基于PCR-DGGE的重金属污染土壤微生物种群指纹分析

采用变性梯度凝胶电泳（DGGE）分析方法,研究沈阳市细河沿岸重金属污染土壤中微生物结构多样性、种群丰富度及种群结构的动态变化规律。结果表明：直接从土壤中抽提总DNA,对16SrRNAV3可变区序列进行扩增,DGGE指纹图谱分析,微生物种群结构和变化规律明显,同时也表现为重金属污染程度越重,多样性指数越低;其中细河底泥重金属含量严重超标,多样性指数最低。经方差分析,重金属污染情况与Shannon-wiener多样性指数呈负相关,其中重金属污染对微生物的均匀度指数影响最显著,并且锌污染对其影响最大为-0.985。表明PCR-DGGE技术可以用于污染环境下微生物多样性研究,也为污染环境下土壤微生物多样性研究提供了新的实验方法与依据。     

水深梯度对荆三棱(Scirpus yagara)生长的影响

研究湿地植物对水深梯度的响应对于湿地修复具有重要的指导意义.在南四湖湖边滩地上的人工修复湿地中开展水深梯度对荆三棱(Scirpus yagara)生长的影响研究,试验观测水深分别为-10、0、10、20、30 cm共5个梯度.结果显示:随着水位的升高,1)荆三棱的株高、基茎、叶长和叶宽均有所增加,当水位大于10 cm时各项指标增加更为显著;2)生物量呈增加趋势,而根茎比则逐渐减小,水位为30 cm时的生物量和根茎比分别为-10 cm时的2.67倍和58.15%;3)叶绿素含量总体呈增加趋势,但在不同水位间的差异不明显(P>0.05);4)叶绿素荧光方面,最大光化学量子产量Fv/Fm均在0.75以上,光化学淬灭qP和非光化学淬灭qN分别呈现出升高和下降的趋势.结果表明,在试验所设置的水位条件下(-10～30 cm),荆三棱的生长没有受到水深梯度增加的显著影响,相反表现出随着水深的增加而生长更好的趋势.可见,在试验设置的水深梯度范围内,相对较高的水位有利于荆三棱的生长,因此在湿地管理中应控制适当的高水位以利于其种群的生长和稳定.     

Estimation of atmospheric trace metal emissions in Vilnius City, Lithuania, using vertical concentration gradient and road tunnel measurement data

A new approach for the estimation of trace metal emissions in Vilnius city was implemented, using vertical concentration profiles in the urban boundary layer and road tunnel measurement data. Heavy metal concentrations were examined in fine and coarse particle fractions using a virtual impactor (cut-off size diameter 2.5 μm). Negative vertical concentration gradients were obtained for all metals (Ba, Pb, V, Sb, Zn) and both fractions. It was estimated that the vertical concentration gradient was formed due to emissions from an area of about 12 km². Road tunnel measurements indicated that trace metal concentrations on fine particles were lower than those on coarse particles, which suggested that re-emitted road dust was highly enriched in trace metal due to historic emissions within the tunnel. Emission rates of different pollutants in the road tunnel were calculated using pollutant concentration differences at the tunnel entrance and exit and traffic flow data. Heavy metal emission rates from the area of Vilnius city were estimated using the vertical gradient of heavy metal concentrations and the coefficient of turbulent mixing, as derived from meteorological measurement data. The emission values calculated by the two different methods coincided reasonably well, which indicated that the main source of airborne trace metals in Vilnius city is traffic. The potential of the vertical concentration gradient method for the direct estimation of urban heavy metal emissions was demonstrated.     

EVALUATION OF THE ROSGEN STREAM CLASSIFICATION SYSTEM IN CHEQUAMEGON‐NICOLET NATIONAL FOREST,WISCONSIN1

ABSTRACT: Data collected from 121 stream reaches during 1991 to 1993 were evaluated to determine the applicability of the Rosgen Stream Classification System (RSCS) to the low relief terrain within the Chequamegon‐Nicolet National Forest (CNNF) in Wisconsin, USA. All reaches were classified to RSCS Level I and II except that 10.7 percent had sinuosities below the continuum limits and one reach had a predominantly organic substrate. Five of eight possible RSCS Level I types were observed including B, C, D, A, E, and F; 86 percent were C and E types. Seventeen of 94 possible RSCS Level II types were observed. Most reaches were slightly entrenched, had low to moderate width/depth ratios, relatively low sinuosity, low slope, and sand or gravel as the dominant channel material. Discriminant analyses were used to verify the applicability of RSCS for streams within the CNNF; discriminant functions correctly classified 92.5 and 94.7 percent of the Level I and II RSCS types, respectively. When limits for E and F types were modified slightly at Level II by adding an additional category for slopes less than 0.1 percent (a modification we recommend for low relief terrain), discriminant functions correctly classified 99.1 percent of the types. Adding another slope break at 0.3 percent produced similar results. Based on our analyses, RSCS works well within the CNNF and is probably applicable to other areas with low‐relief terrain.     

