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41.
42.
欧拉观测的大气扩散参数计算方法探析及应用 总被引:2,自引:0,他引:2
根据湍流扩散统计理论,讨论了利用欧拉观测的风速资料求解正态扩散模式中大气扩散参数的计算方法及其实现,并以海南洋埔开关区各稳定度大气扩散参数的计算为实例作了分析。 相似文献
43.
A case of fatal generalized dystrophic epidermolysis bullosa is described in a prematurely born female whose mother had strikingly elevated mid-trimester serum and amniotic fluid alpha-fetoprotein concentrations, a positive amniotic fluid acetylcholinesterase band, and negative serial ultrasound studies. This case lends further support to an association between autosomal recessive generalized dystrophic epidermolysis bullosa and increased levels of alpha-fetoprotein, positive amniotic fluid acetylcholines'terase, and normal ultrasound findings. 相似文献
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45.
In this paper we describe the use of five-colour fluorescence in situ hybridization for prenatal diagnosis of aneuploidy using uncultured amniotic fluid cells. The analysis is based on ratio mixing of dual-labelled probes and digital imaging for the detection and visualization of five different probes specific for the five target chromosomes, 13, 18, 21, X, and Y. A retrospective blind analysis of 30 coded uncultured amniotic fluid samples correctly detected fetal sex and five trisomy 21 cases. Multicolour fluorescence in situ hybridization used in this way allows rapid and simultaneous detection of the most frequent aneuploidies. 相似文献
46.
Rosa Cartolano Silvana Guerneri Roberto Fogliani Andrea Galimberti Umberto Nicolini 《黑龙江环境通报》1993,13(11):1057-1059
Trisomy 12 mosaicism diagnosed at 16 weeks' amniocentesis in a 42-year-old woman was not confirmed at 18 weeks' gestational age in amniotic fluid or fetal blood. Fetal skin biopsy performed at the same time did, however, allow the detection of trisomy 12 in 1 of 14 fibroblasts analysed. Fetal skin biopsy can be included within the diagnostic procedures to be performed when a level III mosaicism is found in the amniotic fluid. 相似文献
47.
催化裂化废水连续萃取脱酚研究 总被引:2,自引:0,他引:2
选用焦化粗柴油为萃取剂,在转盘萃取搭连续试验装置中,对催化裂化含酚废水进行脱酚预处理。结果表明,用焦化粗柴油做萃取剂,进水pH值为80~90不加调节;油水比(体积比)17∶1~21∶1;转盘转数1100r/min,酚萃取脱除率达到85%以上。萃取后的粗柴油依据现有工艺而进行加氢精制,不需进行再生等后处理。整个萃取工艺简单易行,能满足预处理的要求 相似文献
48.
大气飘尘对大鼠肺细胞的毒性比较 总被引:1,自引:0,他引:1
采用大白鼠气管内注入飘尘后分析肺灌洗液的方法,比较了上海不同地区飘尘对大鼠肺细胞的毒性;再运用毒性指数及效应指数,综合评价了上海市不同地区大气飘尘污染的状况。研究表明:从肺灌洗液的生化成份分析及细胞分类显示,以彭浦新村、隆昌路平凉路、康定路胶州路3个点的飘尘毒性较大。综合评价结果,彭浦新村的效应指数最大,以后依次为康定路胶州路、鲁班路斜土路、青云路、隆昌路平凉路等。 相似文献
49.
Reduction in serum requirement for culture of primary human amniotic fluid cells can be achieved by the addition of 10 growth-promoting factors to the nutrient medium. This supplemented medium preserves cell types normally found in amniotic fluid cell cultures supplemented with 20–30 per cent fetal bovine serum. The volume of amniotic fluid required to initiate culture can be as little as 1 ml. Amniotic fluid samples contaminated with red blood cells with no visible clot also grow well in the low serum medium. Cell-free amniotic fluid combined with equal parts of supplemented medium is useful in initiating cell culture. 相似文献
50.
The effects of reduced oxygen concentrations in the gas phase over the culture medium on colony formation and cell proliferation were investigated in high and low cell density primary and secondary cultures of amniotic fluid cells. Using two standard culture methods (25 cm2 plastic flasks and Leighton type tubes) a significantly reduced culture time was observed at high cell density for mass cultures by incubation within a low oxygen tension gas phase (2.5 per cent to 7.5 per cent O2) instead of conventional air (18 per cent O2). At low cell density colony formation was significantly enhanced in cultures grown at reduced oxygen tension. Using gas permeable membranes as support, lowering the oxygen tension from 7.5 per cent to 2.5 per cent yielded an increase in plating efficiency of cells from approximately 5 per cent to 25 per cent, whereas plating efficiency was less than 2 per cent for cells grown at ambient 18 per cent O2. It is suggested that low oxygen tension in the gas phase is an effective means of enhancing clonal growth in amniotic fluid cell cultures, thereby reducing both culture time and risk of culture failure. 相似文献