Stress and mental strain in hospital work: Exploring the relationship beyond personality

This study focuses on the relationship between job stressors and mental strain in hospital work, adjusting for differences in personality traits. Questionnaires were obtained from 760 full-time employees. Fourteen scales of job stressors were clustered into four factors: professional latitude, clinical demands, workload problems and role difficulties. A mental strain index was derived from the addition of three sub-scales: depression, anxiety and irritation. Two personality traits were measured: Locus of Control (Rotter) and Striver-Achiever (Sales). The four job demand factors were dichotomized into high-low grouping variables. A 2⁴ factorial analysis of covariance was conducted, using the two personality traits as covariates, in order to test and adjust for trait-related strain. Results show that Locus of Control adjusted differences in mental strain appear significantly related to work stressors. A direct relationship was found with role difficulties, professional latitude and workload problems. Moreover, high levels of role difficulties and low levels of professional latitude interact significantly with adjusted levels of mental strain. The Striver-Achiever trait, however, appears most strongly correlated with workload problems but not with mental strain. We believe that this exploratory analysis suggests that the stressor-strain relationship might be best conceived as a combination of direct and complex pathways, relating facets of both job demands and of personality with outcomes, under a cognitive and conditional paradigm.     

Monitoring the progress of attempts to reduce nutrient load and inputs of certain compounds in the north sea by 50%

During the winter period an inverse linear relation is found between the concentration of dissolved nutrients (phosphorus and nitrogen) and salinity in the Dutch coastal zone. This indicates a conservative behavior of these compounds from the river, through the estuary to the sea. During summer this relation is much more scattered because of biological processes. The physical and statistical properties of the relationship between salinity and the concentration of dissolved inorganic phosphorus and nitrogen are used to calculate when, where, and how many samples have to be taken in order to monitor a reduction of a compound accurately. It appeared that at any given salinity in the estuary and in the sea, the winter period is the most suitable season to detect a reduction of a given dissolved compound. The higher the salinity in the estuary, the more samples are required to prove the reduction significantly. A reduction of only 10% cannot even be demonstrated by field measurements during summer at salinities above 25. It is concluded that one cruise from the river to the sea, covering the salinities from 0 to 35 during the winter period, aimed at establishing the relationship between the concentration and salinity by taking samples at a salinity interval of for instance 1%, is sufficient to monitor a wide-ranging reduction of 10%–50% in both the fresh water and marine water. This program must be combined with a sampling at a salinity of 0, directed to determine the riverine temporal variability.     

Early second-trimester diagnosis of sirenomelia

Two cases of sirenomelia are described, detected in the 14th and 16th weeks of gestation by transvaginal ultrasonography. A hypothesis for the aetiology of sirenomelia and its associated anomalies is discussed.     

Prenatal diagnosis of Hunter syndrome using fetal plasma

The X-linked Hunter syndrome or mucopolysaccharidosis II was diagnosed in a male fetus by demonstrating a severe deficiency of iduronate 2-sulphate sulphatase activity in fetal plasma obtained by umbilical fetal blood sampling at 23 weeks of pregnancy. The diagnosis was confirmed after termination of pregnancy.     

Polymerase chain reaction analysis of the cystic fibrosis ΔF508 mutation in human blastomeres following oocyte injection of a single sperm from a carrier

The efficiency of the polymerase chain reaction (PCR) in detecting the cystic fibrosis (CF) ΔF508 mutation (which is the most common mutation of CF) was assessed in single human blastomeres. Twenty-one human immature oocytes (germinal-vesicle-stage oocytes) that had been donated for research were matured in vitro and a single spermatozoon from a carrier of the CF ΔF508 mutation was injected into the ooplasm. Fourteen embryos were obtained after intracytoplasmic sperm injection (ICSI). PCR analysis was carried out on 70 single blastomeres isolated from these 14 embryos. The results showed that the efficiency of DNA amplification by PCR in single nucleate blastomeres was 94 per cent (59/63). There were no false-positive results since none of the blank samples or the blastomeres without a nucleus showed an amplified signal. We found that nine embryos were homozygous for the unaffected genotype and that four embryos were heterozygous since they contained both the unaffected and the ΔF508 genotype. In a four-cell embryo, we observed the homozygous unaffected genotype in one blastomere and a heterozygous ΔF508/unaffected genotype in the other three blastomeres.     

Optimization, equilibrium, adsorption behavior and role of surface functional groups on graphene oxide-based nanocomposite towards diclofenac drug

Aquatic contamination of diclofenac (DCF), an emergent non-steroidal anti-inflammatory drug (NSAIDs), can result in adverse effects to many ecosystems through biomagnification. Hence, introducing effective remediation techniques to sequester the pharmaceutical wastes is highly fundamental to prevent their accumulation in the environment. Generally, adsorption has been presented as a green and efficient approach. Herein, we report the characterization and application of the novel magnetic nanocomposite ([email protected]₂O₄) derived from cobalt-based ferrite (CoFe₂O₄) and graphene oxide (GO) for DCF adsorption. For the optimization procedure, the response surface methodology (RSM) was adopted to investigate the impacts of DCF concentration (1.6–18.4 mg/L), DCF dosage (0.08–0.92 g/L), and solution pH (2.6–9.4) to find the optimum conditions for DCF removal, at 10.5 mg/L, 0.74 g/L, and pH 4, respectively. For the adsorption experiments, the kinetic, isotherm, thermodynamic, and intraparticle diffusion models were systematically studied. Moreover, we have elucidated the role of functional groups on the surface of [email protected]₂O₄ in enhancing the adsorption of DCF drug. With good removal efficiency (up to 86.1%), high maximum adsorption capacity (32.4 mg/g), [email protected]₂O₄ can be a potential candidate to eliminate DCF drug from water.     

