盐生杜氏藻烯醇酶基因启动子的克隆分析及胁迫转录应答

为了解盐生杜氏藻(Dunaliella salina)烯醇酶(Enolase)在渗透耐受中的具体功能,利用基因组步行方法和巢式PCR,从D.salina中克隆了烯醇酶基因DsENO 5’上游约2 000 bp的调控序列,并对其进行序列分析.分析表明,它包含多个与转录调控有关的保守序列(如CAAT-box,TATA-box),富含光﹑干旱及其它胁迫应答元件.利用实时荧光定量PCR的方法,研究了高渗﹑高温以及低温外界胁迫条件下DsENO的转录情况,发现其受高渗强烈抑制,高温显著诱导而低温微弱诱导.     

秀丽线虫适应响应的形成与调控机理

除了环境暴露评价,在环境毒理学研究中模式动物秀丽线虫也已经被应用于适应响应形成及其调控机理研究.在综述中,系统介绍了秀丽线虫中存在的几种形式毒物诱发的适应响应,即同一因素适应响应、交叉适应响应与特殊形式的适应响应;讨论了目前已经揭示出的6种影响适应响应形成因素;分析了适应响应形成可能机制,即抗氧化应激体系、热激蛋白、金...     

大跨径钢管混凝土拱桥减震控制装置参数的研究

大跨度桥梁结构的减震控制研究对于桥梁结构的抗震安全具有重要意义。本文以主跨368m的茅草街大桥为研究对象,基于ANSYS建立了该桥的三维有限元模型,并采用子空间迭代法分析了该桥的动力特性。在此基础上进行了大跨度钢管混凝土拱桥的地震响应及减震控制研究,重点进行了弹性连接装置和粘滞阻尼器减震效果的参数敏感性分析,并对比分析了不同位置布设减震装置时的效果。结果表明,纵飘振型对该桥肋纵向相对位移的贡献最大;弹性连接装置和阻尼器均能有效减小地震作用下该桥的肋梁纵向相对位移;综合考虑各关键部位的地震响应时,同时采用两类减震装置并将其分散布置时的减震效果最佳。结论可供大跨度中承式钢管混凝土系杆拱桥的抗震设计参考。     

某大型复杂结构地震响应与控制研究

针对某大型复杂结构的地震响应及控制策略进行了研究.首先,建立该结构的整体有限元模型,采用Ritz向量法对结构进行模态分析,研究该复杂结构的振动特性,基于位能加权平均法求解了该复杂结构等效阻尼比;随后,研究了不同布置型式下粘滞消能支撑的受力特点和计算模型;最后,对该结构进行减振设计,分析了不同消能支撑布置形式下的减振效果...     

中国石油数字化应急预案系统研究

中国石油高度重视应急预案体系建设工作,集团公司和所属企业均在风险分析的基础上,建立了“横向到边、纵向到底”的应急预案体系,为有效应对各类突发事件提供了重要的支持.旨在对文本应急预案的数字化应用技术进行研究,以进一步提高应急预案的可操作性.首先,探讨了数字化应急预案的概念,分析了国内外数字化应急预案系统技术的研究现状.其次,基于中国石油数字化应急预案系统的需求分析,从业务流程设计、系统部署设计和系统功能设计等三个方面对其进行了研究和分析.最后,立足于中国石油应急信息系统建设实际,提出了中国石油建设数字化预案系统建设的实施步骤.     

Modeling Nutrient Export From Coastal California Watersheds

Nitrate and phosphate export coefficient models were developed for coastal watersheds along the Santa Barbara Channel in central California. One approach was based on measurements of nutrient fluxes in streams from specific land use classes and included a watershed response function that scaled export up or down depending on antecedent moisture conditions. The second approach for nutrient export coefficient modeling used anthropogenic nutrient loading for land use classes and atmospheric nutrient deposition to model export. In an application of the first approach to one watershed, the nitrate and phosphate models were within 20% of measured values for most storms. When applied to another year, both nitrate and phosphate models generally performed adequately with annual, storm‐flow, and base‐flow values within 20% of measured nutrient loadings. Less satisfactory results were found when applied to neighboring watersheds with difference percentages of land use and hydrologic conditions. Application of the second approach was less successful than the first approach.     

