Exploration of the key functional proteins from an efficient cellulolytic microbial consortium using dilution-to-extinction approach

In the present study, the cellulose binding proteins(CBPs) secreted by a putative cellulolytic microbial consortium were isolated and purified by affinity digestion. The purified CBPs were subsequently separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis(SDS-PAGE). Using mass spectrometric analyses, eight CBPs were identified and annotated to be similar to known proteins secreted by Clostridium clariflavum DSM 19732 and Paenibacillus sp. W-61. In addition, in combination with dilution-to-extinction approach and zymogram analysis technique, CBPs 6(97 k Da) and 12(52 k Da) were confirmed to be the key functional proteins that influence cellulolytic activities. Moreover, structural domain analyses and enzymatic activity detection indicated that CBPs 6 and 12 contained glycoside hydrolase families(GH) 9 and 48 catalytic modules, which both revealed endoglucandase and xylanase activities. It was suggested that the coexistence of GH9 and GH48 catalytic domains present in these two proteins could synergistically promote the efficient degradation of cellulose.     

氯乙烯储罐的事故后果分析

氯乙烯具有毒性和易燃易爆性,如果泄漏至空气中,可能产生中毒或爆炸事故。笔者以某化工厂氯乙烯储罐为例,分析氯乙烯储罐可能发生的事故;对其主要事故危害,即中毒、蒸气云爆炸、扩展气体沸腾蒸气爆炸3种事故进行后果模型分析;计算出发生3种事故对人员伤亡和设备损坏造成的危害区域,并提出建议和对策。该研究结果可为同类企业进行安全管理提供科学依据和参考,有助于企业制定防范措施以及事故应急救援预案,从而减少人员伤亡及财产损失。     

如何完善沥青路面结构设计保证车辆行驶安全的探讨

沥青路面的病害直接威胁着公路通行车辆行驶安全,沥青路面的结构设计不合理是导致路面发生病害的重要原因之一。通过对高速公路沥青路面的主要病害成因从设计角度进行分析,提出应从完善路面设计基础数据的测试与确定方法、合理选择原材料、采用合适的矿料级配、完善配合比设计方法、合理选择沥青面层厚度、加强沥青面层层间及与基层的粘结等途径完善沥青路面结构设计,以进一步提高沥青路面的使用功能,减少道路交通安全事故的发生。     

中国实行洪水保险的可行性研究

在回顾洪水保险实施现状的基础上，指出了我国洪水保险与世界洪水保险的差距，根据我国洪水灾害的特点和我国经济体制的特点，提出了符合我国国情的洪水保险措施：建立洪水保险准备金是进行洪水保险的前提，洪水保险的特殊性决定了洪水保险不可能是个营利性的保险，因此要保证洪水保险的正常运行必须有社会和国家的扶持，洪水保险机构应按流域设立，中国洪水灾害发生的空间特点决定了洪水保险机构只能按流域设置，洪水灾害发生的时间特点决定了洪水保险的对象必须是固定资产，而不应该是流动资产，遭受洪水灾害影响最大的是农田，因此农田应该是洪水保险的对象，不应该是种植在农田里的农作物，另外可以通过农业保险对不同农作物进行保险，洪水保险需要洪水风险的分析和研究作技术支撑，没有科学的洪水风险分析，洪水保险很难顺利进行，加强与洪水保险有关的各方面法律法规的建立，在洪水保险法中明确界定洪水保险的保险对象，明确保险双方的义务和权利。     

DISCUSSION ON REGION RESOURCE ADVANTAGE OF THE WEST CHINA AND APPROACHES OF ITS INDUSTRIALIZATION DEVELOPMENT

With 70 .82 %ofthetotallandarea ,2 7.6 7%ofthetotalpopulationandrelativelyabundantnaturalresources,thevastwestChinaisextremelyimportantinstrategies.HowtomanagetheeconomicdevelopmentinthewestChinarapidlyhasarousedgreatattentionsinallwalksoflife .Theimportantpoliciesandmethodsaremainlydependingonthelarge scaleex plorationofnaturalresourcesanddevelopmentofrelevantindustries.However ,themisunder standingoflarge scalemineralexplorationsinthewestwillleadtoanoldroadofviciouscircula tion .Becauseofth…     

