13种花卉植物受硫氧化物混合气体污染的伤害症状及其抗性表现

通过熏气的方法,比较了在硫氧化物混合污染气体的作用下,木本花卉（月季Rosa chinensis,杜鹃Rhododendron simsii,一品红Euphorbia pulcherrima,茉莉花Jasminum sambac）,一、二年生花卉（一串红Salvia splendens,凤仙花Impatiens balsamina）,宿根花卉（君子兰Clivia miniata）,室内观叶植物（文竹Asparagus setacens,吊兰Chlorophytum comosum,沿阶草Ophiopogon bodinieri,朱蕉Cordyline fruticosa,吊竹梅Zebrina pendula,白斑万年青Dieffenbachia bowmannii）中13种花卉植物的叶片伤害症状及植物抗性表现,并对植物受害后的恢复情况进行了追踪观察。发现在同种污染气体的作用下,不同种类的植物表现出不同的伤害症状。植株不同叶龄的叶片对污染气体的敏感性存在一定的差异,幼龄叶不一定比中老龄叶敏感性强。当污染浓度不超过一定阈值时,花卉植物都有不同程度的恢复能力。利用“花卉植物叶片伤害评价系统”对试验植物叶片伤害症状进行了评价,依据评价结果,对试验植物进行了抗性分级。为保证对植物抗性等级划分的准确性,建议制定统一的熏气浓度标准和评价标准。     

获取NEBS认证的“3P”阶段

NEBS认证,是电信网络设备进入北美地区电信网络的通行证。本文把获取NEBS认证的全过程划分为三个阶段,即准备阶段、设计阶段和实施阶段,并论述了各阶段的关键要点。首先,在准备阶段对NEBS进行了介绍,简要分析了NEBS认证的主要标准,同时指出运营商的特殊要求的重要性。接着分析了在设计阶段应该注意的技术点,如空间尺寸要求、电源输入、接地、防火、地震等。最后,讨论了认证测试阶段面临的困难,并强调了认证前摸底测试的重要性及必须具备足够的耐心。     

化学防护服的氢氧化钠渗透性能离子色谱检测方法的研究

渗透性能是化学防护服最重要的防护性能指标之一.本文利用PTC200型渗透测试池、蠕动泵和离子色谱仪等装置,研究并建立了NaOH溶液的渗透性能离子色谱仪的检测方法.结果表明,利用该装置可以方便准确地实现服装面料NaOH溶液渗透性能测试,是一种具有重要应用价值的检测方法.     

A long term study of the desiccation resistance of Drosophila melanogaster and D. simulans from the Mexican nuclear power plant “Laguna Verde”

The generation of electricity through the use of radioactive material at the nuclear power plant is inevitably associated with the production of wastes, some of which have potential impact on the biosphere. The objective of the present investigation was to provide information for evaluating the presumed impact of the Mexican Nuclear Power Plant “Laguna Verde" on the natural populations. Two sibling species that live in the immediate vicinity, Drosophila melanogaster and D. simulans have been studied for several traits in a long term study. The present study describes results for the desiccation resistance (DR) trait obtained during the period from 1995 to 2002. Flies were collected at two sites, one near the reactors and another farther away. The data obtained confirmed that D. melanogaster had higher DR values than D. simulans at both sites. The analysis of the results obtained from both species of the site closer to the reactor indicated that the values of the DR in the operational stage did not change, compared with those in the preoperational stage previously analyzed. Therefore, the significant differences found between the monitored sites did not seem to be associated to the operation of the reactors. L. Levine Died June 14th 2006     

基于GIS的高原湖泊流域生态安全格局构建及优化研究——以星云湖流域为例

以星云湖流域自然人文环境特征、主要生态环境问题为出发点,结合景观生态学原理方法、熵值法和GIS空间分析技术,实现星云湖流域生态安全的空间分异格局及优化。选取海拔高程、坡度、土壤侵蚀强度、地质灾害易发性、土地覆被(利用方式和植被覆盖度)、人口密度、距自然保护区距离、距离建设用地和距水源距离等因素为阻力因子叠加生成综合阻力要素,根据GIS空间成本距离分析原理,引用最小累积阻力模型识别构建流域生态廊道和生态节点,进一步规划优化流域生态功能网络的结构和功能。研究表明:(1)从各安全等级面积比例和平均生态安全指数角度,星云湖流域中度安全占流域总面积的45.81%,高度安全为25.24%,较低安全为21.05%,不安全占7.9%,平均生态安全指数为2.95,流域生态安全总体处于中度安全,生态系统健康一般,但部分地区生态安全性较低,主要分布在南部、东部和北部地区,应重视这些区域的生态保护和建设;同时,坡度、水土流失因子和水资源(自然环境要素)、土地利用格局和距离建设用地(人类活动干扰要素)等因素是流域生态安全格局形成的主要因子;(2)识别规划了中枢生态源、核心型生态源斑块、一般生态源、多种级别的生态廊道和生态节点或战略点等构成的具有结构性和层次性的流域生态安全网络格局,对不同层次和结构的生态源、生态廊道和生态节点或战略点进行针对性建设保护,将有效提升流域生态系统的功能价值性和系统的完整性,进而加强流域生态稳定安全。     

