全文获取类型
收费全文 | 1616篇 |
免费 | 25篇 |
国内免费 | 11篇 |
专业分类
安全科学 | 65篇 |
废物处理 | 3篇 |
环保管理 | 4篇 |
综合类 | 1529篇 |
基础理论 | 13篇 |
污染及防治 | 5篇 |
评价与监测 | 9篇 |
社会与环境 | 8篇 |
灾害及防治 | 16篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 10篇 |
2021年 | 16篇 |
2020年 | 6篇 |
2019年 | 7篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 7篇 |
2013年 | 10篇 |
2012年 | 21篇 |
2011年 | 65篇 |
2010年 | 58篇 |
2009年 | 44篇 |
2008年 | 58篇 |
2007年 | 80篇 |
2006年 | 87篇 |
2005年 | 84篇 |
2004年 | 90篇 |
2003年 | 83篇 |
2002年 | 94篇 |
2001年 | 75篇 |
2000年 | 2篇 |
1999年 | 5篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1996年 | 3篇 |
1995年 | 84篇 |
1994年 | 75篇 |
1993年 | 73篇 |
1992年 | 57篇 |
1991年 | 57篇 |
1990年 | 51篇 |
1989年 | 61篇 |
1988年 | 47篇 |
1987年 | 50篇 |
1986年 | 29篇 |
1985年 | 42篇 |
1984年 | 44篇 |
1983年 | 21篇 |
1982年 | 21篇 |
1981年 | 16篇 |
排序方式: 共有1652条查询结果,搜索用时 15 毫秒
121.
122.
Elles M. J. Boon Hélène B. Schlecht Peter Martin Geoff Daniels Rolf H. A. M. Vossen Johan T. den Dunnen Bert Bakker Rob Elles 《黑龙江环境通报》2007,27(10):932-937
Objectives To validate the use of Real Time PCR, a widely used technique that can detect very low levels of Y chromosomal sequence, and to assess the use of a highly sensitive PCR technique, pyrophosphorolysis-activated polymerisation (PAP), for fetal sex determination using free fetal DNA (ffDNA). Methods The fetal sex was determined by Real Time PCR in 58 pregnancies using ffDNA isolated from maternal plasma. In parallel with the Real Time PCR experiments, the presence of Y chromosome sequence was also determined using PAP on 54 isolated ffDNA samples. Results Both techniques detected Y chromosome sequence at very low levels with 98% specificity and 100% sensitivity (Real Time n = 44, PAP n = 54). Furthermore, the PAP technique was shown to be more robust than the Real Time PCR as none of the samples tested failed to meet the acceptance criteria. Combining the two techniques for male fetal sex detection from maternal blood plasma increases the sensitivity and specificity to 100% in this series. Conclusions This study shows that both Real Time PCR and PAP can be used for Y chromosome detection on ffDNA. Furthermore, by using PAP in combination with Real Time PCR more reliable early prenatal sexing can be performed using ffDNA. Copyright © 2007 John Wiley & Sons, Ltd. 相似文献
123.
124.
Congenital limb deficiency (CLD) occurs in 0.54 to 0.59/1000 live-born infants and varies according to its anatomic location, type and cause. Our aim was to present a fetus with a prenatal ultrasonographic diagnosis of CLD (transverse reduction deficiency of the left upper limb), and to determine the prevalence and clinical characteristics of CLD at a tertiary medical centre in Israel. Among 78 500 live-born infants, there were 24 cases of CLD (0.31/1000): 45.8% of the CLD cases with affected upper limbs, 45.8% with affected lower limbs, and 8.4% with both limbs affected; 88.4% of the limb deficiencies were longitudinal and 11.6% were transverse; and, 33.3% (8/24) of the affected newborn infants had additional congenital anomalies. We conclude that CLD is not an infrequent finding in live-born infants. Comprehensive ultrasonography of the fetus allows early prenatal diagnosis of CLD and provides the parents with important information and helps them in their decision regarding the fate of the pregnancy. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
125.
126.
We describe here a new type of method for isolation of rare cell populations in biological fluids. The method is based on the anthraquinone technology for covalent binding of molecules to a polymer surface. An anthraquinone molecule conjugated via a linker to an electrophilic group (AQ Immobilizer™ reagent, Exiqon A/S) is covalently bound to a polymer surface by UV irradiation. The electrophilic group of this AQ reagent can covalently bind a specific antibody directed against a specific cell marker. Applying a cell sample to the functional surface, the cells having the specific cell marker on the cell surface will bind to the antibody on the functional surface. Using this technique, even extremely small cell populations may be isolated. We succeeded in isolating fetal cells from maternal blood samples in the first trimester for chromosome defects genetic diagnosis. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
127.
E. Marchina G. Piovani L. Vezzola D. Bellotti V. Cerri C. Groli Prof. S. Barlati 《黑龙江环境通报》2003,23(12):959-963
A 40-year-old woman underwent amniocentesis at 15.3 weeks of gestation. Chromosome analysis performed using QFQ, DA-DAPI and CBG banding revealed two de novo extra-chromosomal markers (ESACs) in 11 of the 16 colonies analysed. Fluorescence in situ hybridization (FISH) showed that both chromosomes came from the Yq11.22.1 region of the Y chromosome. PCR analysis of genes and STS localized on the Y chromosome excluded the Yp presence specifically of the SRY gene, and most of the euchromatic region of Yq. After extensive genetic counselling and considering both laboratory and second-level ultrasound data, the couple decided to continue the pregnancy. At 37.4 weeks of gestational age, a girl weighing 2750 g was born with an Apgar score of 9/10. A blood sample taken from the umbilical cord showed three cellular lines:mos47,XX, +mar1 ish.der (Y)(wcpY+) [21%]/48,XX, +mar1 ish.der (Y)(wcpY+), +mar2 ish.der (Y)(wcpY+) [41%]/46,XX [38%]. One year after birth, the baby was developing normally and had normal psychomotorial activity. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
128.
129.
130.