聚糖原菌富集实验及其内源过程探究

细胞衰减是微生物内源过程的一个重要组成部分,可分为由细胞死亡引起的数量衰减和由细胞活性降低引起的活性衰减两部分.通过挥发性脂肪酸（VFA）吸收速率(VFAUR)测定、荧光原位杂交技术（FISH）以及LIVE/DEAD细胞染色技术,研究了富集聚糖原菌（GAOs）在序批式反应器（SBR）系统中好氧环境下的衰减特征.结果表明,当T=30℃、进料中m(COD)∶m(P)=100时,SBR系统中GAOs富集比例达94%.测定和计算表明,SBR富集系统中GAOs衰减速率和死亡速率分别为0.132 d^-1和0.034 d^-1,其数量衰减和活性衰减占其细胞总衰减比例分别为26%和74%.可见,GAOs数量衰减只占其细胞总衰减中很小一部分,而绝大部分衰减由活性衰减所引起.     

珠海市民及高校学生对垃圾分类和减废的认识和参与调查比较研究

珠海市在垃圾分类和减废相关政策的执行过程中,市民的意识和参与起着至关重要的作用。本研究对珠海市民及高校学生有关垃圾分类和减废的观念和行为进行问卷调查,对垃圾分类和减废的现状和困难进行了分析、比较和探讨,并对问题的解决提出了建议。     

20世纪云南省人员震亡基本特征分析

系统收集、整理了云南省人员震亡资料;在对资料可用性分析的基础上,以资料较为丰富的20世纪作为研究重点,分析了云南省人员震亡的时空分布,探讨了震亡与震级、烈度关系,总结了20世纪云南省人员震亡4个方面的基本特征,可供认识云南省下一轮强震活跃期人员的震亡情况参考。     

基于动态毒性负荷的人员疏散安全评估方法

毒气泄漏事故在高含硫气田及化工园区频繁发生,一旦发生事故,周边公众迅速进行疏散是应急的首要任务。我国很多地区制定了疏散方案,但是如何评估疏散的安全性和可行性,是亟待解决的问题。分析了我国泄漏事故特点和周边居民情况,对以往评估方法缺陷进行了剖析,提出基于动态毒性负荷分析的安全疏散评估方法。该方法在毒气扩散的精确3d模拟及疏散模拟的基础上,用差分法对人员行走过程中的毒性负荷Pc进行的计算,最终得出相应的致死概率Pr,并通过一个实例验证该方法的可行性。结果表明,运用该方法能够详尽、准确规划疏散区域、搬迁区域和避难区域。     

一种毒气事故避难所效果评估方法

目前我国针对公众防护只有疏散这一单一方法,现实情况下往往疏散不利导致大量人员伤亡,而同时避难策略由于缺乏理论和评估依据,往往难以推广。为了更好的全面保障毒气泄漏事故周边居民的生命安全,提出一套避难场所的效果评估方法。该方法主要包括房屋气密性测试、屋外浓度场计算、屋内浓度场计算分析、屋内致死概率分析。并举出实际应用案例,展现该方法的多种数据支撑作用。最终给出该方法对于制定避难策略过程中关键影响因素。该方法的提出为高含硫气田、化工园区周边公众防护策略的实施,提高其周边居民安全具有重要意义。     

大气细颗粒物控制对我国城市居民期望寿命的影响

收集我国2013年74个环保重点城市的细颗粒物(PM2.5)浓度.采用《全球疾病负担2010》提供的PM2.5与4种主要疾病死亡率的暴露反应关系系数,构建74个城市的城区居民寿命表,评估PM2.5造成的期望寿命损失和不同PM2.5控制目标对居民期望寿命的影响.结果显示,2013年我国74个城市的PM2.5污染可使居民期望寿命减少1.48岁;如果PM2.5年均水平降低10%、25%,可分别使期望寿命增加0.05岁和0.15岁;若进一步降低,达到国二标准、国一标准和世界卫生组织的空气质量指导值水平,可使期望寿命分别增加0.42岁、1.04岁和1.26岁.本研究提示,大气PM2.5污染是决定我国城市居民期望寿命的重要因素,降低PM2.5浓度可显著增加居民的期望寿命.     

