Rapid prenatal diagnosis of aneuploidy from uncultured amniotic fluid cells using five-colour fluorescence in situ hybridization

In this paper we describe the use of five-colour fluorescence in situ hybridization for prenatal diagnosis of aneuploidy using uncultured amniotic fluid cells. The analysis is based on ratio mixing of dual-labelled probes and digital imaging for the detection and visualization of five different probes specific for the five target chromosomes, 13, 18, 21, X, and Y. A retrospective blind analysis of 30 coded uncultured amniotic fluid samples correctly detected fetal sex and five trisomy 21 cases. Multicolour fluorescence in situ hybridization used in this way allows rapid and simultaneous detection of the most frequent aneuploidies.     

水泥土桩复合地基桩土应力参数研究

本文首先通过理论分析，认为桩土应力比、桩土荷载分担比等是反映水泥土桩复合地基工作性状的重要参数。然后通过现场试验，研究桩土应力比、桩土荷载分担比随荷载变化的规律。结果认为：随着荷载增加，桩土应力比逐渐减小，当比值达到某一特征值后，不再发生明显变化；桩土应力比、桩土荷载分担比不为常数，其变化是桩土变形协调的结果。最后得出有一定参考价值的结论。     

A值法研究大气总量控制的环境质量达标保证率

在总量控制区用A值法实行总量控制后，总量控制区在符合污染物年允许排放总量限值时，污染物的日平均浓度和小时平均浓度不一定能达到国家或地区大气环境质量标准。为了反映总量控制区环境质量达标情况，本文提出了A值法研究大气总量控制的环境质量达标保证率的概念，通过总量控制A值法的基本原理，给出小时／日环境质量达标保征率的量化公式，经过分析得到；环境质量达标保证率与污染物种类有关，同一污染物的保证率与A值的年分布情况密切相关。同时利用长江下游某地的2000年气象资料分析该地区A值的变化趋势，求得该地区SO2和N02的小时保征率分别为97．4％、90.2％，日保征率分别为90.2％、79．5％。     

强震仪观测资料在杨浦大桥养护监测中的运用

上海杨浦大桥是当时世界同类型桥梁中最长的斜拉索桥,为了掌握大桥的运行情况,确保运行安全,每年用在该桥上的养护管理费用相当可观,且程序繁琐。如何找到既简单实用,又节约费用,就能起到一定的养护监测作用的方法?是本文探讨的问题。本文运用强震观测资料,采用自回归移动平均ARMA模型,得到大桥的随机振动中某些振型的频率及阻尼比,在地震、台风过后或经一长段时间车辆运行之后,看其相应振型频率和阻尼比的变化,以达到维护监测的目的。从所得的结果看,此法具有较好的可操作性,可用于大桥主塔等的养护。     

我院电子阅览室利用情况分析

分别按专业、系部、年级、性别等方面统计了学生的开户情况，在不同的时间段调查了学生上机的目的，然后对学生在电子阅览室的开户情况和上机行为进行了深入分析，在此基础上，对我院电子阅览室的管理提出了几点建议。     

枇杷核中提取苦杏仁甙的研究

研究了3个枇杷品种的出核率，利用高效液相色谱检测苦杏仁甙的含量，以及从枇杷核中提取苦杏仁甙的工艺。结果表明，3个品种烘干后核的得率存在显著差异，干核的年产量很大，有综合开发利用价值；提取溶剂以乙醇为好，最合理的工艺组合为：在原料中按料液比1：10加入浓度为80％的乙醇，在65℃时回流提取50min。在40℃功率为80W的条件下超声处理25min。合理选择品种是提高苦杏仁甙得率的关键。     

Application of tracer ratio and inverse dispersion methods with boat-based plume measurements to estimate ammonia emissions from seabird colonies

