白腐真菌生物膜反应器处理染料生产废水实验研究

研究了使用3种白腐真菌生物膜反应器处理染料生产废水．探讨了脱色．pH．COD去除与运行方式．反应器型式等因素之间的关系。结果表明．⑴间歇式运行中．3种反应器对染料生产废水的主要脱色作用都发生在24h内．脱色速度．最终脱色率和抗杂菌污染的能力均以组合填料生物接触氧化反应器最强．COD去除率却以生物转盘反应器最高。(2)连接式运行中．白腐真菌组合填料生物接触氧化反应器对染料废水的脱色效果．pH变化均与间歇式运行十分相似．最高脱色率达到99％左右．出水pH平均为3．6．但COD的去除率不高．且波动较大。将光合细菌以及活性污泥生物接触氧化反应器串接到白腐真菌生物膜反应器后显著提高了COD去除率．但出水色度并没有继续降低。     

Effect of nitrogen concentration in culture mediums on growth and enzyme production of Phanerochaete chrysosporium

Effect of different nitrogen concentration in the mediums on growth and enzyme production of Phanerochaete chrysosporium was studied when glucose concentration was l0 g/L. The results showed that the medium contained 0.8 g/L ammonium tartrate is the best. It not only supply abundant nutrients for the growth of Phanerochaete chrysospodum, which make mycelia the best grow compared with the other medium, but also produce higher manganese-dependent peroxidase(Mnp) and laccase(Lac) activity. In addition, it is observed that the variation of mycelia surface is related to ligninolytic enzyme secreted by Phanerochaete chrysosporium. When the surface of mycelium pellets appeared burs, it predicts secondary metabolism begin. This experimentation demonstrated that when the ratio of carbon and nitrogen in nitrogen limited medium is equal to 100:8, growth and enzyme production of Phanerochaete chrysosporium is the best, it could achieve the maximum Mnp and Lac activity.     

白腐真菌对染料的脱色与降解

利用白腐真菌对染料脱色和降解的过程机理及影响因素进行了较为系统的介绍,结果认为,白腐真菌对染料的脱色及降解作用可能主要是由于其在次生代谢阶段产生的木质素过氧化酶LIPs和锰过氧化酶MnPs所致。在培养基中加入黎芦醇等能够显著提高LIPs的产生;富氮培养基会抑制LIPs的生成;缓冲液的选择对维持适宜的pH值和菌丝的形态有一定作用,从而影响其脱色效果;富氧环境是一切白腐真菌对染料进行脱色和降解的必要条件;适度的搅拌混合有利于反应时的物质之间传递;一般地,在培养时间达到3d以后白腐真菌才能达到较高的脱色与降解活性。染料分子大小和结构及其基团的位置对脱色及降解效果有明显影响。使用特殊填料来提高处理系统中的生物量以克服真菌生长速度相对较慢,提高处理能力是该技术今后的研究重点。     

真菌降解挥发性有机污染物的特性与影响因素

介绍了利用真菌降解挥发性有机污染物的生物反应器的特性及发展状况。阐述了真菌反应器内填料的选择以及温度、湿度、pH、含氧量等条件对真菌活性的影响     

土壤重金属污染对丛枝菌根真菌产孢量的影响

以沈阳附近重金属矿区自然和农田土壤为研究对象，对土壤重金属含量、植物类型、AMF种群及孢子密度进行了调查，并对污染的农田、荒地土壤进行了比较．结果表明：调查污染区土壤中球囊霉属为优势类群，无梗囊霉属为少见类群，盾巨孢囊霉属为偶见类群，无梗囊霉属和盾巨孢囊霉属对重金属污染特别是铜污染较为敏感；丛枝菌根真菌重金属污染土壤中分布广泛．在综合污染指数达到28．03的严重污染土壤中仍能生存，其孢子密度在不同污染程度的土壤中变异很大，轻、中度污染土壤中较高，随重金属污染程度的增加而锐减；矿区重金属污染土壤中土壤污染程度、土壤pH值、土壤利用情况及宿主植物都影响AMF产孢．图4表4参32     

Growth promotion and disease prevention effects of streptomyces on Amorphophallus konjac K. Koch ex N.E.Br北大核心CSCD

