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11.
为了探究淡水贝类背角无齿蚌鳃细胞的原代培养途径,并阐释其在评价水体Cd2+毒性效应上的潜力,本研究比较了不同酶分解方法(链霉蛋白酶、胰蛋白酶)的鳃细胞存活率差异,并分析了L-15培养基中不同血清浓度(10% FBS、20% FBS)对鳃细胞存活率的影响,细胞置于20℃生化培养箱中培养.进而根据Cd2+对鳃细胞的LC25值设定0.0625、0.125、0.25、0.5和1.0 mg·L-1 5个Cd2+理论浓度梯度,对原代培养的鳃细胞进行24 h暴露,分析了细胞活力、总超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和酸性磷酸酶(AcP)的变化特征.结果表明:0.025%链霉蛋白酶在4℃分解16 h的鳃细胞存活率为98.2%±0.2%,显著高于0.25%胰蛋白酶在26℃分解30 min的89.4%±3.5%鳃细胞存活率(p<0.05);L15培养基加入10% FBS的细胞存活率总体显著高于添加20% FBS的细胞存活率(p<0.05).在上述较佳的分解和血清浓度组合条件下,细胞培养120 h后,其存活率仍高达90.1%±4.7%.鳃细胞活力随着Cd2+浓度的增加而降低,两者之间呈显著的线性负相关(p<0.05);随着Cd2+浓度的增加,SOD和AcP含量总体增加,而CAT含量呈现出"诱导-抑制"的变化趋势.本研究初步建立了较为适宜的背角无齿蚌鳃细胞的原代培养方法,并揭示了其原代培养鳃细胞的细胞活力及SOD、CAT、AcP水平,具有作为评价水环境Cd2+毒性/污染的生物指标的潜力. 相似文献
12.
城市物流形成绿色化发展趋势的主要动因在于汽车运输业的迅速发展及其给城市环境与人们生活质量所造成的巨大影响上.二战以后,汽车运输业的迅速发展和在给社会提供迅速、便捷与准确服务的同时,也给城市的环境造成了严重的负面影响;尤其是由于城市路网和货物运输中心等基础设施既落后且缺乏科学的规划,无形中更加剧了城市物流与城市环境保护之间的矛盾.为实现城市物流的绿色化发展,实践中就有必要合理规划城市物流系统、通过多式联运以尽量降低卡车运输量的比例、积极应用信息技术以推进和实现物流管理的智能化、发展第三方物流实现共同配送. 相似文献
13.
Dr. O. W. Jones Nolan E. Penn Stephen Shuchter Claire A. Stafford Teri Richards Colleen Kernahan Jennifer Gutierrez Patricia Cherkin Sylvia Reinsch Barbara Dixson 《黑龙江环境通报》1984,4(4):249-256
This article reports the results of a retrospective study designed to examine the responses of couples to genetic amniocentesis and subsequent therapeutic abortions due to birth defects. Fourteen women and 12 men were interviewed by experienced interviewers using a structured format designed by the authors, and each interview was audiotaped for later rating. The 5 raters (all women) were instructed to independently rate each interview using forms designed by the authors to elicit information about many aspects of the participant's individual responses as well as perceptions of spouse's responses to the process of pregnancy, amniocentesis, therapeutic abortion, and sequelae. Ratings of all 5 raters were conjoined and an homogeneous narrative was constructed for each interview. Results indicate, in general, that the respondent couples coped well with this experience. In fact 70 per cent of the respondent couples described their marital relationships as becoming closer as a result of their experience. Only a few participants reported long-term deleterious effects. Most couples coped by relying on relatives, friends, and occasionally, professional counsellors. In addition, most participants in this study suggested ways to improve the medical and psychological aspects of this experience. 相似文献
14.
本文回顾了灾害经济学的发展历程,分析了灾害经济学的理论框架及与其他学科的关系,从经济学的角度阐述了建立这一学科的必要性,并探讨了建立这一学科的基础条件.全文从经济学的角度阐述了建立这一学科的必要性.并探讨了建立这一学科所要研究的理论知识. 相似文献
15.
16.
Oliveira Amauri P. Bornstein Robert D. Soares Jacyra 《Water, Air, & Soil Pollution: Focus》2003,3(5-6):3-15
The major topographic, mesoscale, and urban influences on the wind patterns of the City of São Paulo are characterized using one year of surface wind velocity data observed at 11 surface stations within its urban limits. The data was used to study the diurnal and annual variations of wind velocity and horizontal wind divergence within the city. Results showed that the circulation over the investigated area is dominated by three major factors: sea breeze; mountain-valley circulations; and urban effects, such as roughness, building-barrier, and urban heat island. The sea breeze was found to be the dominant feature of the monthly-averaged diurnal variation of São Paulo surface winds during the eight warmest months of the year. The sea breeze front induces a velocity minimum at the time of its passage and a post-frontal afternoon velocity maximum. Mountain-valley thermal effects on the flow can be seen in the temporal divergence/convergence patterns. These thermal effects tend to be more important during colder months, at night, and when the wind velocities are low. Nighttime downslope convergent flows are present over the city during winter and spring and daytime upslope divergent flows are present over the city during summer months. 相似文献
17.
