Freshwater crayfish Astacus leptodactylus (Eschscholtz, 1823) accumulates and depurates copper

Cu accumulation and depuration in various tissues of the crayfish Astacus leptodactylus was investigated. Adult specimens were exposed to 0.5, 2.5 and 5.0 mg Cu/L under static conditions for three weeks. At the end of the 3rd week the specimens were divided into three groups and left in dechlorinated water for either 1, 2 or 3 weeks for depuration. After 7, 14 and 21 days, four crayfish from each group were instantaneously sacrificed. All crayfish were dissected into their hepatopancreas, gill, abdominal muscle and exoskeleton tissues for evaluation of Cu accumulation in each. The following accumulation pattern was obtained in decreasing order; hepatopancreas > gills > exoskeleton > abdominal muscles with values of 94.13, 84.86, 66.13 and 11.43 mg/L, respectively. The observed Cu depuration throughout the study was found to be time-dependent. Based on the present work we conclude that crayfish has a great potential for rapid accumulation and depuration of Cu in fresh water.     

新疆宏观生态环境质量综合评价研究——以布尔津县为例

新疆地处中国西北典型干旱内陆区,生态环境比较恶劣,建立适合新疆的省区一级生态环境质量综合评价指标体系,将对科学、客观了解新疆乃至整个西北干旱区生态环境状况都具有十分重要的理论和实践意义。采用综合指数法,从降水量、植被指数、生态功能价值、土壤侵蚀、降尘量、悬浮物等6个方面选取指标,分别以景观单元和县市为评价单元,建立了简易合理、具可操作性的不同尺度的新疆生态环境质量评价指标体系,并确定了各指标的权值和相对的分级标准,为新疆生态环境现状的准确评价提供了可靠依据,对新疆社会经济的可持续发展和生态环境管理将起到积极作用。     

水环境中抗生素的分布、累积及生态毒理效应

抗生素持续大量使用带来的环境污染和潜在的生态风险已经引起广泛关注。总结了国内外相关研究成果,对水环境中抗生素的污染源及残留水平进行了分析,对抗生素在水生生物体内的累积规律给予了评述,同时对抗生素的生态毒理效应进行了探讨。鉴于我国水环境中抗生素的污染现状,建议未来加强环境相关浓度下抗生素在生物体内的分布与转化、食物链迁移与放大,以及多种抗生素联合效应等方面的研究。     

Distribution and Fractionation of Phosphorus,Cadmium, Nickel,and Lead in Calcareous Soils Amended with Composts

Abstract

Composts improve organic carbon content and nutrients of calcareous soils but the accumulation and distribution of phosphorus and heavy metals among various fractions in soil may vary under the south Florida conditions. The accumulation of P, Cd, Ni, and Pb with depth and the distribution of water soluble, exchangeable, carbonate, Fe–Mn oxides, organic and residual forms of each element were investigated in soils amended with municipal solid waste (MSW) compost, co-compost and biosolids compost and inorganic fertilizer (as control). Total concentrations of P, Cd, Ni, and Pb were higher in the 0–22 cm soil layers and decreased considerably in the rock layers. These elements were in the decreasing order of P ? Pb > Ni > Cd. Amounts of water soluble and exchangeable forms of P, Cd, Ni and Pb were negligible at 0–22 cm soil depths except for Cd in the 10–22 cm depth. Amending calcareous soil with either organic or inorganic amendments rendered phosphorus, nickle and lead in the residual form followed by Fe–Mn oxides form in the 0–10 and 10–22 cm soil layers. Cadmium was predominantly in the Fe–Mn oxides fraction followed by the residual and carbonate forms in both soil layers. A significant positive correlation was found between various organic carbon fractions and organic forms of P, Cd and Pb in the surface soil layer. Soil amended with MSW compost had higher concentration of Cd in the organic fraction whereas, co-compost and MSW compost amended soil had higher concentrations of organic Ni fraction in the 0–10 cm soil layer.     

Accumulation of free and covalently bound microcystins in tissues of Lymnaea stagnalis (Gastropoda) following toxic cyanobacteria or dissolved microcystin-LR exposure

Accumulation of free microcystins (MCs) in freshwater gastropods has been demonstrated but accumulation of MCs covalently bound to tissues has never been considered so far. Here, we follow the accumulation of total (free and bound) MCs in Lymnaea stagnalis exposed to i) dissolved MC-LR (33 and 100 μg L⁻¹) and ii) Planktothrix agardhii suspensions producing 5 and 33 μg MC-LR equivalents L⁻¹ over a 5-week period, and after a 3-week depuration period. Snails exposed to dissolved MC-LR accumulated up to 0.26 μg total MCs g⁻¹ dry weight (DW), with no detection of bound MCs. Snails exposed to MCs producing P. agardhii accumulated up to 69.9 μg total MCs g⁻¹ DW, of which from 17.7 to 66.7% were bound. After depuration, up to 15.3 μg g⁻¹ DW of bound MCs were detected in snails previously exposed to toxic cyanobacteria, representing a potential source of MCs transfer through the food web.     