Ammonia emissions from two contrasting seabird colonies in Scotland were measured, based on the determination of atmospheric concentrations downwind of the colonies. Atmospheric concentrations of ammonia (NH₃) across the downwind plume were compared with the inverse application of a Gaussian dispersion model (ID) to calculate the modelled NH₃ emission that would generate the measured cross-wind-integrated plume concentration. In parallel, a tracer gas (sulphur hexafluoride, SF₆) was released from the colonies with air samples taken to allow determination of SF₆ concentrations. On the basis of the known emission rate of SF₆, the magnitude of ammonia emissions was estimated by the cross-wind-integrated tracer ratio (TR) of NH₃/SF₆ concentrations. Coupled with data on annual bird attendance, the measurements indicate annual emissions from the Isle of May and the Bass Rock of 18 and 132 tonnes NH₃-N year^–1, respectively. The measured NH₃ emissions were compared with estimates of seabird nitrogen excretion to estimate the proportion of excreted N that is volatilised as NH₃ (F_Nr). The emission estimates of the two methods compared favourably, giving 4 and 6 kg NH₃-N h^–1 (F_Nr = 15%) for the Isle of May for the ID and TR methods, respectively, and 21 and 25 kg NH₃-N h^–1 (F_Nr = 50%) for the Bass Rock for the ID and TR methods, respectively. The results provide the first measurement-based estimates to allow regional up scaling of ammonia emissions from seabirds.     

近断层地震动考虑能量损伤效应的非弹性强度需求

由于靠近断层处地震地面运动中速度脉冲作用的存在,导致传统的基于瞬时加速度反应谱的抗震设计,不能有效考虑由此带来的巨大能量耗散和位移需求。笔者采用代表性的脉冲型实际近场地震记录和人工模拟三角函数地震动时程,在分析累积输入地震动耗能和结构位移间关系基础上,提出了等效速度比的表达式;给出了近场地震动的等效变形需求表达式;提出了等价延性系数的概念,来考虑由于地震动能量耗散对结构的强度需求;经非线性时程分析方法对其稳定性进行了验证比较,有利于增强结构的安全性。     

Experimental study on the synergistic inhibition effect of nitrogen and ultrafine water mist on gas explosion in a vented duct

In this study, in order to research the synergistic inhibition effect of nitrogen and ultrafine water mist on gas explosion in a vented duct, a semi-confined transparent chamber was designed with the size of 120 × 120 × 840 mm, and the experiments were carried out with stoichiometric methane/air premixed mixture (fraction of methane: 9.5%), adding different fractions of nitrogen and ultrafine water mist. The experimental results showed the following: The combination of nitrogen and ultrafine water mist had a synergistic inhibiting effect on methane/air explosion, which was preferable to the single use of any kind. With the increase of spraying time of water mist and fraction of nitrogen, the initial shape of the explosion flame became snakelike, and at the same time the peak flame propagation speed and peak overpressure decreased significantly. When the nitrogen fraction was increased to 10% and the mist spraying time was increased to 2min, synergistic inhibiting effect on overpressure was high efficient. However, with the increase of spraying time of water mist and fraction of nitrogen going on, the amount of increase of explosion inhibition efficiency was gradually reduced.     

Experimental study on explosion inhibition by heptafluoropropane and its synergy with inert gas

The inhibition effect of heptafluoropropane (CF₃CHFCF₃) on methane explosions under different inhibitor concentrations in a closed vessel was studied. A high-speed camera and a pressure sensor were adopted respectively to record flame propagation characteristics and pressure data. Results indicate that the relationship between flame propagation and pressure rising was correlated. As the equivalent ratio (ϕ)≤1, the pressure presented a trend of rising firstly and then decreasing with increasing CF₃CHFCF₃ concentration, and it was found that there existed a critical concentration for pressure decrease. As ϕ > 1, the pressure exhibited a decreasing trend. Although the pressure appeared to seemingly increase, the moment that the pressure began to rise (t_rise) and the moment that the maximum explosion overpressure appeared (t_Pmax) were obviously delayed. The average rate of pressure rise ((dP/dt)_ave) was decreased as the concentration of CF3CHFCF3 increased. It indicates that CF₃CHFCF₃ can effectively reduce the explosion reaction rate. The critical concentration of CF₃CHFCF₃ for complete inhibition was determined. Meanwhile, the synergy of CF₃CHFCF₃-inert gas can improve the inhibition effect. Compared with CF₃CHFCF₃–N₂, the synergy of CF₃CHFCF₃–CO₂ presented a better inhibition effect, and the inhibition effect was increased with increasing inert gas concentration. And the mechanisms of physical and chemical effects on explosion inhibition were analyzed.