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671.
刘林敏邱子文叶清华谢倩王威陈清西 《应用与环境生物学报》2022,(6):1503-1509
糖外排转运蛋白(sugars will eventually be exported transporters,SWEET)介导植物光合同化产物蔗糖的跨膜运输.以橄榄(Canarium album(Lour.)Raeusch.)果实为材料,通过气相色谱串联质谱(GC-MS)检测橄榄果实中糖组分及含量变化,利用RT-PCR技术克隆得到CaSWEET7和CaSWEET15基因开放阅读框(ORF)序列.结果表明:CaSWEET7和CaSWEET15基因ORF全长分别为774 bp和951 bp,分别编码长度为257个和316个氨基酸残基,具有2个MtN3_slv结构域;进化树分析表明,CaSWEET7属于CladeⅡ,CaSWEET15属于CladeⅢ.实时荧光定量PCR(qRT-PCR)技术检测其在不同发育时期的果实中相对表达量变化,结果表明,随着果实发育,CaSWEET7和CaSWEET15表达量逐渐递增,且与果实发育蔗糖含量变化呈显著正相关(相关性系数分别为0.931和0.904,P<0.05);利用酵母功能互补证明CaSWEET7和CaSWEET15基因编码的蛋白具有转运蔗糖能力.本研究表明CaSWEET7和CaSWEET15基因可能在橄榄果实蔗糖积累中发挥作用.(图9表2参44) 相似文献
672.
Level and distribution of DDT in surface soils from Tianjin, China 总被引:28,自引:0,他引:28
Gong ZM Tao S Xu FL Dawson R Liu WX Cui YH Cao J Wang XJ Shen WR Zhang WJ Qing BP Sun R 《Chemosphere》2004,54(8):1247-1253
One hundred and eighty eight surface soil samples were collected from the Tianjin area to study the contamination of DDT and its metabolites. Measurements were taken for p,p'-DDE, p,p'-DDD, p,p'-DDT, o,p'-DDE, o,p'-DDD and o,p'-DDT for all samples. The results indicated that p,p'-DDT and p,p'-DDE were the predominant contaminant compounds in the surface soil samples, with mean concentrations of 27.5 and 18.8 ng g(-1) respectively. No significant differences in DDT concentrations were found between the soils from wastewater treated irrigated areas and other areas, suggesting that wastewater irrigation is not an important source of DDT in the area. However, the spatial distribution of soil DDTs levels in the area did correlate well with early direct application rates of pesticides. In addition, both pH level and organic carbon content are also known factors affecting the level of DDT and its metabolites. Although it was assumed that the use of these chemicals was banned in the early 1980s, the current concentration levels appear to be too high to be mere residuals after 20 years degradation. 相似文献
673.
The use of organo-chlorine (DDT and HCH) has been banned in China for 20 years. A field survey was carried out during 1999-2000 in the Taihu Lake Region. Organo-chlorine pesticide (OCP) residues in soil, water, fish and sediment samples were investigated. DDT was detected in 5 out of 10 samples with concentration ranging from 0.3 to 5.3 microg/kg in the surface (0-15 cm) layer, 6 out of 10 with 0.5 to 4.0 microg/kg in the subsoil layer (16-30 cm), and 4 of 10 with 0 to 2.7 microg/ kg in the deep soil layer (31-50 cm). Results for HCH residues in soil samples were similar to those of DDT. These results indicate that OCP residues in 0-50 cm profile had been leached out or degraded to safe level. In river water DDT was detected in 10 out of 13 samples ranging from 0.2 to 9.3 microg/l, with an average of 1.0 microg/l. While HCH was detected in 12 out of 13 samples ranging from 0.02 to 36.1 microg/l, with an average 5.6 microg/l. DDT residues in sediment ranged from 0.1 to 8.8 microg/kg, while HCH ranged from 0.3 to 66.5 microg/kg. DDT residues in fish body ranged from 3.7 to 23.5 microg/kg and HCH ranged from 3.7 to 132 microg/kg. These results demonstrate an accumulation through food chain (from soil-water-sediment-microbes-crop-fish-... etc.), also that HCH residues are generally more persistent than DDT residues. However, all these data are well below than the state warning standard limit. 相似文献
674.
