DNA polymorphism and globin chain analysis in the prenatal diagnosis of β-thalassaemia major in Taiwan

Thirty-six pregnancies in 25 families at risk of β-thalassaemia major received prenatal diagnosis. Chorionic villus sampling or amniocentesis was done in 35 pregnancies to obtain fetal cells for DNA linkage study, for which Southern blotting and DNA hybridization were used to detect seven restriction fragment length polymorphisms (RFLPs) within the β-globin gene cluster: ϵ-HincII, ^Gγ-HindIII, ^Aγ-HindIII, Φβ-HincII, 3′Φβ-HincII, β-AvaII, and 3′β BarnHI. β-Thalassaemia major was diagnosed in seven and excluded in 22 pregnancies. In the remaining six cases, β-thalassaemia major could not be excluded. In these six pregnancies and another one with late booking, ultrasound-guided cordocentesis was performed at the 22nd to 27th week of gestation. Globin chain composition was determined with urea-acetic acid-Triton X-100-12 per cent polyacrylamide gel electrophoresis. β-Thalassaemia major was diagnosed in two fetuses and excluded in the other five. Eleven fetuses (in which β-thalassaemia major was excluded) have been delivered and are healthy at more than 5 months old DNA linkage analysis coupled with globin chain electrophoresis provides an effective way for prenatal diagnosis of β-thalassaemia major, although these methods are being replaced by more direct detection techniques using oligonucleotide probes.     

宁夏人口、粮食与水土资源利用

人口、粮食与水土资源利用是宁夏回族自治区国民经济发展中的重要问题。文中分析了宁夏人口、粮食与水土资源利用现状,应用灰色系统理论及回归分析,建立人口、粮食预测模型,对2000年宁夏的人口、粮食进行了预测。分析2000年水土资源不同开发利用条件下粮食供需平衡情况后指出,充分合理利用黄河水资源,扩大灌溉面积,配合先进的农业技术,宁夏粮食不仅能自给,而且还能提供一定数量的商品粮。最后提出了解决粮食问题,防止生态环境恶化的主要途径。     

加氢原料缓冲罐串压事故动态模拟研究

通过工艺流程动态模拟,研究某渣油加氢装置在反应进料泵事故紧急停车后,不同工艺设计方案组合条件下,引发高压气体反串到低压原料缓冲罐的事故影响。研究结果表明:泵出口双单向阀和紧急切断阀的设计对降低超压事故的发生有很好的保护作用;切断阀关闭时间越短对原料缓冲罐超压保护越有利;安全阀和缓冲罐的设计条件须与切断阀关闭时间和单向阀数量等设计条件综合考虑。通过动态模拟方法研究工艺操作异常工况,对优化工艺设计,提高本质安全设计有参考价值。     

冷挤压工艺智能设计中的压力计算

冷挤压力的高低是冷挤压工艺成败的关键 ,通常挤压力计算是人工完成的。文中介绍了冷挤压工艺计算机智能设计系统中的挤压力计算及其参数优化部分的软件开发方法。该方法分别介绍了数据库设计、用户界面设计、挤压力计算、工艺评价等内容     

山西运城盆地多次大面积高频地振动事件的调查

本文对山西运城遥测地震台网及山西省数字流动地震观测仪记录的2005年1月31日至2月28日在山西省运城盆地范围内发生的7次高频地振动波形进行分析处理，精确划出了振动范围，并结合本区域内的地质构造分析震情趋势。     

冷轧带钢轧辊温度场及其控制

分析了冷轧过程中工作辊的温度变化及热凸度的形成 ,提出了控制热凸度的有效手段 ,对完善板型控制系统具有重要的意义     

中国矿产资源持续开发与区域开发战略调整

为完成预定的工业化发展目标,21世纪中国矿产资源的消费总量和结构将呈现持续增长和重大转变。从矿产资源持续开发的角度出发,中国不仅努力加强自身供应保障能力的建设,而且也要努力进行资源开发的区域开发调整。考虑到有限的矿产资源基础和脆弱的生态环境,中国矿产资源持续开发的基本模式可以考虑为:结构改善+技术进步+资源国际化+环保意识增强。相应地,区域开发调整模式则为:充分开放东部,积极稳定中部,适度开发西部。     

冷挤大型法兰面螺母的工艺研究

以大型法兰面螺母为例 ,论述了用无缝钢管冷挤大型法兰面螺母工艺的可行性     

Detection of fetal HLA-DQα sequences in maternal blood: A gender-independent technique of fetal cell identification

The objective of this study was to detect fetal HLA-DQα gene sequences in maternal blood. HLA-DQα genotypes of 70 pregnant women and their partners were determined for type A1. We specifically sought couples where the father, but not the mother, had genotype A1. In 12 women, maternal blood samples were flow-sorted. Candidate fetal cells were isolated and amplified by using PCR primers specific for a paternal HLA-DQα A1 allele. Fetal HLA-DQα A1 genotype was predicted from sorted cells; amniocytes or cheek swabs were used for confirmation. Six of twelve sorted samples had amplification products indicating the presence of the HLA-DQα A1 allele; 6/12 did not. Prediction of the fetal genotype was 100 per cent correct, as determined by subsequent amplification of amniocytes or cheek swabs. We conclude that paternally inherited uniquely fetal HLA-DQα gene sequences can be identified in maternal blood. This system permits the identification of fetal cells independent of fetal gender, and has the potential for non-invasive prenatal diagnosis of paternally inherited conditions.     

九江-瑞昌5.7级地震现场房屋安全性鉴定工作的探讨

九江-瑞昌地震虽然震级较小,但震源深度浅,加之本地为历史少震地区,设防水准低,量大面广的非标准砖混和砖木房屋破坏较严重,造成13人死亡和多达20亿的直接经济损失.这充分暴露出我国少震地区城市防御力不足的弊端,经济发展的同时更应注意地震灾害风险的规避.本文介绍了本次地震现场应急工作情况,例举了地震区内不同结构类型房屋在这次地震中的表现和震害特点,并根据本次地震现场房屋安全性鉴定工作的实践和多次地震现场应急工作的经验,对本次地震现场应急工作进行总结.