抗生素最小抑制浓度法(MICs)评估环境大肠杆菌抗生素抗性

2015年世界卫生组织将抗生素的滥用列为21世纪最大的挑战之一,全球范围内抗生素抗药性的散播已严重威胁人类的健康。如何检测环境中的细菌抗药性,并有效评估抗药性感染的风险,是环境微生物研究的一项重要课题。本文通过改进的培养基微量稀释(broth micro-dilution)法,确定了2个地区(中国成都和美国夏威夷)不同环境来源(天然水系和市政污水)的大肠杆菌的抗生素最小抑制浓度(minimum inhibitory concentrations,MICs)。计算所得的MIC分位数(MIC50和MIC90)、菌体抗性百分比及多抗药性指数(multiple antibiotic resistance indexes,MARIs)显示两地不同环境区划的抗生素抗性存在明显的差异。天然水系(成都锦江)中的抗生素抗性是随时间可变的,与当地的降雨事件相关。环境菌株的抗药性模式通过聚类和非度量多维测度(non-metric multidimensional scaling,NMDS)进行分析。广谱β-内酰胺酶基因筛查显示出抗性基因与抗性表型之间的正相关性。结合现有的两地抗生素的使用数据讨论了两地环境抗生素抗性与当地人类活动及抗生素的使用实践之间的紧密联系。采集环境菌株抗生素MIC数据的实验及数据分析方法实现了环境抗药性的跨时空对比,为规范抗生素的区域性使用提供了指导作用。     

From NIMBY to enlightened resistance: a framework proposal to decrypt land-use disputes based on a landfill opposition case in France

Not In My Back Yard (NIMBY) supporters are presented as citizens in opposition to local developments due to their spatial proximity. However, these conflicts have proven much more complex than the NIMBY concept can explain. The objective of this paper is to provide a framework to facilitate the understanding of opposition movements and how they can affect society at large, triggering social change. The conceptual framework is applied to a case of local opposition to a landfill project in Essonne, France. Through the analysis of the structure of the opposition movement and its changes over time, the author shows how it can evolve into a social movement that enriches democracy through the constitution of four types of capital: social, scientific, patrimonial and political. The author argues that scientific and patrimonial capitals allow social capital to evolve into political capital. The shift from being a self-interest to a civic interest movement is called enlightened resistance, which reveals local public interest, called territorial interests. When studying environmental controversies, the author emphasises the importance of recognizing the evolution through time of (1) social landscape, (2) different types of legitimate knowledge, (3) the role of place attachment, and (4) the political dimension of identities.     

Tetracycline resistance in semi-arid agricultural soils under long-term swine effluent application

Annually, millions pounds of antibiotics are released unmetabolized into environment along with animal wastes. Accumulation of antibiotics in soils could potentially induce the persistence of antibiotic resistant bacteria. Antibiotics such as tetracyclines and tetracycline-resistant bacteria have been previously detected in fields fertilized with animal manure. However, little is known about the accumulation of tetracyclines and the development of tetracycline resistance in semi-arid soils. Here we demonstrate that continuous land application with swine effluent, containing trace amounts of chlortetracycline, does not necessarily induce tetracycline resistance in soil bacteria. Based on the testing of more than 3,000 bacteria isolated from the amended soils, we found no significant increase in the occurrence and level of chlortetracycline resistant bacteria in soils after 15 years of continuous swine effluent fertilization. To account for a possible transfer of tetracycline-resistant bacteria originated from the swine effluent to soils, we analyzed two commonly found tetracycline resistant genes, tet(O) and tet(M), in the swine effluent and fertilized soils. Both genes were present in the swine effluent, however, they were not detectable in soils applied with swine effluent. Our data demonstrate that agronomic application of manure from antibiotic treated swine effluent does not necessarily result in the development of antibiotic bacterial resistance in soils. Apparently, concentrations of chlortetracycline present in manure are not significant enough to induce the development of antibiotic bacterial resistance.     

