A_2型高粱细胞质雄性不育系与其保持系的胞质DNA和核DNA差异

以高粱细胞质雄性不育系A2 V4 (A)及其保持系V4 (B)的总DNA为模板 ,对 184个随机引物进行筛选 ,找到6个其RAPD扩增产物在A/B间存在稳定差异的引物 .将该 6个引物同时扩增A/B的总DNA、线粒体DNA(mtDNA)及叶绿体DNA(cpDNA) .以总DNA为模板时得到 12个扩增片段 ,以mtDNA为模板时得到 4个 ,以cpDNA为模板时得到 11个 .结果分析表明 ,在这些扩增片段中 ,有 7个仅仅出现在以胞质DNA为模板的扩增中 ,有 5个在以总DNA和胞质DNA为模板时同时出现 ,即认为这 12个片段来自胞质DNA .另有 7个片段 ,在以胞质DNA为模板时未出现 ,而是仅仅出现在以总DNA为模板的扩增中 ,认为是来自核DNA .来自核DNA的 7个扩增片段中 ,有 5个来自保持系 ,有 2个来自不育系 ,这表明 ,不育系与保持系在核DNA上存在差异 .对A/B核DNA在CMS中的重要性及研究对策进行了讨论 .图 2表 4参 19     

区域污染治理能力与排污规模的协调性评价

为分析区域排污规模与污染治理的协调性,构建了排污规模与污染治理协调度模型,并以2006、2013年的数据对模型进行实证分析。结果表明,制约排污规模和污染治理协调性的主要矛盾是污染治理能力的落后,全国70%以上地区污染治理能力均滞后于污染物产生规模;国内治理能力在研究期间不断提高,区域差异在不断缩小,治理能力过度滞后的省份由14个减少到2个;除江苏外2013年其余各地区均有1项或多项污染物的治理能力滞后,其中生活废水治理能力滞后的区域最多;经济发展水平是影响协调性的重要因素,2013年协调度与地区人均GDP基本呈线性关系。模型实证计算结果与主观判断也较为吻合,具有一定的推广应用价值。     

基于区位商的我国流域水质污染空间差异及影响因素研究

我国十大流域河流污染的空间分布集聚性较为显著,运用区域经济学中的区位商理论和方法来分析十大流域水污染状况的差异性,结果表明,海河、淮河、黄河和辽河的污径比在2002—2012年均大于0.04,2012年污径比的区位商均达2.6以上,并在11年间整体呈升高态势,表明全国河流污染在这4个流域呈现集聚现象,十大流域水质污染的空间差异性不断加深。从水资源量、污染排放、经济社会发展的角度分析海河、淮河、黄河和辽河污染较重的原因,其中单位水资源污染物负荷较重是直接原因,水资源短缺、经济发展和人口集聚导致水资源承载力不堪重负,而生活污染愈加严重、重污染行业集聚效应以及面源污染加剧都给水质改善带来巨大压力,亟需在这些流域加大综合减排力度。     

江苏省种粮补贴区域差异化理论与实证研究

粮食种植补贴是农业补贴的核心内容。从现行种粮补贴政策出发,构建了差异化情景下种粮补贴效率的理论命题,并认为差异化补贴更体现 “效率优先,兼顾公平”原则;在此基础上,构建了种粮补贴区域差异化指标体系及计算方法并对江苏省进行实证研究。结果发现：江苏省种粮补贴应呈现苏北>苏中>苏南的态势,最高值为总额的182%,分布在苏北地区,最低值为总额的117%,分布在苏南地区;各评价指标空间分布差异显著,补贴政策地区间指向应有所不同;最后提出差异控制系数θ,通过对θ的选择,可以较为有效的控制种粮补贴差异波动范围,为政策的制定提供简便的手段     

Determination of 28 pesticides applied on two tomato cultivars with a different surface/weight ratio of the berries,using a multiresidue GC-MS/MS method

