Inhibition mechanism of fermentation broth extract of Phomopsis sp. Strain S4 on cell membranes of Magnaporthe oryzae北大核心CSCD

The secondary metabolites of endophytic Phomopsis sp. strain S4 show antifungal activity against a variety of plant pathogens, which implies that strain S4 has potential prospect in crop disease control. The aim of this study was to explore the inhibition mechanisms of S4 against plant pathogenic fungi. Magnaporthe oryzae was used as the main pathogenic material for the research. Cell membrane changes were detected using antifungal experiments, scanning electron microscopy, Q RT-PCR, and cell content leaking experiments. The results of scanning electron microscopy showed that M. oryzae mycelia, after treatment with S4 fermented product extract, decreased in size, suggesting the integrity of the cell membrane was destroyed. The genes related to ergosterol synthesis, which plays an important role in membrane integrity, were studied though Q RT-PCR. The results showed that the expression levels of ERG1, ERG11, and ERG6 genes were down-regulated by 2.0, 1.40, and 2.7-fold, respectively, whereas that of ERG7 was up-regulated by 1.38-fold, which means the ergosterol synthesis pathway was destroyed. The physiological experiments also showed that the cell contents of M. oryzae mycelia treated with S4 fermented product extract leaked significantly, which was consistent with the Q RT-PCR results. The results showed that S4 fermentation broth exact could destroy the cell membrane integrity by inhibiting ergosterol synthesis, and eventually inhibit M. oryzae cell growth. © 2018 Science Press. All rights reserved.     

手机移动气象防灾减灾服务系统的设计与实现

应用目前先进的KJAVA技术，研究开发了江西省手机移动气象防灾减灾服务系统，简要介绍了该系统的基本原理、使用环境、信息流程、体系结构和主要功能。该系统投入业务运行以来，已得到各级党政领导和森林防火、防汛指挥、人工增雨等部门的好评，在防灾减灾方面取得了明显的社会经济效益。     

Influence of the Interaction of a 900 MHz Signal with Gender on EEG Energy: Experimental Study on the Influence of 900 MHz Radiation on EEG

Summary The effects of electromagnetic fields (EMF) emitted by cellular phones on human electroencephalogram (EEG) were studied during an auditory memory task. The experimental method and the experimental setup are introduced as a credible measurement method of EEG. 19 normal subjects (10 women and 9 men) performed the memory task both with and without exposure to a 900 MHz signal, emitted by a dipole antenna placed near the subjects' head. The energy of the EEG signals was calculated at the time domain. A Fourier transform of the EEG signals was done and the EEG energy was also calculated at the frequency domain. As the Parseval's theorem anticipates the energies were identical. The EEG energy was found concentrated at the four basic bands (α (8–13 Hz), β(14–30 Hz), δ (0–4 Hz) and θ(5–7 Hz)). The primary concern of the present study was the gender related influence of EMF on the spectral energy of EEG. The results show evidence of a strong gender—radiation interaction effect on the EEG energy and on the peak amplitudes within each of the four rhythms. Without radiation the spectral power of males is greater than of females, while under exposure the situation is reversed. Under the influence of EMF the spectral power of the males EEG is decreased while that of the females is increased. In conclusion both the baseline EEG and the changes effected to the EEG power spectrum under the influence of EMF seem to be gender dependent.     

Peroxidase activity,lignification and promotion of cell death in tobacco cells exposed to static magnetic field

Bio-effects of static magnetic fields on cell growth and cell death have been investigated in suspension-cultured tobacco cells as undifferentiated, embryonic plant cell model. The cells in their logarithmic growth phase were exposed to static magnetic field with the magnitudes of 10 mT and 30 mT for 5 h/day. Exposure to static magnetic field ceased the growth and caused an increase in cell death of exposed tobacco cells compared to those cells which were not treated with the field. Promotion of cell death was accompanied by a harmonized increase in the activity of peroxidase and increase of lignifcation of cell walls.     

我国废旧手机回收利用现状与对策分析

随着手机用户的增加，每年会产生大量的废旧手机。废旧手机一方面对环境构成潜在危害，一方面又具有回收利用的价值。本文针对我国目前废旧手机回收利用的现状，提出手机回收利用的措施，以有效应对电子垃圾污染，并实现资源的循环利用。     

手机电磁辐射安全评价

综述了手机辐射的原理、辐射危害、辐射场计算和辐射评价技术标准及其检测仪器,并提出了一些减小手机辐射的措施和建议.     

