No evidence for genetic differentiation between Antarctic limpet Nacella concinna morphotypes

The extent to which genetic divergence can occur in the absence of physical barriers to gene flow is currently one of the most controversial topics in evolutionary biology, with implications for our understanding of speciation, phenotypic plasticity and adaptive potential. This is illustrated by a recent study reporting a surprising pattern of genetic differentiation between intertidal and subtidal morphotypes of the broadcast-spawning Antarctic limpet Nacella concinna. To explore this further, we collected almost 400 Antarctic limpets from four depths (intertidal, 6, 15 and 25 m) at Adelaide island, Antarctica, and conducted a combined morphometric and genetic analysis using 168 polymorphic amplified fragment length polymorphism (AFLP) loci. Morphological analysis revealed not only pronounced differences between the two morphotypes, but also a continuous cline in shell shape from the intertidal zone down to 25 m depth, suggesting that the distinction between the morphotypes may be artificial. Moreover, genetic analysis using both F _st and a Bayesian analogue found no evidence for differentiation either between the two morphotypes or by depth, and a Bayesian cluster analysis did not detect any cryptic genetic structure. Our findings lend support to the notion that limpets can be phenotypically highly plastic, although further studies are required to determine unequivocally whether there is any genetic basis to the observed variation in shell morphology.     

IDENTIFICATION OF DISSOLVED-CONSTITUENT SOURCES IN MINE-SITE GROUND WATER USING BATCH MIXING1

ABSTRACT: Batch-mixing experiments were used to help identify lithologic and mineralogic sources of increased concentrations of dissolved solids in water affected by surface coal mining in northwestern Colorado. Ten overburden core samples were analyzed for mineral composition and mixed with distilled water for 90 days until mineral-water equilibrium was reached. Between one day and 90 days after initial contact, specific conductance in the sample mixtures had a median increase of 306 percent. Dissolved-solids concentrations ranged from 200 to 8,700 mg/L in water samples extracted from the mixtures after 90 days. Mass. balance simulations were conducted using the geochemical models BALANCE and WATEQF to quantify mineral-water interactions occurring in five selected sample mixtures and in water collected from a spring at a reclaimed mine site. The spring water is affected by mineral-water interactions occurring in all of the lithologic units comprising the overburden. Results of the simulations indicate that oxidation of pyrite, dissolution of dolomite, gypsum, and epsomite, and cation-exchange reactions are the primary mineral-water interactions occurring in the overburden. Three lithologic units in the overburden (a coal, a sandstone, and a shale) probably contribute most of the dissolved solids to the spring water. Water sample extracts from mixtures using core from these three units accounted for 85 percent of the total dissolved solids in the 10 sample extracts. Other lithologic units in the overburden probably contribute smaller quantities of dissolved solids to the spring water.     

CONFLICTING PERSPECTWES ABOUT DETECTION LIMITS AND ABOUT THE CENSORING OF ENVIRONMENTAL DATA1

ABSTRACT: Current conventions for reporting analytical results from environmental samples brings the objectives of laboratory scientists into conflict with those of environmental scientists. The objective of chemical analyses is to provide estimates of the true composition of samples. Reported results must reflect the analytical uncertainty. Current conventions require left-censoring of those results below the Limit of Detection. The objective of statistical interpretation of environmental data is to provide estimates of the characteristics of ecosystems. Such statistical analyses are often confounded by left-censoring of analytical results. We review the different points of view and propose a compromise which recognizes these conflicting perspectives.     

Resolving conflicting priorities in Ontario agriculture

Changes in global patterns of grain production have affected the profitability of commercial, cash-crop agriculture in North America. The current financial crisis has highlighted a perceived conflict between the priorities of (1) strengthening net farm profit, (2) maintaining the productive potential of the land base, (3) enhancing the health and cohesiveness of the agricultural community, and (4) addressing societal demands for safe foodstuffs. Reducing input costs by reducing the need for privately owned machinery can minimize the scale-dependence of agricultural practices, as illustrated with examples involving silage and intensively managed pasture in Ontario. This approach could improve farming opportunities for nontraditional, part-time farmers, and at the same time, create a niche for professional custom operators and managers. Enhancing the viability of nontraditional farm operations, a historically neglected component of the farming community, as well as commercial farms is viewed as one approach to sustaining and improving both the agricultural land base and the agricultural community. Applying resource-extensive rather than resource-intensive approaches to forage management reveals that these apparently divergent priorities are, in fact, interlocking pieces of the same puzzle.     

Making Sense of the Policy Process for Carnivore Conservation

Grizzly bears ( Ursus arctos horribilis ), mountain lions ( Puma concolor ), wolverines ( Gulo gulo ), wolves ( Canis lupus ), and lynx ( Felis lynx ) are all top-level carnivores in the Rocky Mountains of the northern U.S. and southern Canada. Because of their body size and high trophic position, these species require abundant prey species and large habitat areas—requirements that make their conservation a controversial public policy problem. Because the challenge of conserving these species extends beyond biological issues, it is necessary to involve other relevant disciplines and perspectives in understanding and solving the problem. Our examination of the context, content, and process of large carnivore conservation policies suggests more effective and active roles for scientists in designing solutions to the problem of landscape-level carnivore conservation. Scientists must develop an understanding of the range of participants in the policy process and the ways in which these participants receive and utilize information. This knowledge of the policy process could help scientists to better understand their roles in framing and clarifying policy questions, projecting the consequences of various alternatives, and presenting policy information in appropriate fora.