DROUGHT POLICY IN THE U.S. AND AUSTRALIA: A COMPARATIVE ANALYSIS1

ABSTRACT: Federal and state governments in the United States and Australia have come to play a key role in attempts to mitigate the impact of drought. Government actions have usually taken the form of loans and grants to individual citizens, businesses and municipalities experiencing the hardship of drought. Most of these actions have occurred in an environment of crisis management, rather than as a result of clearly stated policy objectives. Based on a review and evaluation of recent drought policy in the United States and Australia, recommendations are offered on ways to improve the United States’approach. A national drought plan is suggested as an efficient mechanism through which these recommendations could be implemented. States should also become more actively involved in drought assessment and response, but these actions must be coordinated with federal actions.     

基于GIS的环境应急指挥系统的研究

叙述了环境突发事件应急处理工作程序和流程,环境应急指挥基于GIS系统下各模块的功能作用。应急指挥系统的应用对减少突发环境污染事故的发生以及事故造成的社会、环境危害,提高中国应对突发事故的能力具有重要的社会效益。     

A proteomic analysis of rice seedlings responding to 1,2,4-trichlorobenzene stress

The proteomic analysis of rice （Oryza sativa L.） roots and leaves responding tol,2,4-trichlorobenzene （TCB） stress was carded out by two dimensional gel electrophoresis, mass spectrometric （MS）, and protein database analysis. The results showed that 5 mg/L TCB stress had a significant effect on global proteome in rice roots and leaves. The analysis of the category and function of TCB stress inducible proteins showed that different kinds of responses were produced in rice roots and leaves, when rice seedlings were exposed to 5 mg/L TCB stress. Most responses are essential for rice defending the damage of TCB stress. These responses include detoxication of toxic substances, expression of pathogenesis-related proteins, synthesis of cell wall substances and secondary compounds, regulation of protein and amino acid metabolism, activation of methionine salvage pathway, and also include osmotic regulation and phytohormone metabolism. Comparing the TCB stress inducible proteins between the two cultivars, the β-glucosidase and pathogenesis-related protein family 10 proteins were particularly induced by TCB stress in the roots of rice cultivar （Oryza sativa L.） Aizaizhan, and the glutathione S-transferase and aci-reductone dioxygenase 4 were induced in the roots of rice cultivar Shanyou 63. This may be one of the important mechanisms for Shanyou 63 having higher tolerance to TCB stress than Aizaizhan.     

湿地退耕还湿与替代生计选择的农民响应研究——以三江自然保护区为例

通过对三江自然保护区核心区内6个村屯和实验区内5个村屯的310家农户家庭就退耕还湿和替代生计选择的问卷调查分析发现,在退耕还湿问题上,不同区域位置、年龄结构和耕地拥有量决定了他们对退耕还湿支持意愿的不同,表现在实验区内的农民和农民中的年轻人支持率要高,耕地在6~12hm²之间的农民支持率最低;农民反对退耕还湿的原因和农民接受退耕还湿的补偿选择都凝结着农民对现有耕地及补偿制度的思考。在替代生计选择上,当地居民没有明确的目标,多倾向于有保障的种植业调整。研究表明:农民对退耕还湿的支持主要取决于能否保证自身的经济利益,并且与农民的收入来源和谋生能力有关;替代生计发展的不健全和不稳定,使得农民仍把土地作为重要的生活保障。缺乏明确的退耕还湿补偿制度和替代生计引导是退耕还湿实施的制度障碍和政策缺失;基于当地农民考虑,提出了核心区生态移民、缓冲区传统农业改造和实验区多元化产业发展总体替代生计模式。