Exoautometabolic regulation of transgenes in microorganisms

Data on the existence of a specific system regulating the growth, size, and structure of enterobacterial populations are first summarized in terms of the chemical ecology concept formulated by S.S. Shvarts. The role of this system in transgene functioning is described using the example of the lux operon integrated into Escherichia coli. A relatively simple test for estimating such interactions is proposed.Translated from Ekologiya, No. 2, 2005, pp. 91–96.Original Russian Text Copyright © 2005 by Pshenichnov, Maslennikova, Nikitina.     

A comparison of different lysis buffers to assess allele dropout from single cells for preimplantation genetic diagnosis

Single cell polymerase chain reaction (PCR) for preimplantation genetic diagnosis (PGD) requires high efficiency and accuracy. Allele dropout (ADO), the random amplification failure of one of the two parental alleles, remains the most significant problem in PCR-based PGD testing since it can result in serious misdiagnosis for compound heterozygous or autosomal dominant conditions. A number of different strategies (including the use of lysis buffers to break down the cell and make the DNA accessible) have been employed to combat ADO with varying degrees of success, yet there is still no consensus among PGD centres over which lysis buffer should be used (ESHRE PGD Consortium, 1999 ). To address this issue, PCR amplification of three genes (CFTR, LAMA3 and PKP1) at different chromosomal loci was investigated. Single lymphocytes from individuals heterozygous for mutations within each of the three genes were collected and lysed in either alkaline lysis buffer (ALB) or proteinase K/SDS lysis buffer (PK). PCR amplification efficiencies were comparable between alkaline lysis and proteinase K lysis for PCR products spanning each of the three mutated loci (ΔF508 in CFTR 90% vs 88%; R650X in LAMA3 82% vs 78%; and Y71X in PKP1 91% vs 87%). While there was no appreciable difference between ADO rates between the two lysis buffers for the LAMA3 PCR product (25% vs 26%), there were significant differences in ADO rates between ALB and PK for the CFTR PCR product (0% vs 23%) and the PKP1 PCR product (8% vs 56%). Based on these results, we are currently using ALB in preference to PK/SDS buffer for the lysis of cells in clinical PGD. Copyright © 2001 John Wiley & Sons, Ltd.     

Tropic attributes of the mudskipper, Periophthalmus barbarus (Gobiidae: Oxudercinae) in the mangrove swamps of Imo River estuary, Nigeria

Aspects of the trophic attributes of the mudskipper,Periophthalmus barbarus occurring in the mangrove swamps of ImoRiver estuary, Nigeria, were investigated during a 12-month period(April, 1992-March, 1993). Feeding intensity increased with size;it was higher in the dry season (November-April) than in the wetseason (May-October); monthly changes in the indices of feedingintensity were significantly correlated. Major food objectscomprised detritus and benthic algae; crustaceans, insects,macrophyte matter and sand grains were minor food objects whilefish, molluscs, Chilopoda, nematodes and fungi were unconsciouslydevoured objects. Diet varied seasonally; algae and sesarmid crabswere generally more in the dry season than during the rains whereasFPOM and fish increased in the wet over dry season. The mudskipperdisplay a high degree of euryphagy and can be considered as ageneralist and an omnivore. The lower trophic status of P. barbarusqualifies it as a good aquaculture candidate. Food richness washigher in big-sized class (BSC) than small-sized class (SSC).Size-based and seasonal variations were not apparent in foodrichness. Diet breadth was higher in the BSC than the SSC; therewas dry season increase in diet breadth.     

江汉平原粮食开发必须走优质高产高效的道路

分析江汉平原粮食生产的优势与问题,运用商品经济的观念,提出改革开放新形势下加快江汉平原粮食开发的设想,即依托市场,按照价值规律,科学地调整种植业比例和粮食种类结构,发展优质品种,扩大优化模式及采用高新技术等,通过优质、高产、高效,促进江汉平原粮食生产的发展。     

中国可持续社会养老保险的综合评价体系和实证分析

我国可持续社会养老保险的综合评价指标体系包括四个层次和三级子系统，内容涉及养老保险人数、机构设置、基金管理和代际平衡四个子目标。应用因子分析模糊综合评价方法对我国1990-2001年养老保险建设状况进行评价，本文认为基金管理，行政管理和代际失衡是影响我国可持续养老保险发展的主要因素。     

Toxicological Assessment of Wastewater and Runoff from the Baikal Pulp and Paper Mill

Russian Journal of Ecology -     

Physicochemical aspects of using natural scavengers in removing methylene blue (basic dye) from solution

Effluent from dyeing and finishing processes is an important source of water pollution. The effectiveness of bentonite, kaolinite and sediment from a local deposit in removing methylene blue as a cationic dye from aqueous solutions has been investigated. The adsorption equilibrium (isotherm) has been determined according to Freundlich and Langmuir equations. The optimum amount is 0.5 g for all adsorbents, and the optimum pH ranges are 2-8 for bentonite and 2-6 for kaolinite and sediment. With respect to kinetic modelling, the adsorption of methylene blue on various adsorbents was fitted to a second-order equation. Also, the thermodynamic parameters were determined. The negative free energy values indicate the feasibility of the process and spontaneous nature of adsorption. The positive ΔH° values indicate the endothermic nature of the process. Thus, Egyptian clay minerals and sediments have a great tendency to remove the dye from solutions.     

有意违章行为动因分析与控制对策探讨

对有意违章行为的含义进行了界定 ,并对有意违章行为产生的动因进行了详细分析 ,提出了有意违章行为的动因主要包含违章行为与遵章行为满足生理心理需要作用的差值ΔXW、违章行为会受到法规惩处的作用FW、违章行为可导致人身伤害的主观感觉的作用RW3个方面。同时对 3个方面的动因按弗鲁姆的激励理论进行了转化 ,给出了各自的含义说明。笔者按动因分析的结果 ,给出了控制有意违章行为的步骤和措施     

Mangrove Mapping Using Landsat Imagery and Aerial Photographs: Kemaman District, Terengganu, Malaysia

Classification and distribution of mangrove vegetation are vital information for the proper development of a mangrove management plan. In this study, classification for the mangroves of the district of Kemaman were done using both 1 : 5000 aerial photographs and Landsat TM imageries. The coverage by aerial photographs is limited to the coastal and estuarine areas only. Thus, for areas further upstream of the aerial photo coverage, Landsat TM imageries were used. Analysis of aerial photographs and remote sensing images revealed that the mangroves of Kemaman could be classified into 14 different classes of vegetation. All the 14 classes were identified from areas covered by the aerial photographs. For areas covered by the Landsat images only 7 classes of vegetation were identified. The accuracy for aerial photograph and Landsat images are 91.2% and 87.8%, respectively. It can be concluded that although both techniques are useful in determining the mangrove vegetation classes, the large 1 : 5000 aerial photographs are more accurate and provided more detailed information comparatively.