离子液体[BMIm][PF6]增强抗性质粒RP4介导的接合转移基因mRNA的表达水平

环境中广泛存在的抗生素和抗生素抗性基因会导致很严重的人类健康风险。在我们前期研究中发现,离子液体1-丁基-3-甲基咪唑六氟磷酸盐([BMIm][PF6])作为环境选择性压力可以促进抗生素抗性基因的水平转移。本研究以E.coli DH5α(RP4)和同属的E.coli HB101,以及E.coli DH5α(RP4)和跨属的Salmonella enterica之间的纯菌接合转移体系为考察对象,从mRNA基因表达调控水平角度阐明离子液体[BMIm][PF6](0.001~2.5 g·L-1)影响质粒RP4接合转移的机理。结果表明,离子液体[BMIm][PF6]通过抑制基因kor A,kor B和trb A的mRNA表达水平来提高接合和跨膜转运基因trb Bp和trf Ap的表达;增强水平转移基因tra F的mRNA表达水平,并通过增强负调控基因kil A和kil B的mRNA表达水平抑制质粒的垂直传递过程;通过抑制基因trb K的mRNA表达水平降低接合转移体系的排斥效果,从而促进质粒RP4的接合转移。该结果有助于为抗生素抗性基因在环境中水平转移扩散的机理研究提供理论依据。     

Relationship between antibiotic residues and occurrence of resistant bacteria in Nile tilapia (Oreochromisniloticus) cultured in cage-farm

The aim of this study was to investigate the relationship between antibiotic residues found in the muscle of cage-farm-raised Nile tilapia (Oreochromisniloticus), the occurrence of resistant bacteria, and the sanitary practices adopted by farmers in Ilha Solteira reservoir, Brazil. Nine fish (three small fish, 40–200 g; three medium-sized fish, 200–500 g; and three large fish, 500–800 g) were collected from four cage farms every three months from April 2013 to January 2014. Ten antibiotic residues were determined using liquid chromatography-mass spectrometry, and bacteria were isolated and tested for antibiotic resistance to determine the multiple antibiotic resistance (MAR) index. Only three antibiotics (oxytetracycline, tetracycline, and florfenicol) were detected in the muscle of Nile tilapia, and their residues were the highest in small fish; however, the MAR index was higher in large fish. In addition, a direct positive relationship between the MAR index and the concentration of antibiotic residues in Nile tilapia was found. Overall, the adoption of prophylactic management practices improved the sanitary status of cage farms, reducing bacterial infections and hampering the development of antibiotic-resistant bacteria.     

Cow excrements enhance the occurrence of tetracycline resistance genes in soil regardless of their oxytetracycline content

Fertilizing soils with animal excrements from farms with common antibiotic use represents a risk of disseminating antibiotic resistance genes into the environment. In the case of tetracycline antibiotics, it is not clear, however, whether the presence of antibiotic residues further enhances the gene occurrence in manured soils. We established a microcosm experiment in which 3 farm soils that had no recent history of fertilization with animal excrements were amended on a weekly basis (9 times) with excrements from either an oxytetracycline-treated or an untreated cow. Throughout the study, the concentration of oxytetracycline in excrements from the treated cow was above 500 μg g⁻¹ dw, whereas no oxytetracycline was detected in excrements from the healthy cow. Both excrements contained tetracycline resistance (TC-r) genes tet(L), tet(M), tet(V), tet(Z), tet(Q) and tet(W). The excrements from the treated cow also contained the tet(B) gene, and a higher abundance of tet(Z), tet(Q) and tet(W). Three weeks after the last excrement addition, the individual TC-r genes differed in their persistence in soil: tet(Q) and tet(B) were not detectable while tet(L), tet(M), tet(Z) and tet(W) were found in all 3 soils. There were, however, no significant differences in the total number, nor in the abundance, of TC-r genes between soil samples amended with each excrement type. The oxytetracycline-rich and the oxytetracycline-free excrement therefore contributed equally to the increase of tetracycline resistome in soil. Our results indicate that other mechanisms than OTC-selection pressure may be involved in the maintenance of TC-r genes in manured soils.     

方钢管混凝土柱-钢梁平面框架耐火性能研究

建立了局部火灾下多层多跨矩形钢管混凝土柱-钢梁平面框架温度场和力学性能分析的有限元模型。在考虑楼板影响的基础上,研究了保护层厚度不同时钢管混凝土框架结构的温度场分布规律。研究了不同受火工况条件下钢管混凝土框架结构的变形和破坏规律、耐火极限状态、受火梁的内力状态以及结构耐火极限的规律。分析表明,梁保护层厚度影响钢梁温度分布形式;火灾下,框架结构发生了受火梁的整体屈曲破坏。     

Assessment of vancomycin resistance transfer among enterococci of clinical importance in milk matrix

Abstract

Dissemination of vancomycin resistance in enterococci has been associated with horizontal transfer of mobile genetic elements. Aim of the study was to evaluate if milk matrix is a suitable environment to support transferability of vancomycin resistance (vanA) gene from clinical vancomycin-resistant Enterococcus faecium to vancomycin-sensitive Enterococcus faecalis. Enterococci strains were firstly screened for the presence of cpd (inducible sex pheromone determinant) gene, vanA and tetL genes (vancomycin and tetracycline resistance markers, respectively) and the gelE (extracellular metalloendopeptidase) gene to define the mating pairs. Based on these selection markers, we investigated the transferability of eight plasmid-borne vanA harbored by E. faecium (vanA+, cpd-, tetL- and gelE-) into two E. faecalis (vanA-, cpd+, tetL?+?and gelE+) recipient strains in milk matrix. The strains were mated in a 1:1 ratio in 7% reconstituted milk and incubated at 37?°C. Transconjugants emerged from all 16 matings within 2?h of incubation and were evidenced by dual antibiotic resistance (vancomycin and tetracycline). The vancomycin-resistance of trasconjugants was maintained even after ten subsequent passages on nonselective medium. Transconjugants were positive for vanA, tetL and gelE genes. This study indicates milk matrix as suitable environment to support gene exchange between Enterococcus species.