Prenatal ultrasonographic diagnosis of radial-ray reduction malformations

Radial-ray reduction malformations (RRRMs) may occur isolated or in association with other anomalies. The data of seven fetuses born with RRRMs were collected. Six fetuses had associated lethal abnormalities of the central nervous system, urogenital system, and/or heart, detected by ultrasound. In five cases, it was possible to establish the precise diagnosis, enabling an informed prognosis and subsequent genetic counselling. The diagnoses were: Edwards syndrome (n=3), VACTERL association (n=1), and Poland-Moebius-like complex (n= 1). In two cases, a complete diagnosis was not possible because of inadequate evaluation of these fetuses before and/or after birth. A proposal is given for the diagnostic approach for infants with RRRMs detected in the antenatal period by means of ultrasonography.     

Increased maternal serum alpha-fetoprotein and human chorionic gonadotropin in compromised pregnancies other than for neural tube defects or Down syndrome

Intrauterine fetal death occurred in four women who were ‘screen-positive’ in a screening programme for neural tube defects (NTDs) and Down syndrome (DS). These women had very high levels of maternal serum alpha-fetoprotein (MSAFP) and maternal serum human chorionic gonadotropin (MShCG). Therefore, we evaluated all ‘screen-positive’ women in whom both of these markers were ⩾ 2.0 multiples of the median. The cases fulfilling these criteria totalled 11, and only one of them had no complications. High concentrations of both MSAFP and MShCG in a number of these cases might have been caused by an increased placental volume, which, in turn, might have been induced by decreased perfusion of the placenta. We conclude that screening programmes wrongly determine a high risk of fetal NTD or DS if the concentrations of both these parameters are very high. Invasive diagnostic procedures should be avoided in these cases, particularly in view of the increased risk of an adverse pregnancy outcome.     

Anti-grazing activity and seasonal variation of dimethylsulfoniopropionate-associated compounds in the invasive alga <Emphasis Type="Italic">Codium fragile</Emphasis> ssp. <Emphasis Type="Italic">tomentosoides</Emphasis>

In this study, we present evidence that the invasive alga Codium fragile ssp. tomentosoides is chemically defended against grazing by a wound-activated chemical defense involving dimethylsulfoniopropionate (DMSP) and the products of its cleavage, dimethylsulfide (DMS), and acrylic acid (AA). DMSP in C. fragile ssp. tomentosoides was present throughout the year, but concentrations varied seasonally and were highest in the winter. Intra-thallus variation was neither large, nor consistent over time. High DMSP concentrations were uncommon among northwestern Atlantic macrophytes. Of 26 other species tested, only two, Ulva lactuca and Polysiphonia harveyi contained concentrations comparable to, or higher than, those of C. fragile ssp. tomentosoides. DMS and AA, both individually and together, deterred grazing by the green sea urchin Strongylocentrotus droebachiensis at “natural” concentrations. These results suggest that DMS and AA contribute to the avoidance of C. fragile ssp. tomentosoides by S. droebachiensis. As a result, the production of DMSP and its subsequent cleavage, upon injury, may reduce herbivory on C. fragile ssp. tomentosoides and contribute to its success.     

Spatial and interannual variation in the faunal distribution at Broken Spur vent field (29°N, Mid-Atlantic Ridge)

Initial ecological observations at Broken Spur in 1993 suggested a low biomass relative to other deep-water vent communities known along the Mid-Atlantic Ridge. The persistence of a low shrimp biomass over 15 mo at Broken Spur vents appears to refute the hypothesis that the community is expanding through reproduction and immigration to occupy habitat vacated during a recent period of hydrothermal quiescence or catastrophe. Although the absence of “swarms” of shrimp similar to those found at the hydrothermal mounds of TAG (26°N) and Snake Pit (23°N) is a visually striking feature of the majority of venting structures known at Broken Spur, the biomass of fauna other than shrimp may not be significantly less than that of other Mid-Atlantic sites. The discovery of “swarms” of shrimp at Bogdanov Site, visited for the first time in 1994, suggests that availability of substratum exposed to the flow of hydrothermal fluids, which is a function of the topography of venting structures, may be a prerequisite for the development of these dense aggregations. Two testable predictions arise from this hypothesis. Firstly, dense aggregations of Rimicaris exoculata should occur at any other structures with a morphology similar to Bogdanov Site that may be discovered in the Broken Spur vent field, and should not occur at other isolated chimney structures that may be found. Secondly, “swarms” of shrimp should appear at any Broken Spur chimneys that develop into structures with a morphology more similar to that of Bogdanov Site in future. Received: 2 July 1997 / Accepted: 9 July 1997