A guide for assessing control room operator performance using speed and accuracy,perceived workload,situation awareness,and eye tracking

In the petrochemical industry, control room operators must address safety-critical alarms and other tasks using complex interfaces. This study developed a guide for assessing human performance using standard human factors measurement tools, and tested the sensitivity of those tools with two interface designs (i.e., gray and black) at three levels of workload (i.e., easy, medium, and difficult). The guide measures human performance through speed and accuracy, perceived workload using two standard instruments (i.e., NASA Task Load Index (NASA-TLX) and Subjective Workload Assessment Technique (SWAT)), situation awareness through the Situation Awareness Global Assessment Technique (SAGAT), and gaze through eye tracking coordinates. Twelve engineering student participants completed one simulation session at each of the three workload levels using one of two interface designs. Workload was manipulated through the number of simulated events (failures) in each session. Overall, the speed and accuracy measures, workload ratings, and eye tracking showed sensitivity to differences in workload level, and situation awareness showed sensitivity to the interaction between workload level and interface type. None of the tools were sensitive to interface type alone. Accuracy was highest under easy workload. Time per failure decreased at higher workload levels. Perceived workload ratings from the SWAT increased as workload increased, but workload ratings from the NASA-TLX were not different across workload levels. When workload increased, situation awareness remained steady for the gray interface but decreased sharply for the black interface, illustrating an interaction effect. Finally, the percentage of time spent looking at different areas of the screen during steady-state periods differed among workload levels. The tools in this guide can be used in the petrochemical industry to make design decisions for control room interfaces when workload levels are a concern.     

How Aware Are Other Nations of Climate Change? Analyzing Germans’ Second-Order Climate Change Beliefs About Chinese,US American and German People

ABSTRACT

What people believe about the beliefs of other people – second-order beliefs – has been acknowledged as a key factor that shapes public support for international climate policy. However, very little is known about their origins. Based on data from an online survey (n?=?935), we analyzed how German citizens assess the climate change awareness in their own nation as compared to those of the US and China. Even if the public climate change awareness in the US and China factually differs, we found that German citizens equivalently rate both nations similar and much lower than their own, a finding which can be explained with social identity processes and “in-group”/“out-group” biases. Hierarchical regression analyses demonstrate that the attention individuals pay towards television and social media predict second-order beliefs on climate change awareness positively, while attention to print media is a negative predictor.     

《大气污染防治行动计划》实施的环境健康效果评估

为了定量评估《大气十条》实施带来的环境健康效益,本文首先依据PM_(2.5)浓度和人口数据,分析2013～2017年全国人口加权浓度的变化,其次利用Ben MAP模型对全国338个地级及以上城市2013年PM_(2.5)基准情景和2017年PM_(2.5)控制情景进行分析,定量分析全国31个省(市)及338个地级及以上城市减少过早死亡人数.结果表明,由于PM_(2.5)浓度大幅下降,2013～2017年全国PM_(2.5)人口加权浓度呈逐年下降趋势;北京、天津、河北等京津冀及周边地区减少过早死亡人数最多. 2017年全国280个城市避免过早死亡人数有所增加,58个城市避免过早死亡人数有所下降.以WHO过渡期第1阶段目标值(PM_(2.5)年均浓度为35μg·m-3)作为控制情景,估算2013年全国过早死亡人数约为101 293人,2017年约为41 080人,《大气十条》的实施大约避免60 213人过早死亡.依据支付意愿法调查结果,估算增加的健康效益约为549. 7亿元.     

Programmed cell death induced by modified clay in controlling Prorocentrum donghaiense bloom

Modified clay (MC), an effective material used for the emergency elimination of algal blooms, can rapidly reduce the biomass of harmful algal blooms (HABs) via flocculation. After that, MC can still control bloom population through indirect effects such as oxidative stress, which was initially proposed to be related to programmed cell death (PCD) at molecular level. To further study the MC induced cell death in residual bloom organisms, especially identifying PCD process, we studied the physiological state of the residual Prorocentrum donghaiense. The experimental results showed that flocculation changed the physiological state of the residual cells, as evidenced by growth inhibition and increased reactive oxygen species production. Moreover, this research provides biochemical and ultrastructural evidence showing that MC induces PCD in P. donghaiense. Nuclear changes were observed, and increased caspase-like activity, externalization of phosphatidylserine and DNA fragmentation were detected in MC-treated groups and quantified. And the mitochondrial apoptosis pathway was activated in both MC-treated groups. Besides, the features of MC-induced PCD in a unicellular organism were summarized and its concentration dependent manner was proved. All our preliminary results elucidate the mechanism through which MC can further control HABs by inducing PCD and suggest a promising application of PCD in bloom control.