天然气输送管道泄漏事故危害定量分析

针对某市天然气利用工程中高压输送管道可能发生的天然气泄漏的危险性进行分析 ,求出了其最大的泄漏流量。通过高斯扩散模式定量计算在大气为中性条件下 ,天然气在大气中的轴向、径向和切向的扩散距离 ,从而计算出该天然气 -空气混合云团的体积。对该体积预混云团的火灾危害进行评估 ,计算形成火球的参数 ,用热辐射剂量准则计算其伤害半径。对该预混云团可能产生的爆轰后果 ,采用超压准则计算其对人员和建筑物的伤害半径。并用热辐射强度准则对可能产生稳态燃烧进行了危害半径的计算     

Realising local food policies: a comparison between Toronto and the Brussels-Capital Region’s stories through the lenses of reflexivity and co-learning

ABSTRACT

This paper revisits the development of Toronto and Brussels’ local food policies by analysing reflexivity and co-learning as important dimensions within a Hybrid Governance Approach (HGA); it approaches the interaction between four forms of governance (bottom-up, networked, state, market-led) and the tensions between them as hybrid dynamics. Within this approach, reflexivity refers to the positionality of agents, i.e. to the ways local food actors embody as well as reflect on and reconsider their principles and practices through time. Closely related to reflexivity, co-learning involves agential interactions to co-construct enabling food policy delivery systems. The HGA is mobilised to understand the ways in which reflexive capacities, as well as co-learning, take place in the two cases and how they lay the basis of particular modes of (de)institutionalisation. Learning from the two cases’ trajectories, this paper highlights: (a) the role of key governance tensions as triggers as well as breeding grounds for reflexivity and co-learning outcomes; (b) the challenges of food movement actors to surmount or valorise key tensions in order to build accountable modes of food policy delivery through time; (c) the struggles to build legitimacy and accountability in local food movements through the development of bottom-linked organisations and governance modes.     

城市污水再生利用途径分析与利用量预测

水资源匮乏与水环境污染是制约经济社会发展的主要瓶颈,提高城市污水资源化水平是解决这一问题的重要途径.文章结合〈城市污水再生利用分类标准〉（GB/T 18919-2002）,深入分析包头市城市污水处理与再生利用现状及存在问题,提出包头市再生水近期应主要回用于城市杂用、工业和景观用水,再生水回用率可达到50%以上,为解决城市污水再生利用提供参考.     

Triad, 污染场地管理的新模式

污染场地的调查和修复往往是个耗时很长、成本很高的过程.传统的多阶段的场地管理模式是造成这一状况的重要原因之一.随着场地管理经验的增加和新技术的出现,美国形成了一种能大大缩短项目周期、显著降低成本的场地管理新模式:Triad模式.本文系统阐述了Triad模式的3个要素,即系统的项目计划,动态的工作策略和实时的测量技术,结...     

北京市大气污染物中可吸入颗粒物（PM10）造成的健康损失研究——人力资本法实例研究

摘要：本文使用北京市2000-2008年空气中可吸入颗粒物（PM10）的浓度变化数据描述北京市大气污染程度的变化趋势,并应用改进的人力资本法,即使用流行病学关于疾病负担的计量方法——YPLL潜在寿命损失年法来评价（PM10）对北京市居民健康造成的经济损失。结果表明：北京市大气污染物中可吸入颗粒物在2008年明显减少,低于空气中最高容许浓度,以脑血管疾病和呼吸系统疾病对其健康效益进行估算,可得出其产生的经济效益为44—4,8亿元,在北京市环保投入收益中占有一定比重。