Reduction of U(VI) by the deep subsurface bacterium, Thermus scotoductus SA-01, and the involvement of the ABC transporter protein

In this study we investigated the effect of uranium on the growth of the bacterium Thermus scotoductus strain SA-01 as well as the whole cell U(VI) reduction capabilities of the organism. Also, site-directed mutagenesis confirmed the identity of a protein capable of a possible alternative mechanism of U(VI) reduction. SA-01 can grow aerobically in up to 1.25 mM uranium and has the capability to reduce low levels of U(VI) in under 20 h. TEM analysis performed on cells exposed to uranium showed extracellular and membrane-bound accumulation of uranium. The reductase-like protein was surprisingly identified as a peptide ABC transporter, peptide-binding protein. This study showcases the concept of protein promiscuity, where this protein with a distinct function in situ can also have the unintended function of a reactant for the reduction of U(VI).     

高温后足尺有粘结预应力混凝土梁板力学性能研究

针对足尺有粘结预应力混凝土梁板高温后的抗弯承栽力开展了非线性计算分析。结果表明,综合配筋指标、有效预应力对高温后极限弯矩与常温下极限弯矩比值M_u~A/M_u的影响不大,预应力度和预应力筋的保护层厚度对M_u~A/M_u影响显著。高宽比近似相同的有粘结预应力混凝土梁,当第一排钢筋的保护层厚度相同时,受火时间为3 h,高度为700 mm的梁高温后截面承栽力下降幅度最大约为30%,高度为4500 mm的梁高温后截面承栽力下降幅度不到10%,高温后大尺度梁的力学性能优于小尺度梁。给出了考虑预应力筋保护层厚度和预应力度影响的有粘结预应力混凝土板M_u~A/M_u的计算公式,以及综合考虑预应力筋、非预应力筋合力点到梁底距离和预应力筋梁侧保护层厚度影响的有粘结预应力混凝土梁M_u~A/M_u的计算公式。     

高浓度铬污染土壤水浸泡与电动修复联合处理实验

对含铬量为2 142 mg/kg的铬污染土壤首先使用蒸馏水进行6轮浸泡试验,然后再进行电动修试验。土壤经过浸泡、通电24 h和72 h处理后总铬的去除率分别为:47%、61%和70%,表明预浸泡可以显著降低电动修复的负荷从而降低整个土壤修复费用。电动修复过程中对土壤两端的温度进行了测量以间接反映土壤电阻的分布和变化情况。结果显示土壤电阻逐渐上升,从阴极端开始逐渐过渡到阳极端,60 h之后达到最高值并保持稳定。电动修复的电流效率随土壤中铬浓度的降低而下降。此外,浸泡和电动修复均导致整个土壤和各断面铬浓度分布不均匀性增大。     

Ciprofloxacin-sensitive and ciprofloxacin-resistant Campylobacter jejuni are equally susceptible to natural orange oil-based antimicrobials

A total of 10 ciprofloxacin-sensitive (ciprofloxacin minimum inhibitory concentration, MIC < 0.5 μ g/ml) and 10 ciprofloxacin-resistant (MIC 16 to 32 μ g/ml) presumptive C. jejuni were further characterized and evaluated for their inhibition by natural orange oil fractions. Partial species identification was performed by using a hippuricase gene-based polymerase chain reaction (PCR) assay. One of the isolates appeared to be atypical and failed to hydrolyze hippurate. Of the ciprofloxacin-resistant C. jejuni isolates tested, six were found to have their quinolone resistance determined by a C → T mutation in codon 86 of gyrA. Both groups of ciprofloxacin-sensitive and –resistant C. jejuni isolates were most susceptible to cold-pressed terpeneless Valencia orange oil (C4) which yielded inhibition zones from 44.0 ± 1.4 to 80 ± 0.0 mm. Less inhibitory responses were recorded for 5-fold concentrated Valencia orange oil (C3) and distilled d-limonene (C7) which exerted similar effects on both ciprofloxacin-sensitive and –resistant C. jejuni isolates. In general, ciprofloxacin-resistant and –sensitive C. jejuni isolates were equally susceptible to the respective orange oil fractions.     

