微囊藻毒素-LR的全内反射荧光免疫传感器研究

在开发全内反射荧光免疫传感器的基础上,研究水中微囊藻毒素-LR(MC-LR)的检测方法。建立了基于MC-LR平面波导免疫芯片的制备方法,并结合流动分析和荧光检测开发检测系统,针对MC-LR的免疫检测条件进行优化。结果表明,全内反射荧光免疫传感器对MC-LR抗体的检测限为0.001μg/mL;在间接竞争免疫检测模式下,最优检测条件是预反应时间5min、预反应温度37℃、进样停留时间500s;在最优检测条件下,对MC-LR的检测限为0.100μg/L,线性区间为0.200~4.000μg/L;全内反射荧光免疫传感器检测MC-LR的加标回收率均在100.0%±20.0%,平行测定的相对标准偏差小于5%。     

不同种植方式蔬菜中农药残留的差异及污染控制研究

采集了陕西地区不同种植方式的瓜果类、叶菜类蔬菜样品,研究了其中各种农药的残留情况,并分析了农药残留对环境的污染及其控制措施。结果表明:露地种植蔬菜与设施种植蔬菜相比,无论是农药残留检出率还是超标率都略高,两者的农药残留检出率分别为42.0%、36.5%,超标率分别为12.0%、8.2%;叶菜类蔬菜与瓜果类蔬菜相比,无论是农药残留检出率还是超标率都较高,两者的农药残留检出率分别为52.6%、29.6%,超标率分别为17.3%、5.4%;设施种植的瓜果类蔬菜和叶菜类蔬菜的农药残留检出率基本接近,而叶菜类蔬菜的农药残留超标率明显高于瓜果类蔬菜,露地种植的叶菜类蔬菜的农药残留检出率和超标率都远高于瓜果类蔬菜;设施种植蔬菜与露地种植蔬菜相比,甲胺磷、对硫磷、甲基对硫磷、毒死蜱、三氟氯氰菊酯和氰戊菊酯的残留检出率更高;瓜果类蔬菜与叶菜类蔬菜相比,毒死蜱、甲氰菊酯、三氟氯氰菊酯、氰戊菊酯的残留检出率更高;叶菜类蔬菜的农药施用浓度较高,施用农药后要间隔一定时间后再上市销售,并应采用更科学的施药方式,以减少农药在蔬菜中的残留和降低对环境的危害;一方面要开发并积极倡导使用高效、低毒、低残留的无公害农药,另一方面则要开发能够高效、快速降解残留农药的物质,同时还要进行农产品的清洁生产,以提高农产品的安全性,并降低环境中农药的残留危害。     

基于数据包络分析的水污染物排放指标初始分配方法与案例研究

基于数据包络分析(DEA)的点源水污染物排放指标初始分配方法,是将基于DEA的线性规划评价方法进行改进,从而使其适用于评价企业的水环境绩效,并依此进行排污指标初始分配。将非期望产出作为投入指标处理,构造了5输入1输出的规模可变DEA模型,并以南京市280家企业的COD排放指标初始分配为例进行了分析。结果表明,方法的模拟分配结果在公平的基础上,反映了企业的环境效率,效率越高,可得到的COD排放指标也越高,这将对企业治污起到激励作用,分配方法具有一定的可操作性;对案例企业进行分组并实行组内参评,基本可以避免部分极值原始数据对评价结果的影响,得到的COD排放指标分配结果也更为合理。     

地面浓度反推法计算石化企业无组织排放源强

对某石化企业储罐区和原油脱酸装置区的无组织排放非甲烷总烃进行监测,对监测数据进行Pearson相关分析,运用地面浓度反推法计算源强。实验结果表明：原油脱酸装置区各采样线浓度间的相关性好,反推出的源强较为准确,50,100,150 m采样线的反推源强非常接近,分别为21.68,21.60,19.76 t/a;而储罐区监测浓度的相关性不佳,该区域的非甲烷总烃无组织排放源可能不单一,不适用地面浓度反推法计算其源强。     

Electrochemical study of the fungicide acibenzolar-s-methyl and its voltammetric determination in environmental samples

The electrochemical behavior of new generation fungicide acibenzolar-s-methyl (S-methyl 1,2,3-benzothiadiazole-7-carbothioate, ASM) on the hanging mercury drop electrode (HMDE) was investigated using square wave adsorptive stripping voltammetry. This method of determination is based on the irreversible reduction of ASM at the HMDE. The well-defined ASM peak was observed at ?0.4 V (vs. Ag/AgCl) in BR buffer at pH 2.2. The reduction peak current was proportional to concentration of ASM from 1.0 × 10^?8 to 6.0 × 10^?8 mol L^?1 with detection and quantification limit 3.0 × 10^?9 and 1.0 × 10^?8 mol L^?1, respectively. The applicability of the developed method for analysis of spiked samples of tap water, river water, and soil is illustrated. The effect of adsorption on the mercury electrode was studied in detail using the AC impedance method. Possible interferences with other common pesticides and heavy metal ions were examined. Clarification of the electrode mechanism was made using cyclic voltammetry (CV) technique.     

