Does improved waste treatment have demonstrable biological benefits?

Since 1972, 10 benthic surveys and 9 static fish bioassays have been conducted to assess the impact of AVTEX Fibers, Inc. effluent on the lower South Fork of the Shenandoah River. AVTEX (formerly FMC Corp.) is a rayon and polyester fibers plant located in Front Royal, Virginia. Benthic samples were taken at four stations, one above and three below the plant discharges. Single surveys in 1972 and 1973 indicated a severe impact on the benthic community along the right side of the river, below the plant, as a result of the channelized effluent. Diversity values (¯d) were low (0–2.42) and numbers of taxa and organisms were reduced. A fish bioassay in 1973 indicated the effluent to be acutely toxic at the 34.5% level (mixture of effluent and river water). In early 1974, FMC Corp. constructed an activated sludge treatment system to reduce BOD and supplement the neutralization and chemical precipitation (zinc hydroxide and liquid-solid separation) facilities that had been used to treat waste waters since 1948. After the new equipment was placed in operation, the previously stressed area became more stable. In 1975 and 1976 the stressed area exhibited greater ¯d values (1.19–3.39) and an increased number of taxa and organisms. Bioassays showed the effluent to be acutely toxic to fish only once since 1973. The major improvements in the effluent were a 70% reduction in BOD₅ and a 60% reduction in the amount of zinc entering the river. Community conditions in 1977 indicated a partial remission of improvement, probably due to drought conditions.The rehabilitation of damaged ecosystems is a process important to all biologists. An important factor in encouraging industry to participate in this activity is evidence that improved waste treatment will often have demonstrable biological benefits rather soon. As data accumulate on the recovery process it may be possible to predict the degree of rehabilitation and time required more precisely.     

Risk assessment for selected xenobiotics by bioassay methods with higher plants

Different bioassays with higher plants were approved for use in a bioassay procedure for testing of xenobiotics according to the German Chemicals Act. Selected environmental pollutants (atrazine, cadmium chloride, 2,6-dichlorobenzonitrile, pentachlorophenol, potassium dichromate, thiourea), all from a list of reference chemicals, were tested with these methods. Dose-response curves for growth of oats and turnips were evaluated in soil and vermiculite (nonsorptive substrate), and availability to plants was calculated by comparing the EC₅₀ values for one chemical in both substrates. The most active chemical was atrazine, followed by 2,6-dichlorobenzonitrile, pentachlorophenol, potassium dichromate, cadmium chloride, and thiourea. The least available compound to plants was pentachlorophenol, tested with turnips (Brassica rapa var.rapa). The strongest inhibition of germination, demonstrated in an in vitro assay with garden cress (Lepidium sativum), was found with 2,6-dichlorobenzonitrile, the lowest with atrazine. The effect of an extended exposure of the plants to the chemicals was evaluated in a long-term bioassay with oats (Avena sativa) in hydroponic culture. Several dose-response curves during the growing period were derived. It was found that the EC₅₀ values for atrazine and thiourea decreased markedly during the first four weeks; thereafter the changes were much smaller. As an overall conclusion, a bioassay procedure is proposed that can be included in the graduated plan recommended by the German Chemicals Act.     

Progress in herbicide determination with the thylakoid bioassay

Chloroplast thylakoids are used as biological units to determine herbicides in different kinds of water samples as well as in aqueous extracts of compost, soil or food samples. The thylakoid bioassy shows clearly inhibition of fluorescence yield in the presence of photosystem II specific herbicides. Due to this method the ecotoxicological effect of samples with unknown pollutants can be tested fast and cost effective. It has been proven that all photosynthetic active compounds are recorded at the same time because only additive interactions occur. Therefore, the contamination level can be expressed as cumulative parameter for photosystem II active substances. Application was improved clearly by the addition of the radical scavenger sodium ascorbate to the isolation media and by a higher concentration of the measuring medium. A new data evaluation method is described yielding in a lower detection limit of 0.4 μg diuron/1. The guidelines for the quality of water for human consumption with an allowable concentration of pesticides in groups is 0,5 μg/1 [1,2] and can be controlled with the thylakoid bioassay without performing any preconcentration steps.     

Fractionation and Biotoxicity of Heavy Metals in Urban Dusts Collected from Hong Kong and London

Samples of urban dusts, road site dusts and car park dusts, were collected at two selected sites each in Hong Kong and London. Sequential extraction was used to characterise the chemical compositions of these urban dusts. Copper, lead, zinc, pH, electrical conductivity and organic content were measured. Biotoxicity tests of urban dusts were conducted on higher plants (Brassica chinensis and Lolium perenne), a dinoflagellate green alga (Dunaliella tertiolecta) and luminescent bacteria (Photobacterium phosphoreum). A significant correlation was found between total lead (r =; – 0.70, p < 0.01) and zinc (r =; – 0.74, p < 0.05), and the 20 min – EC50 using P. phosphoreum. In addition, there was a significant correlation (r = – 0.72, p < 0.01) between the exchangeable lead content in dust and the 48 h- EC30 using D. tertiolecta. No specific trend was obtained for higher plants. Total lead and zinc contents were higher in dusts from London while the percentage of exchangeable fraction of metal contents was higher in those from Hong Kong.     

