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81.
82.
Jiaen Liu Dr Willy Lissens Paul Devroey Andre Van Steirteghem Ingeborg Liebaers 《黑龙江环境通报》1993,13(9):873-880
The efficiency of the polymerase chain reaction (PCR) in detecting the cystic fibrosis (CF) ΔF508 mutation (which is the most common mutation of CF) was assessed in single human blastomeres. Twenty-one human immature oocytes (germinal-vesicle-stage oocytes) that had been donated for research were matured in vitro and a single spermatozoon from a carrier of the CF ΔF508 mutation was injected into the ooplasm. Fourteen embryos were obtained after intracytoplasmic sperm injection (ICSI). PCR analysis was carried out on 70 single blastomeres isolated from these 14 embryos. The results showed that the efficiency of DNA amplification by PCR in single nucleate blastomeres was 94 per cent (59/63). There were no false-positive results since none of the blank samples or the blastomeres without a nucleus showed an amplified signal. We found that nine embryos were homozygous for the unaffected genotype and that four embryos were heterozygous since they contained both the unaffected and the ΔF508 genotype. In a four-cell embryo, we observed the homozygous unaffected genotype in one blastomere and a heterozygous ΔF508/unaffected genotype in the other three blastomeres. 相似文献
83.
流动注射荧光猝灭法测定环境水样中痕量磷 总被引:6,自引:0,他引:6
在硫酸溶液中,P(Ⅴ)与钼酸盐、钒酸盐生成磷钼钒杂多酸,使罗丹明6G荧光猝灭,据此建立了测定痕量磷的流动注射荧光猝灭方法。方法的测定范围为2~90μg/L,进样频率为35样/h。采用本法测定环境水样中痕量磷,结果令人满意。 相似文献
84.
废塑料在炼铁工艺中的应用 总被引:1,自引:0,他引:1
介绍了废塑料的处理和利用方法 ,叙述了高炉喷吹废塑料的工艺、优点和国外的实际应用。通过比较 ,认为在高炉中应用废塑料作还原剂能带来较好的社会效益和经济效益 ,指出随着喷吹技术的不断完善 ,高炉喷吹废塑料在我国具有广阔的应用前景 相似文献
85.
Paul F. Hudak 《环境质量管理》2019,28(4):63-66
Efficient monitoring systems addressing the difficulty of detecting narrow contaminant plumes originating from unknown point sources are needed for modern landfills. A low‐discharge extraction and accompanying injection wells could potentially address this problem. This hypothetical computer‐modeling study involved a three‐well detection system consisting of one extraction and two injection wells at a rectangular landfill in a shallow, unconfined aquifer. The extraction and injection wells were located near the landfill's downgradient and cross‐gradient corners, respectively. Each injection well pumped at half the rate of the extraction well. A minimum pumping rate of 1.1 cubic meters per day was determined for the three‐well system; at this rate, all contaminant plumes originating within the landfill's footprint entered the extraction well prior to reaching a downgradient property boundary. In comparison, five passive (not pumped) wells detected all contaminant releases from the landfill. Results of this study suggest that a low‐discharge extraction well with accompanying injection wells may be an effective contaminant detection strategy at some waste impoundments. 相似文献
86.
环保注水--油田污水治理的新途径 总被引:3,自引:0,他引:3
介绍了油田污水排放现状、特征及处理难点,定义了环保注水新概念。重点介绍了环保注水的特点、工艺流程、现场试验.探讨了油田污水治理、保护环境的新途径。 相似文献
87.
88.
Coralie Chasset Jerker Jarsjö Mikael Erlström Vladimir Cvetkovic Georgia Destouni 《International Journal of Greenhouse Gas Control》2011,5(5):1303-1318
Deep saline aquifers have large capacity for geological CO2 storage, but are generally not as well characterized as petroleum reservoirs. We here aim at quantifying effects of uncertain hydraulic parameters and uncertain stratigraphy on CO2 injectivity and migration, and provide a first feasibility study of pilot-scale CO2 injection into a multilayered saline aquifer system in southwest Scania, Sweden. Four main scenarios are developed, corresponding to different possible interpretations of available site data. Simulation results show that, on the one hand, stratigraphic uncertainty (presence/absence of a thin mudstone/claystone layer above the target storage formation) leads to large differences in predicted CO2 storage in the target formation at the end of the test (ranging between 11% and 98% of injected CO2 remaining), whereas other parameter uncertainty (in formation and cap rock permeabilities) has small impact. On the other hand, the latter has large impact on predicted injectivity, on which stratigraphic uncertainty has small impact. Salt precipitation at the border of the target storage formation affects CO2 injectivity for all considered scenarios and injection rates. At low injection rates, salt is deposited also within the formation, considerably reducing its availability for CO2 storage. 相似文献
89.
TOMIOKA Kenichi OGUMA Koichi SEKITatsuya 《环境化学》2003,22(3):211-214
1 IntroductionLeadiswellknownasoneofthemosttoxicmetalsandnotedinthefieldofenvironmentalchemistry[1 ] .Theleadconcentrationinnaturalwaterisextremelylow (sub μg·l-1 level)sothatsomepreconcentrationstepmustbeincludedtoassurethesensitivityofaconventionalin… 相似文献
90.