用好防灾科技学院教育平台创建和弘扬防震减灾文化-恭贺母校升本暨校庆30周年

本文对防震减灾文化的组成、创建动力及其方法进行了探讨.初次提出了防震减灾文化的概念,并对创建防震减灾文化的目的和如何创建、弘扬防震减灾文化提出了看法和观点.     

用好防灾科技学院教育平台 创建和弘扬防震减灾文化——恭贺母校升本暨校庆30周年

本文对防震减灾文化的组成、创建动力及其方法进行了探讨。初次提出了防震减灾文化的概念，并对创建防震减灾文化的目的和如何创建、弘扬防震减灾文化提出了看法和观点。     

可持续发展概念指导下的化学--绿色化学

阐述了人类文明发展到绿色文明阶段时所产生的化学思想方法与传统化学思想方法的差别,分析了作为绿色文明积极实践者的绿色化学的产生背景以及从原材料、反应历程、分离技术、产品和包装方面与传统方法的差别,指山了今后绿色化学的发展趋势。     

Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete chrysosporium

The present work studied the influence of glucose feeding on the ligninolytic enzyme production of Phanerochaete chrysosporium in a nitrogen-limited (C/N ratio is 56/8.8 mmol/L) medium. Several sets of shaking flask experiments were conducted. The results showed that 2 g/L glucose feeding on the first day of the culture (24 h after the inoculation) simulated both fungal biomass growth and enzyme production. The manganese peroxidase (MnP) activity was 2.5 times greater than that produced in cultures without glucose feeding. Furthermore, the glucose feeding mode in fed-batch culture was also investigated. Compared to cultures with glucose feeding every 48 h, cultures with glucose feeding of 1.5 g/L (final concentration) every 24 h produced more enzymes. The peak and total yield of MnP activity were 2.7 and 3 times greater compared to the contrast culture, respectively, and the enzyme was kept stable for 4 days with an activity of over 200 U/L. Translated from Acta Scientise Circumstantiae, 2007, 27(3): 363–368 [译自: 环境科学学报]     

加强高校校园文化建设促进学生的全面成才与发展

建设高校校园文化是发挥高校育人作用的重要工作,对全面推进素质教育和引导学生的全面发展都起着重要作用.本文就高校校园文化的概念、高校校园文化的作用、高校校园文化的建设要求与方法进行了论述,并提出了个人见解.     

室内培养底栖端足类日本大螯蜚饵料研究

本文在实验条件下探讨了５种饵料对海洋底栖端足类日本大螯蜚的培养效果。实验指标为存活率，生长速度，日增长率和发育状况。结果表明，青岛大扁藻与新月菱形藻１：１混合液的培养效果最佳，合成饲料是较好的补充饵料，螺旋藻粉不宜作为日本大螯蜚的培养饵料。动物性饵料培养效果不如植物性饵料，但对日本大螯蜚的早期快速成长具有一定的促进作用。     

影响微生物絮凝剂产生的因素研究

本文通过改变培养基的种类、培养基的碳源、氮源、无机盐离子来选择絮凝剂产生菌“Dfjm-1”高效低廉的培养基 ;通过改变培养基初始 p H值、培养温度、培养过程中的通气量等因素 ,得出“Dfjm-1”菌株产生絮凝剂的最佳条件。Dfjm-1产生絮凝剂的最佳因素为 :培养基初始 p H值为 6.5～ 7.0 ,培养温度为 3 0℃ ,培养时间为 60小时左右 ,通气量为 :早期 :2 50 r/ min;中期 1 50 r/ min;后期 1 0 0～ 1 50 r/ min     

Chorionic villus culture for prenatal diagnosis of chromosome defects: Reduction of the long-term cultivation time

We report in detail two series of chorionic villus cultivation for prenatal chromosomal diagnosis. Chorionic villi were sampled from both first- and second-trimester pregnancies. One hundred cultures were treated with trypsin–EDTA for 2 h and collagenase overnight, (method A) and 100 were treated with trypsin–EDTA for 1 h and collagenase for 2 h (method B). Using short-term enzymatic digestion, the cultivation time was reduced from 14 days to 6 days. Sufficient amounts of metaphases of good quality were present in 93 per cent of primary cultures harvested in situ, whereas enough metaphases of sufficiently good quality were in most cases present only after subcultivation of the cultures using method A. The decrease in cultivation time obtained is probably due to a higher yield of viable cells in monocellular suspension, an increased attachment efficiency, and a more rapid attachment of single cells (within 24 h).     

In vitro characteristics of human fetal cells obtained from chorionic villus sampling and amniocentesis

In vitro characteristics of human fetal cells have been investigated after chorionic villus sampling at the first trimester and amniocentesis at the second trimester of pregnancy. Light microscopy revealed heterogeneous morphology of cell types in both the chorionic villus culture and the amniotic fluid cultures. Based on the experiments performed, chorionic villus cells are more sensitive to pronase, trypsin, and versene during subculture and have a higher DNA content per single cell and release more [¹²⁵I]-Beta-human chorionic gonadotropin into culture medium than those found in amniotic fluid cells. The practical applications of this study are discussed.     

基于原代培养背角无齿蚌鳃细胞的镉毒性效应评价

为了探究淡水贝类背角无齿蚌鳃细胞的原代培养途径，并阐释其在评价水体Cd²⁺毒性效应上的潜力，本研究比较了不同酶分解方法（链霉蛋白酶、胰蛋白酶）的鳃细胞存活率差异，并分析了L-15培养基中不同血清浓度（10% FBS、20% FBS）对鳃细胞存活率的影响，细胞置于20℃生化培养箱中培养.进而根据Cd²⁺对鳃细胞的LC₂₅值设定0.0625、0.125、0.25、0.5和1.0 mg·L^-1 5个Cd²⁺理论浓度梯度，对原代培养的鳃细胞进行24 h暴露，分析了细胞活力、总超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和酸性磷酸酶（AcP）的变化特征.结果表明：0.025%链霉蛋白酶在4℃分解16 h的鳃细胞存活率为98.2%±0.2%，显著高于0.25%胰蛋白酶在26℃分解30 min的89.4%±3.5%鳃细胞存活率（p<0.05）；L15培养基加入10% FBS的细胞存活率总体显著高于添加20% FBS的细胞存活率（p<0.05）.在上述较佳的分解和血清浓度组合条件下，细胞培养120 h后，其存活率仍高达90.1%±4.7%.鳃细胞活力随着Cd²⁺浓度的增加而降低，两者之间呈显著的线性负相关（p<0.05）；随着Cd²⁺浓度的增加，SOD和AcP含量总体增加，而CAT含量呈现出"诱导-抑制"的变化趋势.本研究初步建立了较为适宜的背角无齿蚌鳃细胞的原代培养方法，并揭示了其原代培养鳃细胞的细胞活力及SOD、CAT、AcP水平，具有作为评价水环境Cd²⁺毒性/污染的生物指标的潜力.