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981.
Chen G Wu X Zhou WL Li L 《Journal of environmental biology / Academy of Environmental Biology, India》2010,31(5):655-660
C-glucosyltransferase (EC 2.4.1.X) is one of the key enzymes for the biosynthesis of puerarin. This paper describes the methodology in purification and assay of the enzyme for the first time in Puerarin lobata (Wild.) Ohwi. C-glucosyltransferase from roots of P. lobata was extracted and partially purified by (NH4)2SO4 saturation. The effects of pH, temperature, and substrate concentration on the activity of the enzyme were investigated. The properties of the puerarin produced by C-glucosyltransferase were studied by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The peak activity of C-glucosyltransferase was detected in fraction of by 80% saturation of (NH4)2SO4 and the optimal conditions for enzymatic reaction were 35.5 micromol l(-1) of isoliquiritigenin and 560 micromol l(-1) of UDP-G at pH 8.1, 28 degrees C for 1 h. Mn2+ at 1 mmol l(-1) and Al3+ at 1 mmol l(-1) increased the enzyme activity, while Mg2+ inhibited its activity. The enzyme activity in Nicotiana tabacum and P. lobata were detected under the above assay conditions. Higher activity was found in roots than in leaves and stems of P. lobata, while no enzyme activity was detected in leaves of N. tabacum. It was the first time that activity of C-glucosyltransferase, which transforms isoliquiritigenin to puerarin, was detected in P. lobata. 相似文献
982.
983.
Qian YAO Hua ZHANG Jun WU Liming SHAO Pinjing HE 《Frontiers of Environmental Science & Engineering》2010,4(3):286-294
Batch biosorption experiments were conducted to remove Cr(III) from aqueous solutions using activated sludge from a sewage treatment plant. An investigation was conducted on the effects of the initial pH, contact time, temperature, and initial Cr(III) concentration in the biosorption process. The results revealed that the activated sludge exhibited the highest Cr(III) uptake capacity (120 mg·g−1) at 45°C, initial pH of 4, and initial Cr(III) concentration of 100 mg·L−1. The biosorption results obtained at various temperatures showed that the biosorption pattern accurately followed the Langmuir model. The calculated thermodynamic parameters, ΔGo (−0.8– −4.58 kJ·mol−1), ΔHo (15.6–44.4 kJ·mol−1), and ΔSo (0.06–0.15 kJ·mol−1·K−1) clearly indicated that the biosorption process was feasible, spontaneous, endothermic, and physical. The pseudo first-order and second-order kinetic models were adopted to describe the experimental data, which revealed that the Cr(III) biosorption process conformed to the second-order rate expression and the biosorption rate constants decreased with increasing Cr(III) concentration. The analysis of the values of biosorption activation energy (Ea = −7 kJ·mol−1) and the intra-particle diffusion model demonstrated that Cr(III) biosorption was film-diffusion-controlled. 相似文献
984.
Shuting ZHANG Bo WEI Xin YU Bing LIU Zhuoying WU Li GU 《Frontiers of Environmental Science & Engineering》2010,4(4):459-465
The biologic activated carbon (BAC) process is widely used in drinking water treatments. A comprehensive molecular analysis of the microbial community structure provides very helpful data to improve the reactor performance. However, the bottleneck of deoxyribonucleic acid (DNA) extraction from BAC attached biofilm has to be solved since the conventional procedure was unsuccessful due to firm biomass attachment and adsorption capacity of the BAC granules. In this study, five pretreatments were compared, and adding skim milk followed by ultrasonic vibration was proven to be the optimal choice. This protocol was further tested using the vertical BAC samples from the full-scale biofilter of Pinghu Water Plant. The results showed the DNA yielded a range of 40 μg·g-1 BAC (dry weight) to over 100 μg·g-1 BAC (dry weight), which were consistent with the biomass distribution. All results suggested that the final protocol could produce qualified genomic DNA as a template from the BAC filter for downstream molecular biology researches. 相似文献
985.