Speciation and persistence of doxycycline in the aquatic environment: Characterization in terms of steady state kinetics

The aim of the present work was to establish the kinetics for the degradation of doxycycline in the aquatic environment with a view to arriving at a kinetic model that can be used to predict the persistence of antibiotic with confidence. The degradation of doxycycline in both water and sediment phases of aquatic microcosm experiments, as well as in distilled water control experiments, was studied over a period of 90 days. An initial 21% loss due to adsorption by the sediment was observed in the microcosm experiment soon after charging. Biphasic zero-order linear rates of degradation, attributed to microbial degradation of the free and sediment or colloidal particle-adsorbed antibiotic, were observed for both water phase (2.3 × 10^?2 and 4.5 × 10^?3 μgg^?1 day^?1) and sediment phase (7.9 × 10^?3 and 1.5 × 10^?3 μgg^?1 day^?1) of the microcosm experiment. The covered distilled water control experiment exhibited a monophasic zero-order linear rate (1.9 × 10^?3 μgg^?1 day^?1) attributed to hydrolysis, while the distilled water experiment exposed to natural light exhibited biphasic liner rates attributed to a combination of hydrolysis and photolysis (2.9 × 10^?3 μgg^?1 day^?1) and to microbial degradation (9.8 × 10^?3 μgg^?1 day^?1). A kinetic model that takes into account hydrolysis, photolysis, microbial degradation as well as sorption/desorption by colloidal and sediment particles is presented to account for the observed zero-order kinetics. The implications of the observed kinetics on the persistence of doxycycline in the aquatic environment are discussed.     

实验室率定法测算长江南通段污染物降解系数

污染物降解系数是确定水环境容量的关键参数之一,本文采用实验室率定法,采集长江南通段的水样,分别于6℃、10℃、16℃、20℃室温下,在实验室内进行周期为20天的室内水质分析,根据室内水质分析结果,测算出不同温度下长江南通段"十二五"减排的约束性指标化学需氧量、氨氮的降解系数,并定性分析了长江水体中污染物本底浓度、可生化性、pH值等环境因子对污染物降解系数可能发生的影响。     

天堂河河道治理工程水质改善预测分析

河道治理工程重在改善河道的水质,以恢复河流生态系统。本文以北京市大兴区天堂河河道治理工程为例,介绍了河道治理工程环境影响评价中的水质改善预测分析,首先分析生态需水量和供氧量是否满足河流生态需水要求,后又利用地面水环评助手软件预测了河流水质,预测分析了天堂河水质目标的可达性,旨在为以后的河道治理类工程环境影响评价提供参考。     

南充市城郊农村居民的环境法律意识调查与分析

本文通过采用问卷调查和走访调查的方法,对南充市两县三区的城郊农村居民进行了抽样调查。调查结果发现,当地城郊农村居民环境法律“知”的水平中等、个体差异较大,环境法律“行”的水平偏低,“知”与“行”之间存在脱节现象。这与当地城郊农村居民的文化程度和所处地域有关。为此,提出了从“知”和“行”上加强当地城郊农村居民的环境法律知识教育和环境维权意识教育的建议,这也是提高城郊农村居民环境法律意识的途径所在。     

基于因子分析和聚类分析的江苏十三市环境保护评价研究

本文根据工业废水排放量、工业二氧化硫排放量、工业烟尘排放量、工业废水排放达标率、工业固体废物综合利用率5个指标的统计数据,运用因子分析和聚类分析,对江苏13个市的环境保护状况进行评价,并将其分成5个不同的类别,第1类是苏州,第2类是南京,第3类包括无锡、南通、常州,第4类包括徐州、扬州、泰州、淮安、宿迁,第5类包括盐城、镇江、连云港,然后进一步对各类城市在污染强度和污染控制方面的特征进行分析。最后,分别对每类地区的环境保护提出对策建议。