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11.
Dr Marianne Johansen Marian Knight Edward J. Maher Kim Smith Ian L. Sargent 《黑龙江环境通报》1995,15(10):921-931
Trophoblast deportation is known to occur in normal human pregnancy, but it is not yet clear whether these cells routinely enter the maternal peripheral circulation and are available as a source of fetal DNA for non-invasive prenatal diagnosis of genetic disorders. To resolve this issue requires an efficient method of enriching trophoblast from maternal blood combined with a means to confirm its identity. Five different techniques were tested on ten retroplacental blood samples to determine the most sensitive and operator-efficient method. Lysis of red cells alone gave the best recovery of trophoblast but had to be discounted, together with Ficoll density gradient centrifugation, due to the very low purity and the excessive time required. Fluorescence-activated cell sorting (FACS) of pre-enriched trophoblast resulted in the lowest recovery rate (8 per cent) despite a 3250-fold enrichment and a very high purity. Immunomagnetic beads (Dynabeads) coated with anti-CD 16 antibody proved to be the best method for the subsequent immunocytochemical characterization of deported trophoblast. However, IO beads coated with anti-CD45 antibody may be more useful for isolating trophoblast for prenatal diagnosis due to the high purity, enrichment (32-fold), and recovery rate (78 per cent) obtained with this method. 相似文献
12.
At a rice-wheat rotational free-air CO2 enrichment(FACE) platform, the effect of elevated atmospheric CO2 on soil nematode communities in a farmland ecosystem was studied. Wheat plots were exposed to elevated atmospheric CO2 (ambient 370 μl/L 200 μl/L).32 families and 40 genera of nematode were observed in soil suspensions during the study period. Under FACE treatment, the numbers of total nematodes, bacterivores and fungivores exhibited an increasing trend. Because of the seasonal variation of soil temperature and moisture, the effect of elevated atmospheric CO2 on soil nematodes was only observed under favorable conditions. The response of nematode communities to elevated atmospheric CO2 may indicate the change of soil food web. 相似文献
13.
Dr. Lauren Lynch James Bussel James D. Goldberg Usha Chitkara Isabelle Wilkins Jan Macfarland Richard L. Berkowitz 《黑龙江环境通报》1988,8(5):329-331
A case is presented in which percutaneous umbilical sampling (PUBS) was utilized in the second and third trimesters for the diagnosis and management of a pregnancy at risk for neonatal alloimmune thrombocytopenia (NAIT). 相似文献
14.
Yuen Tannirandorn Umberto Nicolini Peter C. Nicolaidis Nicholas M. Fisk Sabaratnam Arulkumaran Charles H. Rodeck 《黑龙江环境通报》1990,10(3):189-193
Twelve second-trimester fetuses with cystic hygroma underwent fetal blood sampling for rapid karyotyping, haematologic evaluation, and blood gas analysis. An abnormal karyotype was found in seven cases: monosomy X in five, trisomy 21 in one, and trisomy 13 in the other. Eight often fetuses undergoing blood gas analysis showed hypoxaemia, five of which were growth-retarded. Nine pregnancies were terminated. Of the remaining three, only one fetus survived the perinatal period. 相似文献
15.
本试验在10±1℃、不喂食条件下测定鲤鱼在30天受汞毒期间鳃、脑和肝胰脏蓄积汞和尾鳍微血管血流速度及30天解毒期间排出汞和血流速度的变化。试验结果:受毒期间鳃中汞的蓄积量最多,解毒期间鳃中汞的排出速度最快;受毒期间血流速度随受毒时间的延长而减慢,又随试验浓度的增高而减慢,解毒期间血流速度随解毒时间的延长而加快;受毒期间高浓度试验组鱼鳃、脑和肝胰脏中汞的蓄积量与血流速度呈负相关,解毒期间鱼鳃中汞的排出量与血流速度呈正相关,汞的浓度与血流速度呈负相关。 相似文献
16.
We report on a case of trisomy 8 mosaicism detected prenatally in a single clone of amniotic fluid culture, and confirmed on fetal blood and on peripheral lymphocytes after birth. A follow-up was performed over 3 years, showing a clinically normal female with cognitive, neuropsychological, and linguistic development in a normal range. 相似文献
17.
We report the first prenatal diagnosis of an affected fetus with Chediak-Higashi syndrome (CHS). Diagnosis was accomplished via fetal blood sampling at 17 menstrual weeks and was confirmed after birth. Retrospective measurement of the largest acid phosphatase-positive lysosomes in cultured amniotic fluid cells and chorionic villus cells showed that in CHS these lysosomes are significantly larger than those in normal cells. This method may be used for prenatal diagnosis of CHS by amniocentesis and chorionic villus sampling (CVS). 相似文献
18.
在对大背坞地区双桥山群变质岩中“斑点构造”研究的基础上发现了同生碎屑-一种发育于沉积岩中反映动荡沉积环境的典型沉积构造,为进一步证实双桥山群属于半深海-深海的浊流相沉积环境特点提供重要依据,通过详细的构造变形分析,确认双桥山群主要经历了区域紧闭褶皱和韧性剪切两期构造变形,运用平衡剖面方法和应变测量估算双桥山群褶皱变形的地壳缩短量为71.6%,双桥山群地层层内压扁变形压缩率为23.1%~45.3%,在对大背钨韧性剪切带进行系统研究后,建立了一套浅变质碎屑中进变质韧性剪切带的识别标志,进而对该区韧性剪切带进行 划分和厘定,同时依据韧性剪切带中同生碎屑和同构造变斑晶的应变测量结果(Kxz值),估算出大背坞韧性剪切带剪应变(γ)为1.01~2.13、最大剪位移量为0.6~0.8km。结合区域构造演化分析,认为双桥山群褶皱变形形成于晋宁期,韧性剪切变形发生于燕山期,前者是扬子地块基底岩系形成时期,后者是鹅湖花岗岩侵位和隆升过程中引发的花岗岩外围地区变质岩的以垂向剪切运动为特征的韧性剪切变形作用。 相似文献
19.
《环境科学学报(英文版)》2023,35(4):517-530
Graphite carbon nitride has many excellent properties as a two-dimensional semiconductor material so that it has a wide application prospect in the field of photocatalysis. However, the traditional problems such as high recombination rate of photogenerated carriers limit its application. In this work, we introduce nitrogen deficiency into g-C3N4 to solve this problem a simple and safe in-situ reduction method. g-C3N4/CaCO3 was obtained by a simple and safe one-step calcination method with industrial-grade micron particles CaCO3. Cyano group modification was in-situ reduced during the thermal polymerization process, which would change the internal electronic structure of g-C3N4. The successful combination of g-C3N4 and CaCO3 and the introduction of cyanide have been proved by Fourier transform infrared spectroscopy and X-ray photoelectron spectrometer. The formation of the cyano group, an electron-absorbing group, promotes the effective separation of photogenic electron hole pairs and inhibits the recombination of photogenic carriers. These advantages result in the generation of more •O2− and 1O2 in the catalytic system, which increases the photocatalytic efficiency of nicotine degradation by ten times. Furthermore, the degradation process of nicotine has been studied in this work to provide a basis for the degradation of nicotine organic pollutants in the air. 相似文献
20.