三峡水库175 m蓄水运行后鱼类汞污染风险研究

三峡水库的生态安全受到高度关注,三峡水库成库后是否会出现汞等重金属生态风险增加的“水库效应”,不仅是需要论证的科学命题,也是关系到库区人民身体健康的重要问题。2011年在三峡库区忠县至秭归段选择“湖沼化”特征最为显著的8条支流采集了鲤、鲢、鳙、鲇、鳜等常见经济鱼类标本,系统分析了库区鱼类标本肌肉中的总汞含量。研究结果表明,三峡库区支流鱼类肌肉中总汞含量最高为109.6 μg/kg,最低为30.5 μg/kg,平均含量为56.4 μg/kg。与蓄水前的调查结果相比,蓄水运用10 a后库区鱼类肌肉总汞水平尚未明显增加。但是,不同营养等级鱼类之间,肌肉总汞浓度水平随营养等级增加有明显增高,三峡水库水生食物链表现出显著的汞的生物累积。值得注意的是,底层鱼类汞的生物累积强度明显高于上层鱼类,暗示水库底栖过程的“汞活化”依然是需要关注的潜在生态风险     

Hemolysis,Fish Mortality,and LC-ESI-MS of Cultured Crude and Fractionated Golden Alga (Prymnesium parvum)1

Schug, Kevin A., Theodore R. Skingel, Sandra E. Spencer, Carlos A. Serrano, Cuong Q. Le, Christopher A. Schug, Theodore W. Valenti, Jr., Bryan W. Brooks, Laura D. Mydlarz, and James P. Grover, 2010. Hemolysis, Fish Mortality, and LC-ESI-MS of Cultured Crude and Fractionated Golden Alga (Prymnesium parvum). Journal of the American Water Resources Association (JAWRA) 46(1):33-44. DOI: 10.1111/j.1752-1688.2009.00389.x Abstract: Erythrocyte lysis and fish mortality assays, in combination with high performance liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analysis, were investigated for bioassay-guided fractionation of cultured golden alga (Prymnesium parvum). Intracellular constituents from isolated cell pellets and extracellular supernatant growth medium were fractionated by a variety of common separation modes, including reversed phase and normal phase solid phase extraction step fractionation procedures. For reversed phase fractionation of extracellular growth medium, one fraction was obtained that displayed hemolytic activity and adversely affected fish survival. Effective dose concentrations for this sample were similar in both assays and the LC-ESI-MS analysis of the fraction showed a number of mass spectral signals which were distinct to this fraction. Fractions obtained from separation of an ethanol extract of the lyophilized cell pellet provided one sample that was highly hemolytic, but not toxic to fish. Discrepancies such as this, along with notable fish behavioral responses from other nonhemolytic cell pellet fractions, problems with the use of unbonded silica gel for fractionation, and misleading mass spectral signatures are interesting in the context of our current understanding of P. parvum toxicity and remain to be investigated further. This work provides an account of ongoing research aimed toward comprehensive elucidation of toxic constituents produced by golden alga for the purpose of providing a better understanding and means to potentially remediate the ecological impact of this harmful bloom organism.     

长江口中华鲟自然保护区及临近水域鱼类种类组成现状

为了解长江口中华鲟自然保护区及其临近水域鱼类种类组成的现状,2004~2008年利用底拖网、插网、定置网和刺网等多种网具对该水域的鱼类组成状况进行了较为系统的调查。共调查到鱼类105种,隶属于18目43科86属,鱼类种类组成以鲈形目和鲤形目为主;生态类型中海洋鱼类和淡水鱼类各37种,河口定居性鱼类25种,洄游性鱼类6种;鱼类种类组成处于长江下游至东海的过渡类型,鱼类食性以底栖生物、有机碎屑和浮游动物食性为主,凶猛性鱼类较少。与以往调查资料相比,调查水域鱼类物种数有所减少,一些重要的物种更是濒临绝迹。保护区水域鱼类不仅具有较高的物种多样性、河口代表性、稀有性和很高的科研价值,同时其生态系统也较为脆弱,并已受到严重威胁,保护区的保护工作显得尤为重要.     

