化学纤维栅湿式过滤除尘的实验研究

化学纤维栅湿式除尘是一种复合机理的新型湿式过滤除尘技术,其过滤风速为2～10m/s,阻力仅为200～500Pa,除尘效率达到99.62％,能很好解决矿山溜井卸矿产生的粉尘,使溜井含尘气流经净化后达到新鲜风流卫生标准。     

一种控制粮食工厂溜管噪声的简易措施

溜管噪声产生的原因是物料颗粒与溜管壁及物料颗粒之间相互撞击的结果,提出了降低噪声的简单措施,在溜管中安装缓冲箱和降速板可取得较好的降噪效果。     

计算机辅助确定外因火灾位置方法

系统介绍用计算机辅助确定矿井外因火灾位置方法的原理,给出该方法的具体步骤,剖析其定性分析、定量分析和定量分析结果评价三大步骤中的各技术关键,并借助一个实例验证了该方法实用的可行性     

安徽沿江地区的区域发展特点与开发对策

安徽沿江地区虽然交通网络初具规模,城市专业化分工明显经济发展水平较低,属长江干流地区的低谷地带。另外,整个沿江地区应发挥水运与矿产资源的双重优势,促使建材,钢铁,石经等临江工业进一步开并予以合理布局。     

环境风险评价研究进展

重大环境污染事故常伴有人员伤亡,给社会、经济、人群健康和环境带来巨大破坏,环境风险评价是预防环境污染事故并提供有效应急措施的必要工作.对环境风险评价发展的三个阶段:健康风险评价、事故风险评价和综合风险评价进行了总结,并分析其研究进展、评价方法和研究不足之处,最后探讨了环境风险评价与环境风险管理的关系.结果表明,在未来几十年内,区域环境风险、长期环境风险、不确定性处理和环境毒理学、环境流行病学等方面将成为环境风险评价的重点研究领域.     

Mosaicism for trisomy 12: Four cases with varying outcomes

Trisomy 12 observed in chorionic villus sampling (CVS) may reflect generalized mosaicism or indicate mosaicism confined to only the placenta. In this report, four cases of trisomy 12 observed in CVS or cultured placental biopsies with varying outcomes are presented. Seven dinucleotide repeat polymorphisms for chromosome 12 were used to determine the chromosome 12 origins in the fetus or child and to delineate the mechanism(s) that gave rise to the trisomy. In two cases (cases A and C), the mosaicism was confined to the placenta, resulting in normal liveborns. Although, in one case, the molecular results suggested an apparent duplication of one paternal chromosome 12 in the placenta, normal biparental inheritance was found in the diploid fetal cell line in both cases. In two other cases (cases B and D), trisomy 12 was observed in both extraembryonic and fetal tissues. In one of these pregnancies, a child was born by Caesarean section at 37 weeks because of intrauterine growth retardation and oligohydramnios, and resulted in neonatal death. Molecular markers and fluorescence in situ hybridization (FISH) revealed low-level trisomy 12 mosaicism in the spleen. In the fourth case, fetal abnormalities were detected on ultrasound and low-level trisomy 12 mosaicism was observed in amniotic fluid cells using conventional cytogenetics and FISH. Molecular markers revealed a maternal meiosis I non-disjunction of chromosome 12 in DNA from a cultured placental biopsy. Although predicting the outcomes of pregnancies involving confined placental mosaicism remains difficult, molecular techniques are valuable tools for distinguishing uniparental from biparental disomy and mechanisms of mosaicism.     

光氧化吸附矿井水中微量有机物试验研究

针对目前煤矿矿井水资源化过程中存在有机污染物的问题，考察了采用紫外光氧化分解的机理及影响因素。结果表明：紫外光对矿井水中有机物（主要是乳化油）有一定的氧化分解能力，能有效地促使有机物大分子破裂，有利于活性炭的吸附处理。在合理的工艺条件下，可使水中有机物去除率达到95%以上。     

Evaluation of X,Y, 18, and 13/21 alpha satellite DNA probes for interphase cytogenetic analysis of uncultured amniocytes by fluorescence in situ hybridization

The major aneuploidies diagnosed prenatally involve the autosomes 13, 18, and 21, and sex chromosomes. Fluorescence in situ hybridization (FISH) allows rapid analysis of chromosome copy number in interphase cells. This prospective study evaluated the use of four commercially available centromeric DNA probes (DXZ1, DYZ1, D18Z1, and D13Z1/D21Z1) for direct analysis of uncultured amniocytes. One hundred and sixteen amniotic fluid samples were analysed by FISH and standard cytogenetics. This evaluation demonstrated that FISH with, X, Y, and 18 alpha satellite DNA probes could accurately and rapidly detect aneuploidies involving these chromosomes and could be used in any prenatal clinical laboratory. In contrast, the 13/21 alpha satellite DNA probe hybridizing both chromosomes 13 and 21 was unreliable for prenatal diagnosis in uncultured amniocytes.     

Prenatal detection of trisomy 21 in uncultured amniocytes by fluorescence in situ hybridization: A prospective study

A prospective study was undertaken to evaluate the use of fluorescence in situ hybridization (FISH) for the detection of trisomy 21 in interphase nuclei of uncultured amniotic fluid cells. Five hundred cases were analysed in situ and classified as normal or abnormal; the results were subsequently checked against the cytogenetic findings. Four hundred and ninety-three were correctly identified as normal with an 86·6 per cent average frequency of scored nuclei exhibiting two signals; six cases were correctly identified as trisomic for chromosome 21 with 81·7 per cent of scored nuclei exhibiting three signals; and one abnormal case involving an unbalanced chromosome 21·21 translocation was falsely scored as normal due to poor hybridization/detection efficiency. The method has been substantially improved and simplified so that it is suitable for the rapid detection of trisomy 21. As aneuploidy detection in interphase does not identify structural chromosome aberrations, it is not a substitute for fetal chromosome analysis.     

Molecular cytogenetic analysis of term placentae suspected of mosaicism using fluorescence in situ hybridization

In first-trimester chorionic villus sampling (CVS) for prenatal diagnosis, abnormal chromosomal findings, such as mosaicism, trisomies, or suspect abnormal karyotypes, are found more frequently than at amniocentesis. The fact that these chromosomal abnormalities do not always reflect the fetal karyotype but may be restricted to the placenta is a major problem in diagnosis and counselling. In this paper we present the results of fluorescence in situ hybridization (FISH) studies on interphase nuclei of three term placentae investigated because of false-positive findings at first-trimester CVS. The chorionic villi of the first case showed a mosaic chromosome pattern involving a trisomy 10 cell line and a normal cell line, those of the second case a total trisomy 8 cell line, while in the third case a complete monosomy X was found. Follow-up amniocentesis in each of these three cases revealed a normal karyotype. By using FISH, we were able to confirm the presence of the aberrant cell lines, which were all confined to one part of the placenta. FISH on interphase nuclei allows the investigation of large numbers of cells for the existence of numerical chromosome aberrations in a quick and reliable way.