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621.
622.
Plankton community composition in the Three Gorges Reservoir Region
revealed by PCR-DGGE and its relationships
with environmental factors 总被引:1,自引:0,他引:1
To explore the relationships between community composition and the environment in a reservoir ecosystem, plankton communities from the Three Gorges Reservoir Region were studied by PCR-denaturing gradient gel electrophoresis fingerprinting. Bacterial and eukaryotic operational taxonomic units (OTUs), generated by DGGE analysis of the PCR-amplified 16S and 18S rRNA genes, were used as surrogates for the dominant "biodiversity units". OTU composition among the sites was heterogeneous; 46.7% of the total bacterial OTUs (45) and 64.1% of the eukaryotic OTUs (39) were identified in less than half of the sampling sites. Unweighted pair group method with arithmetic averages (UPGMA) clustering of the OTUs suggested that the plankton communities in the Xiangxi Rive sites were not always significantly different from those from the Yangtze River sites, despite clear differences in their environmental characterizations. Canonical correspondence analysis (CCA) was applied to further investigate the relationships between OTU composition and the environmental factors. The first two CCA ordination axes suggested that the bacterial community composition was primarily correlated with the variables of NO3^--N, dissolved oxygen (DO), and SiO3^2--Si, whereas, the eukaryotic community was mainly correlated with the concentrations of DO, PO4^3--P, and SiO3^2--Si. 相似文献
623.
Methamidophos was widely used a pesticide in northern China. The potential influences of methamidophos on soil fungal community in black soil were assessed by plate count, 28S rDNA-PCR-DGGE, and clone library analysis. Three methamidophos levels (50, 150, and 250 mg/kg) were tested in soil microcosms. Results from plate count during a 60-d microcosm experiment showed that high concentrations of methamidophos (250 mg/kg) could significantly stimulate fungal populations. DCGE (denaturing gradient gel electrophoresis) fingerprinting patterns showed a significant difference between the responses of culturable and total fungi communities under the stress of methamidophos. Shannon diversity indices calculated from DGGE profiles indicated that culturable fungi in all microcosms with methamidophos treatment increased after 1 week of incubation. However, the diversity indices of total fungi decreased in the first week, as compared to the stimulation of culturable fungi. At the 8th week, however, all the microcosms treated by methamidophos were similar to the control microcosms in community structure as suggested by the Shannon diversity indices for both culturable and total fungi. In contrast, after 1 week the fungal structure of culturable and unculturable both were disturbed to different extent under the stresses of methamidophos by clustering analysis. Clone sequencing analysis indicated the stimulation of pathogenic and unculturable fungal populations by methamidophos treatment, suggetsing potential risks of plant disease outbreak. 相似文献
624.
为提升应急救援人员防护服抗爆能力,减少爆炸事故中人员伤亡数量,以内凹蜂窝型梯度结构为研究对象,采用爆炸冲击实验与数值有限元法相结合的研究手段,通过改变胞元尺寸与胞元凹角对梯度蜂窝结构进行优化,分析梯度结构在爆炸冲击环境下冲击波衰减效率和力学响应规律。结果表明:同等质量条件下,内凹蜂窝结构尺寸梯度为2.58 mm-3.40 mm-2.58 mm、角度梯度30°-22.5°-30°时对冲击波的衰减效应最佳,衰减效率分别为85.44%,82.29%。研究结果可为同时满足轻质、高强抗爆要求的防护服结构设计提供技术支撑。 相似文献
625.
626.
反硝化除磷与厌氧氨氧化耦合可进一步降低脱氮除磷所需的碳源,而稳定获取NO2--N是两工艺独立、联合运行的关键.因此,以低C/N生活污水为研究对象,接种絮状污泥及少量长期贮存的好氧颗粒污泥,采用两段梯度曝气,并控制停曝时间(3min:3min),即以高频间歇梯度曝气模式,经60d的富集培养,将其诱导成具有短程硝化反硝化除磷功能的颗粒污泥,并分析了此过程中系统脱氮除磷性能变化.结果表明,稳定时期内,颗粒污泥平均粒径达841μm,SVI为31.23ml/g,颗粒结构致密,沉降性能良好.出水NO3--N小于0.1mg/L,TP<0.7mg/L,NO2--N>15mg/L,实现了P的高效去除和NO3--N的积累,并可为后续耦合Anammox提供稳定NO2--N基质.批次实验结果表明,颗粒中可利用NO2--N为电子受体的DPAOs占达57.63%,其富集提高了系统除磷能力.高频梯度间歇曝气可实现AOB与DPAOs的高度耦合,但仅以间歇曝气难以实现NOB的抑制,后通过在"氨谷点"前段,加入梯度曝气优化实际限氧曝气点,可强化对NOB的抑制,从而实现良好的亚硝酸盐积累. 相似文献
627.
不同来流下火旋风的实验研究 总被引:1,自引:0,他引:1
已有的实验研究和数值模拟表明:火旋风存在一种中空燃烧的状态.本文通过燃烧风洞,对不同来流影响下的火旋风进行分析研究.通过一个置于大型燃烧风洞中的边角开有切向进风口的六边形小尺寸火旋风发生模型,应用热电偶测温和皮托管测速以及电子数据采集系统,对该模型内的温度分布数据和速度分布数据进行采集.通过燃烧风洞改变来流速度的大小,分析不同来流速度下火旋风内部的温度分布、速度分布和持续时间,研究来流速度对火旋风内部燃烧结构的影响、火旋风旋转速度的影响以及持续时间的影响.研究表明:来流改变了火旋风内部的燃烧结构,促进了中空燃烧状态的形成.中空燃烧状态有一个中心的低温区域.在中心低温区域温度最低时,火旋风持续时间最长.来流增强了火旋风的整体转动,并且加剧了火旋风的螺旋状上升. 相似文献