Paul V Bolstad Wayne T Swank 《Journal of the American Water Resources Association》1997,33(3):519-533
ABSTRACT: Water quality variables were sampled over 109 weeks along Coweeta Creek, a fifth-order stream located in the Appalachian mountains of western North Carolina. The purpose of this study was to observe any changes in water quality, over a range of flow conditions, with concomitant downstream changes in the mix of landuses. Variables sampled include pH, HCO32?, conductivity, NO3??-N, NH4+-N, PO43?-P, C1?-, Na, K, Ca2+, Mg2+, SO42?, 5iO2, turbidity, temperature, dissolved oxygen, total and fecal coliform, and focal streptococcus. Landcover/landuse was interpreted from 1:20,000 aerial photographs and entered in a GIS, along with information on total and paved road length, building location and density, catchment boundaries, hydrography, and slope. Linear regressions were performed to relate basin and near-stream landscape variables to water quality. Consistent, cumulative, downstream changes in water quality variables were observed along Coweeta Creek, concomitant with downstream, human-caused changes in landuse. Furthermore, larger downstream changes in water quality variables were observed during stormflow when compared to baseflow, suggesting cumulative impacts due to landscape alteration under study conditions were much greater during storm events. Although most water quality regulations, legislation, and sampling are promulgated for baseflow conditions, this work indicates they should also consider the cumulative impacts of physical, chemical, and biological water quality during stormflow. 相似文献
18.
Threshold values for domino effects caused by blast wave interaction with process equipment 总被引:6,自引:0,他引:6
Valerio Cozzani Ernesto Salzano 《Journal of Loss Prevention in the Process Industries》2004,17(6):437-447
The present study focuses on the definition and assessment of overpressure threshold values for the damage to equipment caused by blast waves originated by primary accidental scenarios. A revision of literature data and of the available damage probability models was carried out. Threshold values were proposed for different categories of process equipment, taking into account either damage levels or release intensities following the loss of containment. Specific threshold values for domino effect were also proposed. 相似文献
19.
Vicente L. Lopes H. Evan Canfield 《Journal of the American Water Resources Association》2004,40(2):311-319
ABSTRACT: This paper evaluates the effects of watershed geometric representation (i.e., plane and channel representation) on runoff and sediment yield simulations in a semiarid rangeland watershed. A process based, spatially distributed runoff erosion model (KINEROS2) was used to explore four spatial representations of a 4.4 ha experimental watershed. The most complex representation included all 96 channel elements identifiable in the field. The least complex representation contained only five channel elements. It was concluded that oversimplified watershed representations greatly influence runoff and sediment yield simulations by inducing excessive infiltration on hillslopes and distorting runoff patterns and sediment fluxes. Runoff and sediment yield decrease systematically with decreasing complexity in watershed representation. However, less complex representations had less impact on runoff and sediment‐yield simulations for small rainfall events. This study concludes that the selection of the appropriate level of watershed representation can have important theoretical and practical implications on runoff and sediment yield modeling in semiarid environments. 相似文献
20.
Maie Bachmann Maksim Säkki Jaan Kalda Jaanus Lass Viiu Tuulik Hiie Hinrikus 《The Environmentalist》2005,25(2-4):165-171
Summary This study focuses on discrimination of changes, produced by low-level microwave exposure in intensity and time variability
of the human EEG at rest. The power spectral density (PSD) method and nonlinear scaling analysis of the length distribution
of low variability periods (LDLVP) were selected for analysis of the EEG signal. During the study, 19 healthy volunteers were
exposed to a microwave (450 MHz) of 217 Hz frequency on-off modulation. The field power density at the scalp was 0.16 mW/cm2. The experimental protocol consisted of ten cycles of repetitive microwave exposure. Signals from frontal, temporal, parietal
and occipital EEG channels on EEG theta, alpha and beta rhythms were analysed. Exposure to microwave causes average increase
of EEG activity. LDLVP analysis discriminated significant effect in time variability for 2 subjects (11%). PSD method detected
significant changes in intensity for 4 subjects (21%). The effect of low-level microwave exposure is stronger on EEG beta
rhythm in temporal and parietal regions of the human brain. 相似文献