Arsenate tolerance in Silene paradoxa does not rely on phytochelatin-dependent sequestration

Arsenate tolerance, As accumulation and As-induced phytochelatin accumulation were compared in populations of Silene paradoxa, one from a mine site enriched in As, Cu and Zn, the other from an uncontaminated site. The mine population was significantly more arsenate-tolerant. Arsenate uptake and root-to-shoot transport were slightly but significantly higher in the non-mine plants. The difference in uptake was quantitatively insufficient to explain the difference in tolerance between the populations. As accumulation in the roots was similar in both populations, but the mine plants accumulated much less phytochelatins than the non-mine plants. The mean phytochelatin chain length, however, was higher in the mine population, possibly due to a constitutively lower cellular glutathione level. It is argued that the mine plants must possess an arsenic detoxification mechanism other than arsenate reduction and subsequent phytochelatin-based sequestration. This alternative mechanism might explain at least some part of the superior tolerance in the mine plants.     

Arsenic accumulation by the aquatic fern Azolla: comparison of arsenate uptake, speciation and efflux by A. caroliniana and A. filiculoides

This study investigates As accumulation and tolerance of the aquatic fern Azolla. Fifty strains of Azolla showed a large variation in As accumulation. The highest- and lowest-accumulating ferns among the 50 strains were chosen for further investigations. Azolla caroliniana accumulated two times more As than Azolla filiculoides owing to a higher influx velocity for arsenate. A. filiculoides was more resistant to external arsenate due to a lower uptake. Both strains showed a similar degree of tolerance to internal As. Arsenate and arsenite were the dominant As species in both Azolla strains, with methlyated As species accounting for <5% of the total As. A. filiculoides had a higher proportion of arsenite than A. caroliniana. Both strains effluxed more arsenate than arsenite, and the amount of As efflux was proportional to the amount of As accumulation. The potential of growing Azolla in paddy fields to reduce As transfer from soil and water to rice should be further evaluated.     

影响大型复杂机械钢结构系统可靠性的因素分析

以结构力学为理论依据,以有限单元法为基础,借助和参考有关有限元程序设计方法,按照结构系统可靠性失效模式分析的要求,采用编程语言研制成功基于有限元的可靠性分析软件RAPS(Reliability Analysis Program of Structure based on FEM),运用该软件对立体车库钢结构的可靠性进行具体的分析;对判定结构系统失效与否的失效准则进行讨论和分析,并针对立体车库钢结构,得出合理的失效准则和系统可靠度的计算方法;通过实例说明约界参数CAP、失效路径长度和系统复杂程度对失效准则和系统可靠度结果的影响。     

地下管线工程地震破坏等级划分标准

简要介绍了地下管线工程的地震破坏特点及类型，搜集整理了国内外地下管线工程地震破坏等级划分标准方面的有关标准、规定，对不同标准、规定进行了评述，在此基础上，以整个管网系统为评定对象，主要考虑管段破坏程度和单位长度管线破坏处数两个因素，将管网系统地震破坏细划为基本完好、轻微破坏、中等破坏、严重破坏及毁坏5个等级，给出了一套新的地下管线工程地震破坏等级划分标准。     

Comparative growth response of two varieties of <Emphasis Type="Italic">Vigna radiata</Emphasis> L. (var. PDM 54 and var. NM 1) grown on different tannery sludge applications: effects of treated wastewater and ground water used for irrigation

This study evaluates the possibility of using contaminated soil by treated tannery wastewater and the use of tannery sludge in agriculture. The plants of Vigna radiata var. PDM 54 grown on contaminated soil and irrigated with ground water have not shown the translocation of toxic metal (Cr) in the upper part. The biomass of the plant increased when irrigated with treated tannery wastewater compared to ground water, whereas no significant change was observed in chlorophyll and protein contents. In both the varieties (var. PDM 54 and var. NM 1) of V. radiata grown on tannery sludge amendments, the growth parameters exhibited a pronounced positive growth response up to 35% tannery sludge amendments compared with the plants grown on garden soil. Despite the Cr accumulation at lower amendments, no toxicity symptoms were observed in both the varieties of the plants. Higher amendments affected various growth parameters, NR activity, and carbohydrate content of the plants. The results suggest that the plants of V. radiata (var. PDM 54) may be grown on contaminated soil or lower sludge amendments and irrigated with ground water. No translocation of toxic metal Cr was found in the seeds of the plants grown in up to 25% tannery sludge. However, periodical monitoring is required before the consumption of seeds. Overall, the results showed that plant growth patterns were influenced to some extent by the level of soil contamination and the water used for irrigation.