限制性片段长度多态性分析(ARDRA)方法对重金属污染土壤中细菌群落多样性的研究

限制性片段长度多态性分析(ARDRA)是用限制性内切酶消化扩增后的细菌16S rDNA,通过凝胶电泳分析微生物种群多样性的分子标记技术,该技术在微生物生态学领域有着广泛的应用.实验采用2种途径评价了这种方法应用于研究土壤中细菌种群多样性的可行性.通过对未污染土壤细菌16S rDNA的克隆,获得了经内切酶HaeⅢ消化的不同16S rDNA的ARDRA类型,并对测序的19个克隆子建立了系统发育树.此外,还对不同Cd和Pb污染水平的土壤微生物进行了群落水平上的ARDRA分析.结果表明,5 mg·kg-1 Cd和500 mg·kg-1 Pb污染对微生物群落组成有一定影响,但此范围内的重金属含量对微生物群落多样性没有相关性影响.     

不同生态下云南稻核心种质孕穗期耐冷性状的表型多样性差异

以504份云南地方稻核心种质为材料,在昆明自然低温平均18℃(冷害)和新平27℃(正常)条件下进行孕穗期耐冷性状的表犁多样性生态差异分析.结果表明:(1)在3种生态环境下,云南稻核心种质11个耐冷性状的平均值、标准差、变异系数等参数存在一定差异,总趋势呈现籼稻(Indica)不同环境间这3个特征值差异较粳稻(Japonica)明显;弱耐冷系统群差异较强耐冷系统群明显.(2)不同环境间11个耐冷性状Shannon-weaver遗传多样性指数存在明显差异,体现为正常环境高于冷害环境,粳稻(Japonica)高于籼稻(Indica),强耐冷材料高于弱耐冷材料.这种差异主要由剑叶长、穗颈长、穗下节长、1～2节长、穗长、主穗实粒数和结实率等7个耐冷性状差异所致.     

鄱阳湖水体细菌群落组成及遗传多样性

利用建立细菌16S rRNA基因文库和限制性内切酶长度多态性(RFLP)分析的方法,对鄱阳湖南、北湖区水体的细菌多样性进行了研究. 结果表明,鄱阳湖水体细菌多样性较高,且南湖区2007年水体的细菌多样性明显高于其2006年水体和北湖区水体. 主要细菌组成分析表明,鄱阳湖水体中的349个阳性克隆代表的167种基因型分别属于十大细菌类群,变形菌门(Proteobacteria)在4个克隆文库中占据了47%～81%,数量极其丰富,尤其是亚型的β-变形菌为优势菌群; 拟杆菌门(Bacteroidetes)和放线菌门(Actinobacteria)也广泛分布,且有2.9%～17.1%的克隆数属于未分类细菌. 研究结果进一步提示鄱阳湖水体有一定程度的富营养化.      

蚯蚓/铈锰改性生物炭对As污染红壤中细菌多样性和群落结构的影响

在明确铈锰改性生物炭(MBC)和赤子爱胜蚓(Eisenia foetida)联合修复可有效固定红壤As的前提下,采用高通量测序技术,探讨MBC与赤子爱胜蚓联合作用对As污染红壤中细菌多样性和群落结构的影响.结果表明,蚯蚓与生物炭/MBC单一和联合处理中,细菌多样性指数均以单一蚯蚓处理下最高,而单一生物炭、MBC的施用均...     

A Critical Review of Assumptions About the Prairie Dog as a Keystone Species

Cynomys spp.) have been labeled as keystone species because of their influence on biological diversity and ecosystem function. However, the validity of several assumptions used to support keystone status is questionable. We review the strength of the evidence and the magnitude of the prairie dog's effects on ecosystem structure and function. We use this review to reevaluate the keystone role for prairie dogs. Our goal is to encourage sound management of the prairie dog ecosystem by improving the ecological foundation of their keystone status. Our review confirms that prairie dogs affect a number of ecosystem-level functions but that their influence on prairie vertebrates may be less than previously suggested. Species richness and abundance patterns were variable among plants, mammals, and birds and were not consistently higher on prairie dog colonies compared to uncolonized areas. In addition, only nine of the 208 species listed in the literature as observed on or near prairie dogs colonies had quantitative evidence of dependence on prairie dogs. Abundance data indicated opportunistic use of colonies for an additional 20 species. A total of 117 species may have some relationship with prairie dogs, but we lacked sufficient data to evaluate the strength of this relationship. The remaining 62 species may be accidental or alien to the system. Despite our conclusion that some prairie dog functions may be smaller than previously assumed, collectively these functions are quite large compared to other herbivores in the system. We suggest that prairie dogs also provide some unique functions not duplicated by any other species and that continued decline of prairie dogs may lead to a substantial erosion of biological diversity and landscape heterogeneity across prairie and shrub-steppe landscapes. Thus, we concur that keystone status for prairie dogs is appropriate and may aid conservation efforts that help protect species dependent on prairie dogs and support other important ecosystem functions.     