Residues of chlorpyrifos‐methyl in balsam fir foliage,forest litter,soil, stream water,sediment and fish tissue after double aerial applications of Reldan®

Abstract

Chlorpyrifos‐methyl was applied twice at 70 g A.I./ha by means of a fixed‐wing aircraft to a mixed coniferous forest near Allardville, New Brunswick. Residue in balsam fir foliage was highest (1 ppm wet wt) 1 hr after spraying and rapidly declined to about 30% within 1 day, but persisted at a very low level (0.03 ppm wet wt) for 125 days. Current year's foliage contained a higher level of residue than old foliage. Chlorpyrifos‐methyl persisted longer in forest litter than in soil. After 125 days, trace amounts (< 6 ppb wet wt) were still found in litter but were not detected in soil. In stream water the residue dissipated very rapidly; more than 90% disappeared 3 hours after the second application and were not detected after 4 days. Low‐level residue (< 0.1 ppm wet wt) was present in the sediment and persisted for 10 days. Although brook trout and slimy sculpin captured in the stream within 3 days of the second application contained residues (< 0.05 ppm fresh wt) none were detected in any fish captured, 9 and 47 days later.     

Nutrient release from fish silage using microwave-enhanced advanced oxidation process

The microwave-enhanced advanced oxidation process was used to treat fish silage for nutrient release and solids reduction prior to its use as a fertilizer for greenhouse operations. Fifteen sets of experiments with varying hydrogen peroxide dosages and treatment temperatures were conducted to evaluate the effectiveness of the process on the solubilization of fertilizer constituents. It was found that up to 26% of total Kjeldahl nitrogen could be released as ammonia with 6% hydrogen peroxide dosage at 170°C. An increase of nitrate/nitrite concentration was observed with higher hydrogen peroxide dosage and higher microwave temperature; the highest concentration of 10.2 mg L^{? 1} nitrates/nitrites was achieved at at 170°C and 6% H₂O₂ dosage. Up to 20 ± 9.5% of total chemical oxygen demand was reduced at temperatures between 120 and 170°C. Large quantities of volatile fatty acids were generated at lower temperatures, corresponding to an increase in soluble chemical oxygen demand, but not at higher temperatures. The treatment of fish silage using the microwave-enhanced advanced oxidation process appears to be promising.     

Nationwide monitoring of mercury in wild and farmed fish from fresh and coastal waters of Korea

Mercury (Hg) concentrations were monitored in wild and cultured fish collected from fresh and coastal waters in the Korean peninsula from April 2006 to August 2008 nationwide. Total Hg concentrations were reported for 5043 fish samples, including 78 species from 133 locations. Significant interspecies variation was noted in the Hg levels. The average Hg concentration in each fish species ranged from 6.31 μg kg⁻¹ for mullet (Mugil cephalus) to 200 μg kg⁻¹ for mandarin fish (Siniperca scherzeri). Among the species collected, the maximum concentration of Hg, 1720 μg kg⁻¹, was measured in an Amur catfish (Silurus asotus). Only wild freshwater fish exceeded the WHO ingestion standard. Wild freshwater piscivorous fish samples from a large artificial upstream lake contained the highest Hg levels. Hg concentrations were compared between fish groups categorized as wild and farmed fish from freshwater and coastal waters. Although the wild freshwater fish had similar size ranges, their Hg concentrations were higher than those of the other groups. Compared to the feed of farmed marine and freshwater fishes, the prey of wild freshwater fish had a higher Hg concentration, and the total Hg concentrations in freshwater and associated sediment samples were higher than those in coastal water and associated sediment samples. In the freshwater environment, piscivorous fish bioaccumulated two times more Hg than carnivorous and omnivorous fish and four times more than planktivorous fish. The difference in Hg concentrations among trophic groups might have been due to differences in the size of fish, in addition to the variations among different trophic groups. These data will be useful for developing the fish consumption advisory as a management measure to reduce Hg exposure.     