Accumulation and distribution of polycyclic aromatic hydrocarbons in rice (Oryza sativa) 总被引:6,自引:0,他引:6
Tao S Jiao XC Chen SH Liu WX Coveney RM Zhu LZ Luo YM 《Environmental pollution (Barking, Essex : 1987)》2006,140(3):406-415
Various tissues of rice plants were sampled from a PAH contaminated site in Tianjin, China at different growth stages of the ripening period and analyzed for PAHs. PAHs were much higher in roots than in the exposed tissues. Grains and internodes accumulated much smaller amounts of PAHs than leaves, hulls or ear axes. No specific gradient trends along roots, stem, ear axes, and grains were observed, suggesting that systematic translocation among them is unlikely. Over the ripening period, PAH concentrations were increased in rice roots and decreased in most above-ground tissues. Significant correlations between PAH and lipid contents can only be observed during full mature stage. The spectra of individual PAH compounds in rice organs including roots were similar to those in air, rather than those in soil. There was also a significant correlation between bioconcentration factor (BCF, plant over air) and octanol/air partitioning coefficient (Koa). 相似文献
675.
676.
能量桩与地基土的热交换取决于建筑物的年能源需求,故能量桩每年受到冷-热循环作用。采用多场耦合有限元数值模拟方法,研究在力学荷载和随时间按正弦函数变化的温度荷载共同作用下悬浮能量桩的热-力学特性。结果表明,随着能量桩冷-热周期性的循环,温度荷载引起的桩身附加轴向应力、桩头附加竖向位移和桩侧附加剪应力也随时间周期性变化,且相位与温度荷载曲线的相位相同。桩升温最大时桩身轴向压应力达到最大值,桩降温最大时桩头沉降达到最大值。地基土的温度改变量随时间周期性地变化,其幅值在桩的中部深度附近最大,在桩二端深度附近较小。地基土温度的变化滞后于温度荷载。离桩越远,地基土的温度达到其最大值的时间越滞后。 相似文献
677.
Ming W. Song Ping Huang Feng Li Hui Zhang Kai Z. Xie Xi H. Wang Guo X. He 《Environmental monitoring and assessment》2011,172(1-4):589-603
Water quality information of Beijiang River, a tributary of Pearl River in Guangdong, China, was analyzed to provide an overview of the hydrochemical functioning of a major agricultural/rural area and an industrial/urban area. Eighteen water quality parameters were surveyed at 13 sites from 2005 to 2006 on a monthly basis. A bivariate correlation analysis was carried out to evaluate the regional correlations of the water quality parameters, while the principal component analysis (PCA) technique was used to extract the most influential variables for regional variations of river water quality. Six principal components were extracted in PCA which explained more than 78% and 84% of the total variance for agricultural/rural and industrial/urban areas, respectively. Physicochemical factor, organic pollution, sewage pollution, geogenic factor, agricultural nonpoint source pollution, and accumulated pesticide usage were identified as potential pollution sources for agricultural/rural area, whereas industrial wastewaters pollution, mineral pollution, geogenic factor, urban sewage pollution, chemical industrial pollution, and water traffic pollution were the latent pollution sources for industrial/urban area. A multivariate linear regression of absolute principal component scores (MLR-APCS) technique was used to estimate contributions of all identified pollution sources to each water quality parameter. High coefficients of determination of the regression equations suggested that the MLR-APCS model was applicable for estimation of sources of most water quality parameters in the Beijiang River Basin. 相似文献
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680.
S. A. Murzina Z. A. Nefedova S. N. Pekkoeva N. N. Nemova 《Russian Journal of Ecology》2014,45(2):143-149
Using histological techniques, changes in histomorphological characteristics of the liver were revealed in two fish species from lakes with different levels of anthropogenic impact. The most significant changes in the liver of roach from Kostomukshskoe Lake (used as a tailing dump of the Kostomuksha Mining and Ore-processing Plant) included initial stages of parenchymal protein dystrophy and, to a lesser extent, fatty dystrophy; in pike, fatty liver dystrophy prevailed. Compared to roach, pike proved to be more sensitive to ecological conditions in Kostomukshskoe lake, including increased water mineralization. In both fish species from Koivas Lake (20 km from the pollution source), only the initial stages of local dystrophic changes could be detected. The fish from clean Kamennoe Lake (located in a protected area) had no pathological changes in the liver. 相似文献