快速木质纤维素分解菌复合系MC1对秸秆的分解能力及稳定性

以天然水稻秸秆为材料研究了快速降解木质纤维素的细菌复合系MC1对木质纤维素的分解能力;并在不同条件保藏、高温处理以及利用变性梯度胶电泳(DGGE)技术研究了复合系的稳定性.结果表明,复合系MC1在50℃液体静止培养条件下8～10d,把培养液2%干重的水稻秸秆完全分解溶化;经过9d的培养,水稻秸秆的总干重减少81%,其中纤维素减少99%,半纤维素减少74%,木质素减少51%.连续继代培养4a、常温干燥保存4a、-20℃冷冻藏4a、培养液直接在室温和4℃保存1a、90℃处理30min仍具旺盛的分解能力并稳定传代.平板培养基培养证明MC1全部由细菌组成,16SrDNA变性梯度胶电泳(DGGE)检测结果,在6个月内主要条带几乎没有变化,说明MC1的菌种组成相当稳定.MC1对纤维素的分解利用具重要前景.     

昆明市经济发展梯度及经济一体化发展策略

昆明市下辖14个县(市)区,14个县(市)区之间经济发展水平差异显著。建立了昆明市经济发展水平评价指标体系,利用聚类分析法结合2006年《昆明统计年鉴》以及2006年《云南省统计年鉴》数据,将14个县(市)区划分为3个经济梯度,并提出了梯度间一体化发展策略。     

Community analysis in stream biomonitoring: what we measure and what we don't

Diatom assemblages from 83 epilithic samples taken from the Mesta River, Bulgaria, were regressed against three sets of predictor variables, i.e. environmental, spatial, and temporal. Redundancy analysis (RDA) of species and environmental data explained 36% of the diatom variance and extracted several important gradients of species distribution, associated with a downstream increase in nutrient levels, pH, temperature, and organic pollution. The inclusion of spatial and temporal variables in the RDA model captured additional 24% of the diatom variance and revealed three more gradients, a spatial gradient represented by higher order polynomial terms of latitude and longitude, and two temporal gradients of annual and seasonal variation. Partial RDAs demonstrated that the unique contribution of each predictor set to the explained diatom variance was the highest in the spatial dataset (16%), followed by the environmental (9%), and the temporal (7%) datasets. The remaining 28% of the variance was explained by the covariance of the predictor sets. This suggests that in biomonitoring of single stream basins, the cheap and simple account of space and time would explain most of the variance in assemblage composition obviating the necessity of expensive and time-consuming environmental assessments. The nature of the underlying environmental mechanisms can be easily inferred from the diatom composition itself.     

Gradient analysis of the chrogenetic structure of lowland and highland scotch pine populations

Gradients of genetic distances (GGDs) between 26 adjacent cenopopulations of Scotch pine (Pinus sylvestris L.) along the transects through the distinct landscape-ecotopic isolation borders were analyzed graphically. The results made it possible to reveal and quantitatively estimate gradients and borders of gene-pool structural patterns in populations of this species in the central part of Northern Eurasia. In lowland areas, the most distinct borders were found between pine populations growing on dry lands and bogs (Pineta sphagnosa) in the forest zone of the Transural region, as well as between the Arakaragaiskii and Amankaragaiskii island pine forests in the steppe zone (Northern Turgai). In highland areas (the Urals and the Carpathians), the greatest GGDs were observed between low-mountain (about 600 m above sea level) and middle-mountain (850–900 m) populations. Analysis of GGDs is a promising gene-geographic method for determining population borders and studying the chorogenetic structure of species.     

生物多样性的海拔分布格局研究及进展

生物多样性的海拔分布格局受制于气候、空间、环境等多种因子的影响,综合大量研究发现,无论是动植物还是微生物,环境因子对其影响与驱动的作用最明显.海拔梯度是决定分布格局的重要因素之一,因此探讨生物多样性在环境因子驱动下的海拔分布格局具有重要意义.文章分别探讨了植物、土壤动物和土壤微生物多样性沿海拔梯度的变化规律,揭示了温度...     

基于风险梯度的化工企业事故风险定量评估

为了定量分析区域内重大危险源所构成的风险,为事故风险防控提供科学依据,基于风险场理论对某区域内2家化工企业进行风险分析,得出2家企业所对应的风险值计算公式,并生成2家企业所对应的风险等值面和风险等值线;引入最速下降法,利用负梯度搜寻风险的最快下降方向,得出事故的最优风险降低路径。研究结果表明:甲企业中接近1/2的区域为Ⅲ级风险区域,其余区域为Ⅱ级风险区域,乙企业中大部分区域为Ⅱ级风险区域,仅少部分区域属于Ⅲ级风险区域。相较而言,乙企业中5条最优风险降低路径里程更短,人员分配情况更为合理,更具安全优势;甲企业事故风险更大,更需要完善相关安全措施以免发生事故。