecalis isolates obtained from the Sapelo River and the wastewater lagoon. Sixteen ribotypes were associated with either the river or the lagoon, and only five ribotypes (14%) were shared. Nevertheless, these five ribotypes represented 39 of the 83 Ent. faecalis isolates, almost a majority (47%). These results suggest that the fecal contamination in the river came from the wastewater treatment facility. As a prelude to BST, targeted sampling minimized subspecies changes with geography and time, and eliminated the need for a permanent host origin database by restricting BST to a small geographic area and requiring sampling to be completed in one day.     

Earthworm additions affect leachate production and nitrogen losses in typical midwestern agroecosystems

Earthworms affect soil structure and the movement of agrochemicals. Yet, there have been few field-scale studies that quantify the effect of earthworms on dissolved nitrogen fluxes in agroecosystems. We investigated the influence of semi-annual earthworm additions on leachate production and quality in different row crop agroecosystems. Chisel-till corn (Zea mays L.)-soybean [Glycine max (L.) Merr.] rotation (CT) and ridge-till corn-soybean-wheat (Triticum aestivum L.) rotation (RT) plots were arranged in a complete randomized block design (n = 3) with earthworm treatments (addition and ambient) as subplots where zero-tension lysimeters were placed 45 cm below ground. We assessed earthworm populations semi-annually and collected leachate biweekly over a three-year period and determined leachate volume and concentrations of total inorganic nitrogen (TIN) and dissolved organic nitrogen (DON). Abundance of deep-burrowing earthworms was increased in addition treatments over ambient and for both agroecosystems. Leachate loss was similar among agroecosystems, but earthworm additions increased leachate production in the range of 4.5 to 45.2% above ambient in CT cropping. Although leachate TIN and DON concentrations were generally similar between agroecosystems or earthworm treatments, transport of TIN was significantly increased in addition treatments over ambient in CT cropping due to increased leachate volume. Losses of total nitrogen in leachate loadings were up to approximately 10% of agroecosystem N inputs. The coincidence of (i) soluble N production and availability and (ii) preferential leaching pathways formed by deep-burrowing earthworms thereby increased N losses from the CT agroecosystem at the 45-cm depth. Processing of N compounds and transport in soil water from RT cropping were more affected by management phase and largely independent of earthworm activity.     

Methane emissions of rice increased by elevated carbon dioxide and temperature

Methane (CH4) effluxes by paddy-culture rice (Oryza sativa L.) contribute about 16% of the total anthropogenic emissions. Since radiative forcing of CH4 at current atmospheric concentrations is 21 times greater on a per mole basis than that of carbon dioxide (CO2), it is imperative that the impact of global change on rice CH4 emissions be evaluated. Rice (cv. IR72) was planted in sunlit, closed-circulation, controlled-environment chambers in which CH4 efflux densities were measured daily. The CO2 concentration was maintained at either 330 or 660 micromol mol(-1). Air temperatures were controlled to daily maxima and minima of 32/23, 35/26, and 38/29 degrees C at each CO2 treatment. Emissions of CH4 each day were determined during a 4-h period after venting and resealing the chambers at 0800 h. Diurnal CH4 effluxes on 77, 98, and 119 d after planting (DAP) were obtained similarly at 4-h intervals. Emissions over four-plant hills and over flooded bare soil were measured at 53, 63, and 100 DAP. Emissions were negligible before 40 DAP. Thereafter, emissions were observed first in high-CO2, high-temperature treatments and reached a sustained maximum efflux density of about 7 mg m(-2) h(-1) (0.17 g m(-2) d(-1)) near the end of the growing season. Total seasonal CH4 emission was fourfold greater for high-CO2, high-temperature treatments than for the low-CO2, low-temperature treatment, probably due to more root sloughing or exudates, since about sixfold more acetate was found in the soil at 71 DAP. Both rising CO2 and increasing temperatures could lead to a positive feedback on global warming by increasing the emissions of CH4 from rice.     

Evidence for differential selection and potential adaptive evolution in the worker caste of an inquiline social parasite

Social parasites exploit the socially managed resources of social insect colonies in order to maximise their own fitness. The inquilines are among the most specialised social parasites, because they are dependent on being fully integrated into their host's colony throughout their lives. They are usually relatives of their host and so share ancestral characteristics (Emery's rule). Closely related inquiline-host combinations offer a rare opportunity to study trade-offs in natural selection. This is because ancestral adaptations to a free-living state (e.g. the production of a worker caste) become redundant and may be replaced by novel, parasitic traits as the inquiline becomes more specialised. The dynamics of such processes are, however, unknown as virtually all extant inquiline social parasites have completely lost their worker caste. An exception is Acromyrmex insinuator, an incipient permanent social parasite of the leaf-cutting ant Acromyrmex echinatior. In the present study, we document the size distribution of parasite and host workers and infer how selection has acted on A. insinuator to reduce, but not eliminate, its investment in a worker caste. We show that the antibiotic producing metapleural glands of these parasite workers are significantly smaller than in their host counterparts and we deduce that the metapleural gland size in the host represents the ancestral state. We further show experimentally that social parasite workers are more vulnerable to the general insect pathogenic fungus Metarhizium than are host workers. Our findings suggest that costly disease resistance mechanisms are likely to have been lost early in inquiline evolution, possibly because active selection for maintaining these traits became less when parasite workers had evolved the ability to exploit the collective immune system of their host societies.     

Inventory of ocean monitoring in the Southern California Bight

Monitoring of the ocean environment in southern California, USA, has been conducted by a diverse array of public and private organizations with different motivations, working on a variety of spatial and temporal scales. To create a basis from which to integrate information from these diverse programs, we conducted an inventory of ocean monitoring activities in the Southern California Bight to address the following questions: (1) How much money is being expended annually on marine monitoring programs? (2) Which organizations are conducting the most monitoring? (3) How are resources allocated among the different types of monitoring programs? This inventory focused on programs existing, or those expected to be in existence, for at least 10 years and that were active at any time between 1994 and 1997. For each program identified for inclusion in this study, information was collected on the number of sites, sampling intensity, parameters measured, and methods used. Levels of effort were translated into cost estimates based upon a market survey of local consulting firms. One hundred fourteen marine monitoring programs, conducted by 65 organizations and costing US $31 million annually, were identified. Most of the effort (81 programs, 65% of samples, 70% of costs) was expended by ocean dischargers as part of their compliance with National Pollutant Discharge Elimination System (NPDES) permit requirements. Federal programs (11 programs, 25% of samples, 10% of total expenditures) expended more than state or local government programs. More than one quarter of monitoring expenditures were conducted to measure concentrations and mass of effluent inputs to the ocean. The largest effort expended on receiving water monitoring was for measuring bacteria, followed by sediments, fish/shellfish, water quality, and intertidal habitats. The large level of expenditures by individual agencies has presented opportunities for integrating small, site-specific ocean monitoring programs into regional- and national-scale monitoring and assessment programs.     

CHANNEL CHANGES DOWNSTREAM FROM A DAM1

ABSTRACT: A flood-control dam was completed during 1979 on Bear Creek, a small tributary stream to the South Platte River in the Denver, Colorado, area. Before and after dam closure, repetitive surveys between 1977 and 1992 at five cross sections downstream of the dam documented changes in channel morphology. During this 15-year period, channel width increased slightly, but channel depth increased by more than 40 percent. Within the study reach, stream gradient decreased and median bed material sizes coarsened from sand in the pools and fine gravel on the rime to a median coarse gravel throughout the reach. The most striking visual change was from a sparse growth of streamside grasses to a dense growth of riparian woody vegetation.