全文获取类型
收费全文 | 562篇 |
免费 | 35篇 |
国内免费 | 89篇 |
专业分类
安全科学 | 98篇 |
废物处理 | 9篇 |
环保管理 | 43篇 |
综合类 | 303篇 |
基础理论 | 128篇 |
污染及防治 | 45篇 |
评价与监测 | 17篇 |
社会与环境 | 23篇 |
灾害及防治 | 20篇 |
出版年
2024年 | 1篇 |
2023年 | 9篇 |
2022年 | 15篇 |
2021年 | 11篇 |
2020年 | 22篇 |
2019年 | 16篇 |
2018年 | 24篇 |
2017年 | 15篇 |
2016年 | 21篇 |
2015年 | 20篇 |
2014年 | 11篇 |
2013年 | 73篇 |
2012年 | 32篇 |
2011年 | 55篇 |
2010年 | 20篇 |
2009年 | 33篇 |
2008年 | 17篇 |
2007年 | 37篇 |
2006年 | 19篇 |
2005年 | 31篇 |
2004年 | 15篇 |
2003年 | 18篇 |
2002年 | 15篇 |
2001年 | 17篇 |
2000年 | 12篇 |
1999年 | 8篇 |
1998年 | 14篇 |
1997年 | 4篇 |
1996年 | 14篇 |
1995年 | 29篇 |
1994年 | 8篇 |
1993年 | 6篇 |
1992年 | 5篇 |
1991年 | 4篇 |
1990年 | 6篇 |
1989年 | 3篇 |
1988年 | 6篇 |
1987年 | 5篇 |
1986年 | 2篇 |
1984年 | 3篇 |
1983年 | 6篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1980年 | 1篇 |
排序方式: 共有686条查询结果,搜索用时 31 毫秒
141.
Antonio Cao Angela M. Falchi Teresa Tuveri Maria T. Scalas Giovanni Monni Cristina Rosatelli 《黑龙江环境通报》1986,6(3):159-167
In this report we have summarized our experience with the prenatal diagnosis of β-thalassemia in 1000 pregnancies followed at least until 12 months after birth. In the majority of these cases, the thalassemia lesion was the nonsense mutation at the codon corresponding to amino acid 39, which produces the hematological phenotype of β-thalassemia. Fetal blood sampling was carried out by placental aspiration, by which a sufficient amount of fetal blood for analysis was obtained in the majority of cases (99 per cent). The fetal mortality associated with fetal blood sampling was 6·3 per cent. Those placental samples contaminated by maternal cells were successfully purified by Ørskov lysis. Fetal blood was analysed by globin chain synthesis on CM–52 columns, which gave reliable results. Two misdiagnoses (0·2 per cent) have been made of which one was due to a non-globin protein co-migrating with the β-chains while the other resulted from a misclassification of the type of thalassemia segregating in the family. 相似文献
142.
143.
This paper compares the results of a survey of plasma creatine kinase (CK) activity measured in fetuses at-risk for Duchenne muscular dystrophy (DMD) with a reliable control series. Only pure fetal blood samples obtained by fetoscopy at between 17–24 weeks gestational age were used. Of the at-risk group 19 male pregnancies, mostly at low risk for DMD, proceeded to term with a normal outcome; there was no significant difference between their fetal plasma CK activities and the control group. Another 21 male pregnancies were terminated. This group included the highest risk mothers and hence was expected to contain a significant proportion of affected fetuses. The fetal plasma CK activity range was overlapping but significantly higher than the control group. No grossly elevated CK value was obtained. We conclude that, on average, DMD fetuses at this gestational age have higher plasma CK activity than controls. The problems of applying this finding to the prenatal diagnosis of DMD are discussed. 相似文献
144.
西藏措勤地区年波组岩石地球化学特征 总被引:1,自引:0,他引:1
林子宗群年波组为始新世火山岩地层,岩性主要为一套中-酸性火山熔岩、火山碎屑岩,局部夹淡水灰岩.野外地质调查和室内岩石学、岩石地球化学等综合研究成果显示这些岩石属钙碱性系列岩石,不相容元素K、Rb、Ba、Th强烈富集和Nb、Sr、P、Ti等元素明显亏损等特征,且明显受区域断裂构造控制,与区域构造演化有密切的内在联系.研究这一地史时期的火山岩的分布、产状、岩石学、岩石地球化学和同位素年代学,对于进一步认识青藏高原的形成和隆升不仅具有重要的科学意义,而且将为探寻与火山活动有关的矿产资源提供基础地质资料. 相似文献
145.