Persistence of metsulfuron‐methyl in two soils

Abstract

The persistence of metsulfuron‐methyl in sandy loam and clay soil incubated at different temperatures and moistures contents was investigated under laboratory conditions using longbean (Vigna sesquipedalis L.) as bioassay species. A significant degradation of metsulfuron‐methyl was observed in non‐autoclaved soil rather than the autoclaved soil sample. At higher temperature, the degradation rate in non‐autoclaved soil improved with increasing soil moisture content. In non‐autoclaved sandy loam and clay soil, the half‐life was reduced from 9.0 to 5.7 and from 11.2 to 4.6 days, respectively when moisture level of sandy loam increased from 20 to 80% field capacity at 35°C. In the autoclaved soil, herbicide residue seems to have been broken down by non‐biological process. The rate of dissipation was slightly increased after the second application of the herbicide to non‐autoclaved soils but not in autoclaved soil, indicating the importance of microorganisms in the breakdown process.     

Assessment of pollution in road runoff using a Bufo viridis biological assay

Road runoff is a major source of environmental pollution, significantly threatening nearby aquatic habitats. Chemical analyses indicate high pollutant concentrations in the road’s “first flush”, but bioassays are more advantageous for addressing the cumulative effects of the numerous pollutants within the runoff. We used Bufo viridis embryos and larvae to assess the toxicity of road runoff from two major highways in Israel. We show, for the first time, that exposure to midseason runoff not only has an adverse effect on growth and development rates of B. viridis larvae but can also lead to increased rates of morphological deformations. Seasonal first flushes, despite having higher metal concentrations, did not adversely affect the toad larvae, apparently due to a counter effect of organic matter that potentially served as a supplementary energy resource. Road runoff can be a major cause for a qualitative decrease in the quality of aquatic habitats threatening amphibians in Israel.     

黄浦江某支流河水生物毒性研究

用鱼类回避试验和微生物毒性检测等两种生物检测技术来判断黄浦江某支流的生物毒性，结果表明，该支流河水能引起鱼类强烈回避，毒性显著，河水污染以有机物为主，河水中的１．２￣１．４μｍ悬浮颗粒物上吸附有大量的微生物抑制物，在碱性条件下，这些物质有释放趋势并产生生物毒性，可抑制微生物群落的功能，而酸性提取物不显微生物毒性。     

Insecticides biomarker responses on a freshwater fish Corydoras paleatus (Pisces: Callichthyidae)

This study was undertaken to investigate the effects of sublethal concentration of three different classes of insecticides (carbamate, organophosphate, and pyrethroid compounds) on the freshwater fish Corydoras paleatus. For this purpose, fish were exposed for 96 hours to commercial pesticides. Different biomarkers were analyzed as levels of lipid peroxidation (LPO), piscine micronucleus test, and enzymatic activities of catalase (CAT), glutathione S-transferase (GST), and acetylcholinesterase (AChE). The brain AChE was inhibited with carbaryl and methyl parathion, but no inhibition was observed with deltamethrin. The insecticides did not cause oxidative stress or genotoxic effects at the tested concentrations. Further studies are needed to elucidate the biotransformation of Corydoras paleatus insecticides and a possible resistance mechanism.     

Phytotoxicity and persistence of metribuzin residues in black soil

A bioassay procedure based on the root and shoot growth of cucumber and sorghum were used to study the phytotoxicity, as affected by varying the concentration of metribuzin, and persistence of metribuzin residues in soil. Sorghum and cucumber were found to be very susceptible test plant species to metribuzin. It was found that the persistence and phytotoxicity increased with increasing rate of application of metribuzin, and the relationship between herbicide concentration and phytotoxicity was best described by a quadratic equation. Sorghum and cucumber plants emerged as very sensitive new bioassay plants for metribuzin and could detect residues even at 0.010 and 0.046?ppm in the post harvest soil of potato crop.     

摇蚊幼虫的水生态毒理学研究进展

本文综述了国外对摇蚊幼虫的水生态毒理学研究的最新进展，包括生物检测、生物积累和遗传毒理研究等几方面．国外的研究表明摇蚊幼虫可以作为常用试验生物（鱼类和蚤类）的补充，用于有毒化学品的急、慢性毒性生物检测和遗传毒性研究，以及生物积累试验，以评价化学品的生态效应，并对污染物进行生物监测．