Chorionic villus culture for prenatal diagnosis of chromosome defects: Reduction of the long-term cultivation time

We report in detail two series of chorionic villus cultivation for prenatal chromosomal diagnosis. Chorionic villi were sampled from both first- and second-trimester pregnancies. One hundred cultures were treated with trypsin–EDTA for 2 h and collagenase overnight, (method A) and 100 were treated with trypsin–EDTA for 1 h and collagenase for 2 h (method B). Using short-term enzymatic digestion, the cultivation time was reduced from 14 days to 6 days. Sufficient amounts of metaphases of good quality were present in 93 per cent of primary cultures harvested in situ, whereas enough metaphases of sufficiently good quality were in most cases present only after subcultivation of the cultures using method A. The decrease in cultivation time obtained is probably due to a higher yield of viable cells in monocellular suspension, an increased attachment efficiency, and a more rapid attachment of single cells (within 24 h).     

金堆城超大型钼矿床水-岩δD-δ~(18)O同位素交换体系理论模型及成矿流体来源

基于水-岩δD-δ18O同位素交换体系理论模型的建立和计算，对金堆城超大型斑岩钼矿四个不同成矿期中成矿流体的演化和来源进行了研究，发现在成矿前期和早期，成矿流体为岩浆水与围岩在较低水／岩比值（0．1W／R＞0．001）和中、高温（t=250～500℃）条件下反应后的残余流体，而主成矿期和成矿晚期的成矿流作为大气降水在相对较高水／岩比值（0．5＞W／R0．1）和中、低温（t＝150～310℃）条件下与围岩反应后的残留流体。大气降水在金堆城钼矿成矿过程中起了重要作用。在主成矿期水／岩比值达到最高。     

Maternal sex chromosome mosaicism diagnosed by amniocentesis and percutaneous umbilical cord sampling

We present a remarkable chain of events in which percutaneous umbilical cord sampling was performed in an attempt to clarify a situation of possible fetal sex chromosome mosaicism in an amniotic fluid culture and led to the discovery that the mother herself had a 45,X/46,XX/ 47.XXX chromosome constitution. This may have simply represented the chance concurrence of pseudo-mosaicism in the amniotic fluid culture of a woman with an abnormal sex chromosome constitution, but it is also possible that the 45,X colony was maternal in origin. Although clearly a most unusual circumstance, the possibility should be kept in mind when termination of a pregnancy is being considered because of apparent mosaicism in a prenatal diagnostic study.     

抚仙湖面源污染特征及其防治对策

根据抚仙湖径流区污染物负荷量的调查结果,抚仙湖水污染特征以面源为主,面源入湖水量占98%,TP占81.4%,TN占85.9%,因此控制面源是保护抚仙湖的关键。提出了防治抚仙湖面源污染的生态治理及工程治理对策。     

滇池流域农村面源问题浅析

介绍了滇池流域自然和社会经济概况,分析了非点源污染的特性。认为滇池流域农村面源主要由村镇生活污水、农村固体废物、化肥施用、地表径流、土壤侵蚀和流失、畜禽养殖粪便构成。在得出结论的基础上提出了措施。     

地球环境中的放射性污染

地球环境中的放射性污染有天然辐射来源和人为放射性来源,通过消化道、呼吸道和皮肤、粘膜或伤口进入人体内,对人体形成直接损伤、间接损伤和远期辐射效应,应从内照射和外照射防护两个方面来防止环境中的放射性污染.     

精密模锻件的设计要点

讨论了精密模锻件的分类密集形状的锻件、盘形锻件和长杆形锻件 ,以及精密模锻件的设计要点精密模锻件的机械加工余量、尺寸精度和表面粗糙度、分模面的选择、模锻斜度、圆角半径、肋的设计和腹板厚度的设计     

Progress in the research on aquatic environmental nonpoint source pollution in China

ＰｒｏｇｒｅｓｉｎｔｈｅｒｅｓｅａｒｃｈｏｎａｑｕａｔｉｃｅｎｖｉｒｏｎｍｅｎｔａｌｎｏｎｐｏｉｎｔｓｏｕｒｃｅｐｏｌｕｔｉｏｎｉｎＣｈｉｎａＢａｏＱｕａｎｓｈｅｎｇ，ＭａｏＸｉａｎｑｉａｎｇ，ＷａｎｇＨｕａｄｏｎｇＩｎｓｔｉｔｕｔｅｏｆＥｎｖｉｒｏ...     

未熟-低熟烃源岩的有机岩石学特征

采用全岩光片镜鉴和显微荧光分析方法 ,对我国不同地区 5 0 0余块未熟 低熟烃源岩样品进行了系统的有机岩石学研究。未熟 低熟烃源岩显微组分组成具有显著的非均质性 ,不同地区显示出陆源组分与水生生源组分不同的“配比”关系 ,反映出其有机质组成的复杂性 ,并确认藻类体、孢子体、树脂体、木栓质体、壳屑体和矿物沥青基质等组分是其最常见的生烃组分。基于对镜质组反射率Ro和孢子体荧光变化等演化指标特征的分析 ,认为未熟 低熟烃源岩有机质早期热演化具有明显的阶段性 ,一般都表现为“二段式”变化 ,其变化界限大致以Ro等于 0 .5 0 %～ 0 .5 5 %为界。     

模糊数学对水源水质进行分析的应用实例

以某取水河道的某一次监测结果为例子,以GB 3838-2002《地表水环境质量标准》为依据,探讨运用模糊数学对水源水进行分析的方法,绘制圆形比例图,显示各种污染物对水源水质"贡献",从而列出风险污染物清单,以便在工程中采用适当的处理工艺.