DM Peter Lorenz Rainer Bollmann Georg Klaus Hinkel Marco Mächler Gabriele Siegert Gudrun Stamminger Jörg Wendisch Sabine Ziemer 《黑龙江环境通报》1991,11(11):819-825
The study of the fetal platelet count and size can, according to the literature, be used for the prenatal diagnosis of the Wiskott-Aldrich syndrome (WAS). So far, no affected fetuses have been identified by this method. All pregnancies in which this method had been applied to resulted, as correctly predicted, in the birth of normal children. Here we report on a familial case of WAS where the haematological parameters failed to reveal the affected second child. Hence we assume that the platelet count and size of platelets remain normal in fetuses with WAS to the gestational age of 22 weeks and cannot be used for prenatal diagnosis. 相似文献
146.
Kathryn Andrews Johannes Wienberg Malcolm A. Ferguson-Smith David C. Rubinsztein 《黑龙江环境通报》1995,15(10):913-919
The presence of small numbers of fetal nucleated red cells in the maternal circulation has been a stimulus for the development of technologies for non-invasive prenatal genetic analysis. Our laboratory has been assessing the feasibility of density gradient centrifugation followed by magnetic activated cell sorting (MACS) of cells expressing CD32 and CD45, to deplete maternal nucleated blood cells. We have examined the efficiency of each of the steps of this procedure using cord blood from term pregnancies as a source of nucleated red blood cells. Cord blood was shown to contain highly variable numbers of nucleated red cells. Three different density gradients were examined. There was no major difference in the performances of the double and triple gradients. Density gradient centrifugation resulted in enrichments of nucleated red blood cells of about 1000-fold relative to the total cell count. However, it was apparent that the selection of the cell layers which were most enriched for these cells would result in significant losses of nucleated red cells in other layers. MACS sorting of cells using CD45 resulted in white cell depletions ranging from 7 to 34-fold. These data provide a foundation for comparison with other methods and for optimization of the MACS technique. 相似文献
147.
W. Lissens M. Van Lierde J. Decaluwe W. Foulon P. Evrard F. van Hoof M. Freund I. Liebaers 《黑龙江环境通报》1988,8(1):59-62
The X-linked Hunter syndrome or mucopolysaccharidosis II was diagnosed in a male fetus by demonstrating a severe deficiency of iduronate 2-sulphate sulphatase activity in fetal plasma obtained by umbilical fetal blood sampling at 23 weeks of pregnancy. The diagnosis was confirmed after termination of pregnancy. 相似文献
148.
两性高分子絮凝剂阴、阳离子基团含量对絮凝性能的影响 总被引:9,自引:0,他引:9
合成了一系列带不同阴、阳离子基团含量的高分子絮凝剂,详细讨论了阴、阳离子基团含量对硅藻土悬浮体系絮凝性能(透光率、沉降速度、沉降体积)的影响。结果表明:阴、阳离子基团含量并不是越大越好,它们之间有个协同效应,基团含量太大或太小对絮凝作用都不利。 相似文献
149.
M. R. Thomas B. Tutschek A. Frost C. H. Rodeck N. Yazdani I. Craft R. Williamson 《黑龙江环境通报》1995,15(7):641-646
A single copy Y-chromosome DNA sequence was amplified using the polymerase chain reaction (PCR) from the peripheral blood of 30 women who had achieved a pregnancy through an in vitro fertilization (IVF) programme. The time of conception was known precisely and was confirmed by serial ultrasound scans. Conceptions were dated as the number of weeks after fertilization plus 2, to give a time equivalent to the obstetric menstrual dating of the pregnancy (LMP). Y-chromosome-specific DNA was detected in all pregnancies with a male fetus (18/30). The earliest detection was at 4 weeks and 5 days, and the latest at 7 weeks and 1 day. Y-chromosome-specific sequences were no longer detected in any of the male pregnancies 8 weeks after delivery. No Y-chromosome sequences were detected in any of the pregnancies where only female babies were delivered. This demonstrates that fetal DNA appears in the maternal circulation early in the first trimester, that it can be identified in all pregnancies tested by 7 weeks, that it continues to be present throughout pregnancy, and that it has been cleared from the maternal circulation 2 months after parturition. Early non-invasive prenatal diagnosis for aneuploidies and inherited disorders will be possible in all pregnancies if fetal cells can be isolated free from maternal contamination (or identified accurately in the presence of maternal cells) without problems of contamination from previous pregnancies. 相似文献
150.
F. Daffos F. Forestier J. Mac Aleese C. Aufrant L. Mandelbrot E. A. Cabanis M. T. Iba-Zizen J. M. Alfonso J. Tamraz 《黑龙江环境通报》1988,8(4):311-314
Fetal magnetic resonance (MR) imaging was performed at 33 weeks of gestation for investigation of a posterior fossa abnormality found at ultrasound screening. Fetal movements were abolished by vecuronium injected under ultrasound guidance into the umbilical vein. MR images showed atrophy of the left cerebellar lobe with cisternal dilatation. These were confirmed postnatally by CT scan. 相似文献