This study aimed to evaluate the disease antagonism and seed growth promotion effects of streptomyces on Amorphophallus konjac by agar block and axenic fermentation extract experiments. Pot experiments were taken to assess relative biocontrol effiency and effect of streptomyce preparation M (mixture of S. carnosus and S. pactum in solid-state fermentation with 1:1 ratio) on biomass, photosynthetic rate, yield and corm quality of A. konjac K. Koch ex N.E.Br. Results showed that: 1 Streptomyces Act12 had obvious antagonistic activity against six soft rot pathogens, and the diameter of growth inhibition zones ranged from 19.4 to 31.6 mm. Fermentation filtrate of streptomyces exhibited growth-promoting activity on melon seed germination and seedling growth. 2 Streptomyce-derived viable preparations had biocontrol effect on A. konjac regarding soft rot-disease prevention. In the pot experiment, individual inoculation of preparations achieved 65.0% relative biocontrol effect on A. konjac during the harvest stage. After 10 and 20 days of inoculation combined with Pectobacterium chrysanthemi (a bacterial pathogen), the relative biocontrol effiency of streptomyces were 100.0% and 58.4%, respectively. 3 Streptomyces showed obvious growth-promoting effect on growth and yield of A. konjac. In the pot experiment, combined inoculation of streptomyces and Pectobacterium chrysanthemi significantly increased the petiole diameter by 11.1%, net photosynthetic rate by 111.7%, fresh weight of corm by 54.8%, fresh weight of rhizome by 304.2%, and the number of rhizomes by 200.0%, compared with individual inoculation of Pectobacterium chrysanthemi. 4 Individual inoculation with streptomyces improved corm quality of A. konjac. The contents of glucomannan, starch and cellulose significantly increased by 14.0%, 8.1% and 27.7%, respectively compared with control (P < 0.05) . This study indicates that streptomyces promotes the growth of A. konjac, enhances the yield and the content of glucomannan, and at the same time helps disease prevention of A. konjac.     

Cadaver decomposition in terrestrial ecosystems

A dead mammal (i.e. cadaver) is a high quality resource (narrow carbon:nitrogen ratio, high water content) that releases an intense, localised pulse of carbon and nutrients into the soil upon decomposition. Despite the fact that as much as 5,000 kg of cadaver can be introduced to a square kilometre of terrestrial ecosystem each year, cadaver decomposition remains a neglected microsere. Here we review the processes associated with the introduction of cadaver-derived carbon and nutrients into soil from forensic and ecological settings to show that cadaver decomposition can have a greater, albeit localised, effect on belowground ecology than plant and faecal resources. Cadaveric materials are rapidly introduced to belowground floral and faunal communities, which results in the formation of a highly concentrated island of fertility, or cadaver decomposition island (CDI). CDIs are associated with increased soil microbial biomass, microbial activity (C mineralisation) and nematode abundance. Each CDI is an ephemeral natural disturbance that, in addition to releasing energy and nutrients to the wider ecosystem, acts as a hub by receiving these materials in the form of dead insects, exuvia and puparia, faecal matter (from scavengers, grazers and predators) and feathers (from avian scavengers and predators). As such, CDIs contribute to landscape heterogeneity. Furthermore, CDIs are a specialised habitat for a number of flies, beetles and pioneer vegetation, which enhances biodiversity in terrestrial ecosystems.     

无公害食用菌生产技术规程的制定研究

通过分析近年来我国食用菌生产管理和产品质量安全存在的问题,论述了开展无公害食用菌生产的重要性.同时吸收各地的生产经验.根据国家有关标准,从食用菌生产场所的选择、培养基材料和水的要求、栽培管理、病虫害防治、采后处理、加工贮藏和产品质量安全检测等几个方面,提出了一整套较系统的无公害食用菌生产技术操作规程.该项技术规程在生产上的推广应用,对提高食用菌生产技术水平、改善和保障食用菌产品质量、增强产品在国际市场的竞争力将产生积极的推动作用.     

Antifungal efficacy of some natural phenolic compounds against significant pathogenic and toxinogenic filamentous fungi

In terms of food safety, species of the Fusarium, Aspergillus and Penicillium genera are considered the most significant because they produce the great majority of known mycotoxins. Developing resistance against commonly used fungicides have become a critical problem in area such as agriculture, the storage and production of food and even in human medicines. The need for research and development of new alternative antifungal treatment based on natural antifungal substances is obvious. Here, the antifungal efficacy of 21 phenolic components of essential oils and plant substances were tested against these filamentous fungi with respect to their different molecular structures. Minimum inhibitory concentration values MIC₅₀ and MIC₁₀₀ were successfully estimated for 15 substances by means of probit analysis. Thymol and carvacrol were evaluated as the most effective. The MIC₅₀ values for thymol ranged from 30 to 52 μg mL⁻¹. The MIC₁₀₀ values for thymol ranged from 76 to 255 μg mL⁻¹, respectively. For carvacrol, the MIC₅₀ values ranged from 37 to 76 μg mL⁻¹, and the MIC₁₀₀ ranged from 131 to 262 μg mL⁻¹. The results also revealed differences in the efficacy of phenols depending on molecular structures and different inter-species sensitivity.     

白腐真菌的广谱生物降解性研究进展

白腐真菌由于能够降解木质素而在地球的碳循环中发挥着不可或缺的作用.由胞外的过氧化物酶类和其他次级代谢产物组成的木质素降解系统除了能够降解木质素外,对众多的异生物质也具有广谱的生物降解性,赋予了白腐真菌巨大的环境工业应用潜力.对白腐真菌的木质素降解系统和其广谱的生物降解性进行了介绍与展望.