Cultured amniotic fluid cells for prenatal diagnosis of lysosomal storage disorders: A methodological study

The influence of culture conditions on the ultrastructure and enzyme activities of amniotic fluid cells are reported. Morphological changes were determined as a function of the number of lysosomal-like inclusion bodies per cell, and these results correlated to the activity of Thiexosaminidase, a-mannosidase, β-glucuronidase, arylsulphatase C and 5′ nucleotidase. The parameters examined were pH of the culture media, type of media, increasing cell passage and day of harvest. Our results indicate that enzyme activities are less sensitive to changes in culture conditions as compared to ultrastructural changes. We therefore recommend that in order to obtain reliable ultrastructural results for the diagnosis of storage disorders, cultures should be grown in MEM as the culture medium, the pH of the medium carefully monitored to remain below pH 7·4, examining the cultures no later than the eighth cell passage and no later than the 10th day after subculture.     

Impact of the composition of combustion generated fine particles on epithelial cell toxicity: influences of metals on metabolism

Inhaled airborne particulate matter (PM) represents a potentially significant health hazard to humans. Exposure to PM strongly correlates with pulmonary inflammation and incidences of severe respiratory distress, including increased hospital admissions for breathing disorders, asthma, emphysema, and chronic bronchitis. PM generated from the combustion of fuel oils and coals contain a number of water-soluble transition metals including Fe, V, and Zn.

We have evaluated the impact of PM types with varying composition collected from the combustion of oils and coals on the health and metabolism of lung cell cultures. Three colorimetric assays (sulforhodamine B (SRB), Janus green, and MTT) have been adapted to quantify the impact of PM on rat lung alveolar type II epithelial cells (RLE-6TN cells). The PM toxicity metrics evaluated were inhibition of cell proliferation (SRB and Janus green) and inhibition of cellular metabolism (MTT). Cell proliferation is inhibited in a consistent dose-dependent manner by PM concentrations from 25 to 250 μg/ml. At a level of 100 μg/ml, oil-derived PM diminishes cell metabolism by as much as 40% relative to controls; the degree of inhibition is strongly dependent on PM particle size and metal content. Conversely, coal-derived PM at the same dosage diminishes cell metabolism by no more than 20% relative to controls. All three assays provide highly repeatable results and consistent toxicity rankings of the PMs evaluated. Overall, metabolic inhibition as measured by the MTT assay was deemed the most appropriate metric for PM toxicity, primarily due to its applicability with in vivo-like confluent cell monolayers.     

Mobile phone use while riding a motorcycle and crashes among university students

Objective: Motorcycle crashes are a significant road safety challenge, particularly in many low- and middle-income countries where motorcycles represent the vast majority of their vehicle fleet. Though risky riding behaviors, such as speeding and riding under the influence of alcohol, have been identified as important contributors to motorcycle crashes, little is understood about the effect of using a mobile phone while riding on motorcycle crash involvement. This article investigates crash involvement among motorcycle riders with risky riding behaviors, particularly using a mobile phone while riding.

Methods: Data were obtained from an online survey of university students’ risky riding behaviors in Vietnam administered between March and May 2016 (n?=?665).

Results: Results show that 40% of motorcycle riders reported to have experienced a crash/fall and nearly 24% of motorcycle riders indicated that they had been injured in a crash/fall. Effects of mobile phone use while riding on safety of motorcycle riders are highlighted. Specifically, more frequent use of a mobile phone for texting or searching for information while riding is associated with a higher chance of being involved in a crash/fall. The results also show that drink riding is associated with a higher chance of being injured.

Conclusions: Overall this article reveals significant safety issues of using a mobile phone while riding a motorcycle, providing valuable insight for designing education and publicity campaigns.     

Self-regulation of driving speed among distracted drivers: An application of driver behavioral adaptation theory

Objective: The adaptive behavior of mobile phone–distracted drivers has been a topic of much discussion in the recent literature. Both simulator and naturalistic studies suggest that distracted drivers generally select lower driving speeds; however, speed adaptation is not observed among all drivers, and the mechanisms of speed selection are not well understood. The aim of this research was to apply a driver behavioral adaptation model to investigate the speed adaptation of mobile phone–distracted drivers.

Methods: The speed selection behavior of drivers was observed in 3 phone conditions including baseline (no conversation) and hands-free and handheld phone conversations in a high-fidelity driving simulator. Speed adaptation in each phone condition was modeled as a function of secondary task demand and self-reported personal/psychological characteristics with a system of seemingly unrelated equations (SURE) accounting for potential correlations due to repeated measures experiment design.

Results: Speed adaptation is similar between hands-free and handheld phone conditions, but the predictors of speed adaptation vary across the phone conditions. Though perceived workload of secondary task demand, self-efficacy, attitude toward safety, and driver demographics were significant predictors of speed adaptation in the handheld condition, drivers' familiarity with the hands-free interface, attitude toward safety, and sensation seeking were significant predictors in the hands-free condition. Drivers who reported more positive safety attitudes selected lower driving speeds while using phones.

Conclusion: This research confirmed that behavioral adaptation models are suitable for explaining speed adaptation of mobile phone distracted drivers, and future research could be focused on further theoretical refinement.