珠江水体中细菌碱性磷酸酶基因phoD的多样性

碱性磷酸酶(APase)是最主要的有机磷水解酶,在无机磷匮乏的情况下,APase释放的无机磷供藻类利用,致使藻类大量繁殖,因此,对APase基因多样性的研究有利于揭示对水体富营养化有重要影响的微生物类群.基于此,本文以珠江水体为研究对象,构建了珠江广州段穗石、坦尾和南沙3个点细菌的pho D基因克隆文库.限制性酶切片段多态性(RFLP)分析结果表明,上述3个点的pho D基因分别含有15、17、13个操作分类单元(Operational Taxa Unit,OTU),穗石点的多样性(Shannon指数和Simpson指数)均高于其他两个点;pho D基因序列系统发育分析表明,珠江水体中pho D基因主要来源于Plesiocystis、Limnohabitans、Pirellula等属的细菌,而Limnohabitans属的pho D基因在文库中含量最高,占文库的44%~50%.研究表明,珠江水体中细菌的pho D基因具有较高的多样性,这些基因编码的碱性磷酸酶可能在珠江水体的富营养化过程中有重要的作用.     

Trophic State in Voyageurs National Park Lakes before and after Implementation of a Revised Water‐Level Management Plan

We compiled Secchi depth, total phosphorus, and chlorophyll a (Chla) data from Voyageurs National Park lakes and compared datasets before and after a new water‐level management plan was implemented in January 2000. Average Secchi depth transparency improved (from 1.9 to 2.1 m, p = 0.020) between 1977‐1999 and 2000‐2011 in Kabetogama Lake for August samples only and remained unchanged in Rainy, Namakan, and Sand Point Lakes, and Black Bay in Rainy Lake. Average open‐water season Chla concentration decreased in Black Bay (from an average of 13 to 6.0 μg/l, p = 0.001) and Kabetogama Lake (from 9.9 to 6.2 μg/l, p = 0.006) between 1977‐1999 and 2000‐2011. Trophic state index decreased significantly in Black Bay from 59 to 51 (p = 0.006) and in Kabetogama Lake from 57 to 50 (p = 0.006) between 1977‐1999 and 2000‐2011. Trophic state indices based on Chla indicated that after 2000, Sand Point, Namakan, and Rainy Lakes remained oligotrophic, whereas eutrophication has decreased in Kabetogama Lake and Black Bay. Although nutrient inputs from inflows and internal sources are still sufficient to produce annual cyanobacterial blooms and may inhibit designated water uses, trophic state has decreased for Kabetogama Lake and Black Bay and there has been no decline in lake ecosystem health since the implementation of the revised water‐level management plan.     

Model‐Based Nitrogen and Phosphorus (Nutrient) Criteria for Large Temperate Rivers: 1. Model Development and Application

An initial inquiry into model‐based numeric nitrogen and phosphorus (nutrient) criteria for large rivers is presented. Field data collection and associated modeling were conducted on a segment of the lower Yellowstone River in the northwestern United States to assess the feasibility of deriving numeric nutrient criteria using mechanistic water‐quality models. The steady‐state one‐dimensional model QUAL2K and a transect‐based companion model AT2K were calibrated and confirmed against low‐flow conditions at a time when river loadings, water column chemistry, and diurnal indicators were approximately steady state. Predictive simulation was then implemented via nutrient perturbation to evaluate the steady‐state and diurnal response of the river to incremental nutrient additions. In this first part of a two‐part series, we detail our modeling approach, model selection, calibration and confirmation, sensitivity analysis, model outcomes, and associated uncertainty. In the second part (Suplee et al., 2015) we describe the criteria development process using the tools described herein. Both articles provide a fundamental understanding of the process required to develop site‐specific numeric nutrient criteria using models in applied regulatory settings.     