Hexabromocyclododecanes (HBCDs) in marine fishes along the Chinese coastline

This study reports concentrations of hexabromocyclododecanes (HBCDs) in two species of marine fish, large yellow croaker (Pseudosciaenacrocea) and silver pomfret (Pampusargenteus) (n = 46), from nine Chinese coastal cities (Dalian, Tianjin, Qingdao, Shanghai, Zhoushan, Wenzhou, Fuzhou, Quanzhou and Xiamen). HBCDs were detectable in all samples analyzed, indicating ubiquitous contamination of these compounds in the Chinese coastal environment. The average total HBCD concentration was 3.7 ng g⁻¹ lipid weight (range: 0.57-10.1 ng g⁻¹ lipid weight), which is relatively lower than other regions of the world, especially Europe, where HBCDs are intensively used. Among the three individual HBCD isomers (α-, β- and γ-HBCD) in all fish samples, the α-isomer showed a remarkable predominance (from 87.5% to 100% of total contribution), indicating its higher bioaccumulative potential. Geographically, the highest HBCD level present in fish was found in Dalian in northern China, and the lowest occurred in Wenzhou. Estimated daily intakes of HBCDs via fish consumption for the Chinese population were 0.004-1.00 ng kg body weight⁻¹ d⁻¹. These exposure levels were much lower than the effect levels.     

Inputs of antifouling paint-derived dichlorodiphenyltrichloroethanes (DDTs) to a typical mariculture zone (South China): potential impact on aquafarming environment

Existing evidence indicated that dichlorodiphenyltrichloroethane (DDT)-containing antifouling paints were an important source of DDT residues to mariculture zones. However, the magnitude of the impact on aquafarming environment has remained largely unknown. In the present study, the concentrations of DDT and its metabolites (designated as DDXs) were determined in harbor sediment and antifouling paint samples collected from a typical mariculture zone in South China. Compositional and concentration correlation analyses implicated the DDT-containing antifouling paints for fishing boat maintenance as an important source of DDT in the mariculture zone. The annual emission of DDXs to the study region was estimated at 0.58 tons/yr. Furthermore, a comparison of the expected DDT loadings in pelagic fish and field measurements indicated that fish feed especially trash fish was a major source of DDTs in the fish body. Nevertheless, the use of DDT-containing antifouling paints should be limited to prevent further deterioration in aquafarming environment.     

Distributions of polyhalogenated compounds in Hudson River (New York, USA) fish in relation to human uses along the river

PCBs (as Aroclor concentrations) have been extensively examined in fish along the Hudson River, but other xenobiotic chemicals in fish have had limited assessment. This study determined concentrations and congener distributions of polybrominated diphenyl ethers (PBDEs), polybrominated and polychlorinated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs and PCDD/Fs), and polychlorinated biphenyls (PCBs) in smallmouth bass and striped bass taken from a 385 km reach of the Hudson River. Concentrations of PBDEs and PCBs in smallmouth bass, and PCBs in striped bass, were positively related to human uses of the compounds in the basin. Generally low levels of PCDD/Fs were found. One striped bass, however, contained elevated 2,3,7,8-TCDD, indicating exposure to a known source in the adjacent Newark Bay-Passaic River basin. PBDDs were generally below detection. PBDFs were present in four of 18 smallmouth bass, but were not detected in striped bass. Dioxin-like PCBs contribute most to 2,3,7,8-TCDD toxic equivalents in 29 of 30 samples.     

Design-based estimators and power analyses of trend tests for the proportion of fish that exhibit gross pathological disorders

Methods for estimating the proportion of fish that exhibit gross pathological disorders and for estimating the variance of these estimates are defined. The methods are for the situation in which a probability-based sampling design is used to collect fish for examination, but geographic locations (rather than individual fish) are assigned probabilities of being selected for sampling. To illustrate the use of the methods, they are applied to data collected during the 1992 EMAP- Estuaries sampling program in the Louisianian Province (i.e., the Gulf of Mexico). Separate estimates of the proportion of fish with gross pathological disorders are computed for demersal species, commercial species, pelagic species, and all species as one group. In addition, a test for trend in the proportion of fish that exhibit gross pathological disorders is defined, and analyses of the power of the test are presented. The power analyses are based on a general underlying model of the random distribution patterns of fish and the random process of catching fish. The power analyses also take into account the features of the sampling designs used for collecting fish. Component parameter estimates were computed using data from the 1992 EMAP-Estuaries sampling program in the Louisianian Province. Results from these analyses suggest that the EMAP-Estuaries sampling designs are capable of detecting a 0.15% change per year in the proportion of fish (all species groups combined) with gross pathological disorders in estuaries of the Louisianian Province over a 12-year period with a power of at least 80%. © Rapid Science 1998