Spatial statistics for modeling of abundance and distribution of wildlife species in the Masai Mara ecosystem, Kenya

This study illustrates the use of modern statistical procedures for better wildlife management by addressing three key issues: determination of abundance, modeling of animal distributions and variability of diversity in space and time. Prior information in Markov Chain Monte Carlo (MCMC) methods is used to improve estimates of abundance. Measures of autocorrelation are included when modeling distributions of animal counts, and a diversity index to indicate species abundance and richness for large herbivores is developed. Data from the Masai Mara ecosystem in Kenya are used to develop and demonstrate these procedures. The new abundance estimates are up to 35% more accurate than those obtained by existing methods. Significant temporal changes in spatial patterns are found from a space-time analysis of elephant counts over a 20-year period, with strong interactions over 5 km and 6 months space and time separations, respectively. The new diversity index is sensitive to both high abundance and species richness and is also able to capture year to year variation. It indicates an overall marginal decrease in diversity for large herbivores in the Mara ecosystem. The space-time analyses and diversity index can easily be computed thereby providing tools for rapid decision making.     

Vegetation zonation and management in the Damietta estuary of the River Nile

The zonation of the vegetation along the saline and freshwater marshes of the Damietta estuary of the Nile River was studied from near the river mouth to 20 km upstream. Downstream, the estuarine water is almost stagnant and highly saline with high concentrations of nutrients. This makes the habitat unsuitable for euhydrophytes. Upstream, the vegetation consists mostly of freshwater macrophytes. 75 sampling plots were established in representative stands of the upshore and upstream vegetation zones. Classification and ordination of the data revealed seven vegetation types, indicated A—G. The dominant species of the saline marshes werePhragmites australis, Tamarix nilotica andArthrocnemum macrostachyum (A),Zygophyllum aegyptium andPolygonum equisetiforme (B),Cynodon dactylon andSuaeda vera (C). In the freshwater marshes the dominants were:Ludwigia stolonifera, Persicaria lapathifolia (D),Typha domingensis (E),Eichhornia crassipes (F) andCeratophyllum demersum (G). The first axis of the ordination axis obtained with Detrended Correspondence Analysis can be associated with the upstream gradient. It separates the salt marsh vegetation groups from those of the freshwater marshes. Plant species richness increased upshore along both saline and freshwater marshes. The concentration of dominance increased upstream. Some aspects of proper management of estuarine vegetation are mentioned.     

干旱地区土地利用结构变化与生态服务价值的关系研究——以酒泉市为例

利用酒泉市1997-2006年土地利用变更调查数据和相关统计资料,计算土地利用结构异质性指数和生态服务价值,分析土地利用结构与生态服务价值的变化及其相互关系.结果表明:1997-2006年酒泉市耕地、林地、园地面积在增加,其中耕地增加最多,增加了9 803.15hm2,牧草地、水域和未利用地面积不断减小,其中牧草地减少最多,减少了207 470.56 hm2;酒泉市土地利用结构多样性指数和均匀度增加而优势度降低,土地利用趋于多样化和均匀化;各类土地的生态服务价值都有不同程度的增加,土地利用生态服务价值总体呈增加趋势;土地利用生态服务价值与土地利用结构线性相关,通过增加牧草地、水域和耕地等生态用地面积,提高土地利用集约度,调整土地利用结构促进土地利用的多样化、均匀化,可以增加生态系统稳定性和提高生态系统服务功能的经济价值.     

The effect of mercury on trees and their mycorrhizal fungi

The Oak Ridge Reservation, established in 1942, was the designated site for the construction of the atomic bomb. During a 20-year period from 1944 to 1963 radioactive and toxic chemical pollutants, especially mercury compounds were released into the surrounding waterways. Tree diversity and mycorrhizal presence and abundance were analyzed in the mercury-contaminated floodplains of East Fork Poplar Creek Oak Ridge (EFPC) (Tennessee). A subsequent greenhouse study was conducted to assess the phytotoxic effects of different mercuric solutions on Platanus occidentalis (American Sycamore), inoculated with soils from EFPC. Total soil mercury in the field had no effect on tree diversity. Organic species of mercury proved to be more toxic than inorganic species of mercury and soil inoculants from EFPC had no protective effects against Hg toxicity in our greenhouse study. Comparison of the effects of mercury contamination in our field and greenhouse studies was difficult due to uncontrolled factors.     

生物膜水解-好氧循环系统处理活性艳蓝RB-19的微生物群落动态变化

为了研究生物膜水解-好氧循环系统处理蒽醌类染料活性艳蓝RB-19效果及其中微生物群落动态变化,利用基于16S rDNA的PCR-DGGE技术获得了微生物群落的DNA特征指纹图谱。结果表明,该系统能有效地降解活性艳蓝RB-19模拟废水,在RB-19浓度≤400 mg/L时,RB-19去除率维持在82%～96%之间,COD去除率维持在95%左右,但当RB-19浓度提高到500 mg/L时,RB-19去除率降低到58%,COD去除率降低到85%。DGGE分析表明,生物膜上的微生物群落结构随着RB-19浓度递增有显著变化,好氧、水解反应器内的细菌Shannon指数分别从1.32和1.20降低到1.11和1.19。UPGMA聚类分析和NMDS散点分析表明,水解、好氧反应器内的微生物并没有因为同处一个系统内而使得其菌群落结构产生明显的趋同倾向。系统内的多种优势菌群为兼性细菌,克隆测序的结果发现,在水解反应器存在一类具有很强还原能力的古细菌——Methanobacterium sp.MB4。