中国湖库营养状态现状调查分析

近年来,随着经济的高速发展,我国湖库氮、磷污染负荷增加,富营养化问题有增无减,已经成为我国湖库面临的重大环境问题。以2005年-2007年我国大陆地区各省(自治区、直辖市)183个湖泊、541个水库的调查资料为基础,采用单项指标评价和综合评价方法,分析了我国湖库富营养化状况。结果表明:我国湖库受营养盐污染的形势十分严峻,富营养化成为我国湖库共同面临的主要环境问题;总体而言,水库的富营养状态要优于湖泊,但水库的富营养化污染也不容乐观;总磷超标情况要重于总氮,透明度超标比例相对较小。     

成都地区水质调查与富营养化评价

为了解成都地区各类水体的水质情况,以便为以后的水资源保护及水环境修复工作提供参考依据。特对成都地区的城市景观水体、住宅区景观水体、湖泊、河流以及农业养殖用水的水质情况进行调查,并采用综合营养状态指数法对各类水体的富营养化状态进行评价。结果表明:成都地区水质污染情况严重,河流和养殖水体均达到重度富营养水平,而城市水体也已接近中度富营养水平。     

上海水体营养分级的群落代谢率模型

在标准实验条件下测定天然浮游生物群落产氧率（Ｐ）和呼吸率（Ｒ）。同时结合总氮、总磷、叶绿素ａ等水质化学、生物学指标,运用方差分析、多元逐步回归分析等定量研究手段,确定Ｆ值即√Ｐ×（Ｐ／Ｒ）为代谢率的合适指标评价富营养化。在概率基础上确定Ｆ值的开发型分类,并建立了能快速、准确差别营养污染程度的差别模型。     

新丰江水库富营养化现状及其综合防治对策

新丰江水库水质可满足 Ｇ Ｂ３８３８ － ８８ Ⅱ类水质要求, 其营养水平为贫中营养状态; 磷为主要污染物; 氮磷负荷均略大于可接受的纳污负荷。本文还提出了有效控制新丰江水库富营养化进程的若干建议和措施。     

Interactions of warming and altered nutrient load timing on the phenology of oxygen dynamics in Chesapeake Bay

The effects of nutrient loading on estuaries are well studied, given the multitude of negative water quality and ecosystem effects that have been attributed to excess nitrogen and phosphorus. A current gap in this knowledge involves the sensitivity of seasonal cycles of estuarine biogeochemical processes to direct (warming) and indirect influences (nutrient load timing) of climate change. We used a coupled hydrologic–biogeochemical model to investigate changes in the phenology of hypoxia and related biogeochemical processes in Chesapeake Bay under three different hydrologic regimes. Shifts to earlier nutrient load timing during idealized simulations reduced the overall annual hypoxic volume, resulting from discernable, but relatively small reductions in phytoplankton biomass and both sediment and water-column respiration. Simulated increases in water temperature caused an increase in spring/early summer hypoxic volume associated with elevated respiration rates, but an associated exhaustion of organic matter in the early summer caused a decrease in late summer/fall hypoxic volume due to lowered respiration. Warming effects on hypoxia were larger than nutrient timing effects in scenarios where warming was restricted to spring and when it was applied to all months of the year. These idealized simulations begin the process of understanding the potential impacts of future climatic changes in the seasonal timing of key biogeochemical processes associated with eutrophication.     

滇池浮游植物碳氮同位素时空分布特征及其影响因子分析

通过对旱季、旱雨季过渡期和雨季3个不同时期滇池浮游植物 δ13C、δ15N的研究发现,滇池浮游植物δ13C、δ15N存在显著的时空分布特征.从旱季、过渡期到雨季,浮游植物δ13C值分别为-20.44‰±0.72‰、-17.32‰±1.09‰和-15.92‰±1.74‰,呈逐渐升高趋势;而浮游植物δ15N值的季节变化模式...     

Eutrophication and Consumer Control of New England Salt Marsh Primary Productivity

Abstract:  Although primary productivity in salt marshes is thought to be controlled by physical forces, recent evidence suggests that human disturbances can drive a switch to consumer control in these ecologically valuable ecosystems. We tested the hypothesis that nitrogen enrichment can trigger consumer control in salt marshes in Narragansett Bay, Rhode Island, with (1) a field experiment in which we manipulated nutrient availability (with nutrient additions) and insect herbivory (with insecticide application), (2) a survey of 20 salt marshes that examined the relationship between marsh nutrient status and herbivore pressure, and (3) insect herbivore removal at high and low nutrient input sites to directly test the hypothesis that nutrient enrichment is increasing insect herbivory in these marshes. Experimental nitrogen eutrophication initially increased plant productivity but eventually led to reduced plant biomass due to insect herbivory, and our surveys revealed that marsh nitrogen supply was a good predictor of herbivore damage to plants. Insects had minimal impacts on primary productivity in pristine marshes, but suppressed primary productivity in eutrophic salt marshes by 50–75%. Thus, eutrophication is currently triggering consumer suppression of primary productivity in New England salt marshes and may ultimately jeopardize the ecological and societal services these systems provide.