Isolation and identification of Aeromonas caviae strain KS-1 as TBTC- and lead-resistant estuarine bacteria

Tributyltin chloride (TBTC)- and lead-resistant estuarine bacterium from Mandovi estuary, Goa, India was isolated and identified as Aeromonas caviae strain KS-1 based on biochemical characteristics and FAME analysis. It tolerates TBTC and lead up to 1.0 and 1.4 mM, respectively, in the minimal salt medium (MSM) supplemented with 0.4 % glucose. Scanning electron microscopy clearly revealed a unique morphological pattern in the form of long inter-connected chains of bacterial cells on exposure to 1 mM TBTC, whereas cells remained unaltered in presence of 1.4 mM Pb(NO₃)₂ but significant biosorption of lead (8 %) on the cell surface of this isolate was clearly revealed by scanning electron microscopy coupled with energy dispersive X-ray spectroscopy. SDS-PAGE analysis of whole-cell proteins of this lead-resistant isolate interestingly demonstrated three lead-induced proteins with molecular mass of 15.7, 16.9 and 32.4 kDa, respectively, when bacterial cells were grown under the stress of 1.4 mM Pb (NO₃)₂. This clearly demonstrated their possible involvement exclusively in lead resistance. A. caviae strain KS-1 also showed tolerance to several other heavy metals, viz. zinc, cadmium, copper and mercury. Therefore, we can employ this TBTC and lead-resistant bacterial isolate for lead bioremediation and also for biomonitoring TBTC from lead and TBTC contaminated environment.     

Fishers’ perceptions of climate change,impacts on their livelihoods and adaptation strategies in environmental change hotspots: a case of Lake Wamala,Uganda

Fisheries resources support livelihoods of fishing communities but are threatened by over-exploitation, habitat degradation, pollution, invasive species and climate change. Unlike the other threats, climate change has received limited consideration and reducing its risks requires appropriate adaptation strategies. This study used quantitative and qualitative methods to generate knowledge on fishers’ perceptions of climate change, changes in climate variables and their impacts on livelihoods, adaptation strategies, constraints to adaptation and required interventions to promote adaptation strategies that would enable fishers to build resilience to sustain their livelihoods. We found that fishers were aware of changes in climate conditions manifested by unpredictable seasons, floods and droughts. Fishing remained the main livelihood activity. However, the dominance of fishes had changed from Nile tilapia (Oreochromis niloticus L.) to the African catfish (Clarias gariepinus Burchell). Floods and droughts were associated with damage to gears, boats, landing sites and changes in fish catches and sizes, income from fishing and fish consumption. The fishers adapted by increasing time on fishing grounds and changing target species and fishing gear among other things. Some innovative fishers diversified to high-value crops and livestock. This increased their income beyond what was solely earned from fishing which provided an incentive for some of them to quit fishing. Livelihood diversification was enhanced by use of communications technology, membership of social groups, increasing fishing days and fishing experience. Adaptation was, however, constrained by limited credit, awareness and access to land, which require interventions such as improving access to credit, irrigation facilities, appropriate planting materials and awareness raising. We identified adaptation strategies, which if promoted and their constraints addressed, could increase resilience of fishers to the influence of climate change and sustain their livelihoods.     

FF/C Scrubber Demonstration on a Secondary Metals Recovery Furnace

A flux force/condensation (FF/C) scrubbing system was built to control participate emissions from a secondary metals recovery furnace. Total mass penetration and fractional penetration measurements were made under several different operating modes. The performance of the demonstration scrubber was consistent with the results of previous studies on FF/C scrubbing. The system was generally capable of 90 to 95 % efficiency on particles with a mass median aerodynamic diameter of 0.75 µA. This efficiency was achieved with a 68 cm (27 in.) W.C. gas-phase pressure drop.