利用ATP生物发光法快速检测丽娃河中细菌总数

利用ATP生物发光法对丽娃河中细菌总数进行了快速检测。结果表明,丽娃河中细菌总数维持在0.72×106～1.2×107CFU/mL,较综合整治前的水质有了明显地改善。研究了游离ATP以及浮游生物等对发光强度的影响,发现对样品采用过滤的方法进行预处理是提高ATP生物发光法精确度的一种重要手段。     

我国含盐废水生物处理的研究进展

综述了目前我国在含盐废水生物处理的研究进展,包括传统活性污泥法、SBR、生物接触氧化法、厌氧生物处理等,在不同的运行条件和处理状况下取得的较好的处理效果。并在此基础上介绍了耐盐细菌法和物化一生化组合工艺法两种非常有效的处理工艺。     

16S rDNA 克隆文库法分析Biostyr 曝气生物滤池处理城市污水的细菌多样性研究

采用分子生物学手段16SrDNA克隆文库方法,对第三代生物膜法代表工艺Biostyr曝气生物滤池（BAF）中滤料表面细菌进行了多样性研究.从16SrDNA克隆文库中随机挑选了50个克隆子进行序列测定（约1.5kb）,对测序结果进行了BLAST对比.结果表明,BiostyrBAF系统中的细菌群落具有高度多样性,有41个克...     

成熟锰砂表面细菌的分离及其氧化铁锰能力

从取自不同电厂成熟锰砂表面的活性滤膜中分离出7种细菌,经鉴定分别属于纤发菌属、球衣菌属、鞘铁细菌属.研究表明,这3个菌属的微生物对水中Fe2+都具有较好的去除效果,其中鞘铁细菌1号对铁的去除效果最好;纤发菌和鞘铁细菌对水中Mn2+都具有良好的去除效果,其中鞘铁细菌1号的去除能力最强,鞘铁细菌2号次之.将分别堵养的具有氧...     

Biodegradation of geosmin in drinking water by novel bacteria isolated from biologically active carbon

Three strains of Gram-negative bacteria capable of removing geosmin from drinking water were isolated from biologically active carbon and identified to be Chryseobacterium sp., Sinorhizobium sp. and Stenotrophomonas sp. based on physio-biochemistry analysis and 16S rRNA gene sequence analysis. Removal e ciencies of 2 mg/L geosmin in mineral salts medium were 84.0%, 80.2% and 74.4% for Chryseobacterium sp., Sinorhizobium sp. and Stenotrophomonas sp., respectively, while removal e ciencies of 560 ng/L geosmin in filter influent were 84.8%, 82.3% and 82.5%, respectively. The biodegradation of geosmin was determined to be a pseudo first-order reaction, with rate constants at 2 mg/L and 560 ng/L being 0.097 and 0.086 day??1, 0.089 and 0.084 day??1, 0.074 and 0.098 day??1 for the above mentioned degraders, respectively. The biomass of culture in the presence of geosmin was much higher than that in the absence of geosmin.     

Succession of bacterial community along with the removal of heavy crude oil pollutants by multiple biostimulation treatments in the Yellow River Delta, China

Multiple biostimulation treatments were applied to enhance the removal of heavy crude oil pollutants in the saline soil of Yellow River Delta. Changes of the soil bacterial community were monitored using the terminal restriction fragment length polymorphism (T-RFLP) and clone library analyses. The 140-day microcosm experiments showed that low C:N:P ratio, high availability of surfactant and addition of bulking agent significantly enhanced the performance, leading to the highest total petroleum hydrocarbon removal. Meanwhile, the bacterial community was remarkably changed by the multiple biostimulation treatments, with the Deltaproteobacteria, Firmicutes, Actinobacteria, Acidobacteria and Planctomycetes being inhibited and the Alpha- and Beta-proteobacteria and some unknown Gammaproteobacteria bacteria being enriched. In addition, different hydrocarbon-degraders came to power in the following turn. At the first stage, the Alcanivorax-veldXed Gammaproteobacteria bacteria dominated in the biostimulated soil and contributed mainly to the biodegradation of easily degradable portion of the heavy crude oil. Then the bacteria belonging to Alphaproteobacteria, followed by bacteria belonging to Candidate division OD1, became the dominant oil-degraders to degrade the remaining recalcitrant constituents of the heavy crude oil.     

Bacterial community composition and abundance in leachate of semi-aerobic and anaerobic landfills

Different municipal solid waste landfill methods and landfill ages had crucial impacts on bacterial abundance and composition in leachate.     

Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches

Combination of 16S rRNA gene clone library and cultivation for assessing of bacterial diversity of the microflora in bulking sludge was evaluated in the study.     

Comparison of bacterial community structures in two systems of a sewage treatment plant using PCR-DGGE analysis

Bacterial community was investigated during sludge bulking period using combination of PCR amplification of 16sRNA genes with DGGE analysis.