The behavior of 28 pesticides on two tomato cultivars with a different surface/weight (S/W) ratio of the berries (S/W, Birikino vs. Tombola) was studied, in order to provide appropriate indications about their persistence on crops reaching the pre-harvest interval (PHI). Quantitative analysis was performed using a GC MS/MS method. Birikino cv. (BIR) was a “cherry type tomato” with a double S/W ratio compared with Tombola cv. (TOM). The results showed a different pesticide behavior. Azoxystrobin, Boscalid, Bupirimate, Difenoconazole, Etofenprox, Iprodione, Mepanipyrim, Myclobutanil, Tebuconazole, Zoxamide, Metalaxyl M, Pyrimethanil, Tetraconazole, Benalaxyl, Cyprodinil, Fenamidone, Famoxadone and Fludioxonil immediately after treatments showed residues on BIR higher than TOM, and this behavior is consistent with its greater exposed surface. BIR showed higher decay rates of these pesticides during the whole trial, nevertheless residue averages remained higher than TOM reaching the time of harvest. Residues at the PHI were all below their Maximum Residue Levels (MRLs), but data indicated that they could exceed their legal limits especially if the above-mentioned active ingredients were employed more than once per crop cycle on cherry type tomatoes. As regards to Chlorpyrifos, Chlorpyrifos methyl, Triadimenol, Pyridaben and Tebufenpyrad, no different residual behavior related to S/W ratio of the cultivars was observed. Even in this case, residues at the PHI were all below MRLs. As for Cyfluthrin, Deltamethrin, Lambda cyhalothrin, Etoxazole and Cyproconazole, residues were lower than the limit of quantitation (LOQ) of the analytical method just after the treatment, according to their low doses of employment.     

Radon diffusion in an anhydrous andesitic melt: a finite difference solution

Radon-222 diffusion in an anhydrous andesitic melt was investigated. The melts were glass discs formed artificially from melted volcanic materials. Solutions of the relevant diffusion equations were done by the explicit finite difference method. Results were compared to analytical solutions reported in the literature and good agreement was found. We have shown that the explicit finite difference method is effective and accurate for solving equations that describe ²²²Rn diffusion in andesitic melts, which is especially important when arbitrary initial and boundary conditions are required.     

Gender differences in driver's license theory test scores in the Netherlands

INTRODUCTION: Gender differences were investigated in a sample of persons (N=34,755) who completed the driver's license theory test in the Netherlands. RESULTS: Contrary to recent findings from Sweden, no gender differences were found. IMPACT OF INDUSTRY: The present study signifies the importance of standardization in driver testing.     

Differences in urban and rural accident characteristics and medical service utilization for traffic fatalities in less-motorized societies

Introduction

Fatalities from traffic accidents in less-motorized societies are an important global issue. We aimed to characterize the geographic differences of fatalities in such societies to facilitate the development of targeted interventions.

Method

This study linked police reports, hospital data, and vital registration data from Taiwan with special reference to accident factors in pre-hospital deaths and medical care in hospital deaths.

Results

A higher percentage of pre-hospital deaths were observed following rural as compared to urban traffic accidents. The deaths due to rural accidents can be attributed to lower use of restraints (i.e., helmets or seat belts), lower percentage of motorcyclists, and more highway accidents. A higher percentage of victims in rural accidents were transported to distant medical centers rather than to local hospitals.

Conclusion

Specific interventions, such as intelligent emergency medical systems, campaigns for helmets and seat belt usage, enforcement of helmets and seat belt use, and speed control measures should be targeted to rural areas.

Impact on industry

Cooperation between the vehicle industry and emergency medical providers in rural traffic accident rescue teams may decrease the numbers of deaths in these regions.     

海啸波近岸共振响应的数值模拟及分析

应用有限差分方法求解非线性浅水长波方程,建立了海啸波产生和传播的二维数值模型;对太平洋地震引起的夏威夷群岛海啸波进行模拟,并将模拟结果和测站实测值进行比较,验证了模型的正确性。利用快速傅里叶变换对数值模拟结果进行谱分析,得到整个计算区域的能量谱分布,并给出了发生能量聚集的位置及相应的谱峰周期。讨论了海啸波传播到近岸时可能产生的共振响应现象,发现海啸波和近岸的共振响应不仅与近岸复杂地形有关,还与海啸波传播到近岸时的波浪入射方向有关。     

气密性高等级生物安全实验室的负压控制

提出漏风量换气次数的概念,并以此作为评价气流控制阀是否适用于生物安全实验室压差控制的指标;提出引入附加漏风量的气密性生物安全实验室压差控制策略。进行对比实验,研究实验室在无人为漏风量与引入人为漏风量这两种情况下启动、微扰动、关门等工况下实验室的压差波动情况,其结果显示在引入人为漏风量情况下实验间压差波动范围减小、稳定速度加快。从而说明引入附加漏风量的压差控制策略在气密性生物安全实验室的压差控制方面具有重要的实际应用价值。