浅谈影响烧结机头电除尘器效率的主要因素

针对烧结机头烟气、烟尘的特点,对影响机头电除尘器运行效率的主要因素进行了分析,并提出了一些应付不利因素的对策,为提高烧结机机头除尘器除尘效率和使用寿命,进一步加强和改善除尘器运行效果,实现达标排放提供了理论依据及技术方案。     

Biomonitoring marine habitats in reference to antibiotic resistant bacteria and ampicillin resistance determinants from oviductal fluid of the nesting green sea turtle, Chelonia mydas

During the egg-laying process, oviductal fluid was collected using a non-invasive procedure from the cloacal vent of the green turtles. Forty-two independent isolates of antibiotic-resistant bacteria from 11 genera were obtained from 20 turtles during nesting. The dominant isolate was Citrobacter (52.4%), followed by Pseudomonas, Proteus, Enterobacter, Salmonella, Escherichia coli, Shigella, Edwardsiella, Morganella, Providencia and Arcomobacter. Most of the isolates were resistant to ampicillin. Ampicillin-resistant isolates showed variations in their resistance for the following classes of β-lactamases: extended-spectrum β-lactamases (EBSLs), AmpC type β-lactamases C (AmpC), and screen-positive β-lactamase. None of the isolates produced metallo β-lactamase. Some ampicillin-resistant genes were detected by multiplex polymerase chain reaction (PCR) only. Inhibitor based test (IBT) categorized some isolates as AmpC β-lactamase producers. β-Lactamase genes were detected from a few strains. The sequencing of those genes revealed the presence of cephamycinase (CMY) and AmpC β-lactamases. The oviductal fluid was used in this study as a source of bacterial antibiotic-resistant determinants for biomonitoring marine turtles exposed to contaminated effluents. This data can be of value in understanding the decline of this endangered species as a result of exposure to marine pollution which is threatening their survival.     

The microbiota of an unpolluted calcareous soil faces up chlorophenols: Evidences of resistant strains with potential for bioremediation

To highlight the effects of a variety of chlorophenols (CP) in relation to the response of an indigenous bacterial community, an agricultural Mediterranean calcareous soil has been studied in microcosms incubated under controlled laboratory conditions. Soil samples were artificially polluted with 2-monochlorophenol (MCP), 2,4,6-trichlorophenol (TCP) and pentachlorophenol (PCP), at concentrations ranging from 0.1 up to 5000 mg kg⁻¹. Both activity and composition of the microbial community were assessed during several weeks, respectively, by respirometric methods and PCR-DGGE analysis of extracted DNA and RNA. Significant decreases in soil respirometric values and changes in the bacterial community composition were observed at concentrations above 1000 mg kg⁻¹ MCP and TCP, and above 100 mg kg⁻¹ PCP. However, the persistence of several active bacterial populations in soil microcosms contaminated with high concentration of CP, as indicated by DGGE fingerprints, suggested the capacity of these native bacteria to survive in the presence of the pollutants, even without a previous adaptation or contact with them.The isolation of potential CP degraders was attempted by culture plating from microcosms incubated with high CP concentrations. Twenty-three different isolates were screened for their resistance to TCP and PCP. The most resistant isolates were identified as Kocuria palustris, Lysobacter gummosus, Bacillus sp. and Pseudomonas putida, according to 16S rRNA gene homology. In addition, these four isolates also showed the capacity to reduce the concentration of TCP and PCP from 15% to 30% after 5 d of incubation in laboratory assays (initial pollutant concentration of 50 mg L⁻¹). Isolate ITP29, which could be a novel species of Bacillus, has been revealed as the first known member in this bacterial group with potential for CP bioremediation applications, usually wide-spread in the soil natural communities, which has not been reported to date as a CP degrader.