Jinbao WU Zongqiang GONG Liyan ZHENG Yanli YI Jinghua JIN Xiaojun LI Peijun LI 《Frontiers of Environmental Science & Engineering》2010,4(4):387-394
Solubilizing experiments were carried out to evaluate the ability of biodiesel to remove polycyclic aromatic hydrocarbons (PAHs) from highly contaminated manufactured gas plant (MGP) and PAHs spiked soils with hydroxypropyl-β-cyclodextrin (HPCD) and tween 80 as comparisons. Biodiesel displayed the highest solubilities of phenanthrene (420.7 mg·L-1), pyrene (541.0 mg·L-1), and benzo(a)pyrene (436.3 mg·L-1). These corresponded to several fold increases relative to 10% HPCD and tween 80. Biodiesel showed a good efficiency for PAH removal from the spiked and MGP soils for both low molecular weight and high molecular weight PAHs at high concentrations. Biodiesel was the best agent for PAH removal from the spiked soils as compared with HPCD and tween 80; as over 77.9% of individual PAH were removed by biodiesel. Tween 80 also showed comparable capability with biodiesel for PAH solubilization at a concentration of 10% for the spiked soils. Biodiesel solubilized a wider range of PAHs as compared to HPCD and tween 80 for the MPG soils. At PAH concentrations of 229.6 and 996.9 mg·kg-1, biodiesel showed obvious advantage over the 10% HPCD and tween 80, because it removed higher than 80% of total PAH. In this study, a significant difference between PAH removals from the spiked and field MGP soils was observed; PAH removals from the MGP soil by HPCD and tween 80 were much lower than those from the spiked soil. These results demonstrate that the potential for utilizing biodiesel for remediation of highly PAH-contaminated soil has been established. 相似文献
986.
基于地理探测器的区域植被覆盖度的定量影响——以京津风沙源区为例 总被引:1,自引:0,他引:1
植被覆盖度(FVC)是衡量生态环境优秀的重要指标.本文基于MODIS NDVI数据,运用像元二分法模型,估算了2000~2018年京津风沙源区FVC,分析了其时空变化特征.在此基础上,基于地理探测器模型,定量分析了自然因素和人类活动因素等12个因子对京津风沙源区FVC空间分布的影响.结果表明,近20a来京津风沙源区FVC整体上呈增加趋势,增加率为8.2%.空间上,研究区73.7%的区域FVC在增加,其中显著增加的区域主要集中在晋北山地丘陵亚区、燕山丘陵山地水源保护亚区、大兴安岭南部亚区和科尔沁沙地亚区的南部.2000~2018年,降水是影响京津风沙源区FVC空间分布的主要自然因子,解释力为63.3%;年末大牲畜头数是影响FVC空间分布的主要人为因子,解释力为34.8%.自然因素和人类活动之间的双因子交互作用以双协同作用和非线性协同作用为主,表明相对于单因子,自然因素和人类活动间的交互作用对FVC的解释力更强.其中,降水与其他11个因子的交互作用对研究区FVC起主导作用,解释力超过60%. 相似文献
987.
随着经济的不断发展,越来越多的国家和地区开始重视绿色高质量发展,对生态产品的研究也在不断加强。各种政策工具相继出现,致力于将自然给予的生态产品资本化,并赋能经济发展。其中,衢州市柯城区的“一村万树”项目就是一种具有创新性的生态产品价值实现模式。本文对衢州市柯城区“一村万树”项目及其绿色期权模式进行研究,结合部门统计数据和空间信息数据,对“一村万树”项目实施区2018年的生态效益进行核算,并选取柯城区2010—2018年相关社会经济统计数据进行相关性分析,探析项目的社会经济效益,最后在量化分析的基础上结合公共池塘资源治理的八条设计原则,定性分析了“一村万树”绿色期权的合理性。结果显示:“一村万树”实施区2018年的生态效益为124.63亿元,约为研究区国内生产总值估算值的1.68倍,说明该项目具有巨大的生态效益;同时,相关分析结果表明该项目实施对当地第三产业的发展有较大的促进作用。柯城区“一村万树”项目以及绿色期权模式给当地带来了巨大的生态和社会经济效益,为我国的乡村绿化建设和生态产品价值实现提供了一种新范式。 相似文献
988.
989.
灰色预测是环境污染预测应用相当广泛的方法。根据文献报道,基于Matlab和Excel实现灰色预测中存在着占用内存、单元格填埋计算公式等等的不足,因此提出了一种基于VBA在Excel下编写宏程序轻松实现灰色预测的方法,并将其运用到北海市地表水污染物预测。结果表明,本程序简短,操作方便,计算结果准确可靠,彻底把用户从繁琐的手工操作中解放出来。 相似文献
990.
吴玉红 《中国环境管理干部学院学报》2013,(3):80-83
基于工作过程的项目化教学是高职院校课程改革的方向,以《环境规划与评价GIS技术》课程为例,探讨了高职GIS应用类课程项目化教学的改革与实践,主要内容包括课程设计思路、教学目标、课程内容选取、项目化教学实施与课程考核等。实践证明项目化教学改革取得了良好的教学效果。 相似文献