公众参与市容环境规划与管理的方法设计--以南京市的实践为例

市容环境是与公众生活联系最为紧密的领域,也是公众参与最容易进行的领域。南京的城市建设要实现“经济发达、环境优美、融古都特色和现代文明于一体的现代化江滨城市”的目标,公众参与市容环境的规划与管理是关键内容之一。论文以“南京市市容环境卫生发展规划”项目工作为基础,分析和阐述公众参与的内涵,提出公众参与市容环境的五大步骤,并针对南京市容环境方面的具体案例,设计出符合南京特色的公众参与市容环境规划与管理的方法框架模型,包括决策模式、制度框架的共管体系。     

长三角快速城市化地区土地利用变化对生态系统服务价值的影响——以嘉兴市为例

基于浙江省嘉兴市1979~2012年的土地利用数据,评估了各种土地利用类型的生态系统服务价值,分析了30多年来嘉兴市土地生态系统服务价值的动态变化及其对土地利用变化和景观格局变化的响应。结果表明:(1)1979~2012年,嘉兴市建设用地面积变化最大,增加了136.6%,相应的耕地面积减少了15.5%。(2)1979~2012年,嘉兴市景观破碎度指数从0.6531上升到0.8762,随着城市化进程的加剧,耕地景观破碎度指数不断增加、聚集度指数减小。(3)1979~2012年,嘉兴市的总生态系统服务价值减少了14.7%,2000~2012年嘉兴市总的生态系统服务价值减少尤为显著,生态系统各单项服务价值整体也呈现下降趋势,耕地为嘉兴市提供的生态系统服务占嘉兴市总生态系统服务的70%以上。(4)运用相关分析方法对嘉兴市土地利用类型和景观格局对土地生态系统服务价值的影响分析表明,耕地和水域与生态服务价值呈正相关,而建设用地和未利用地与生态服务价值呈负相关;景观多样性指数(SHDI)和耕地破碎度指数与生态系统服务总价值呈显著负相关关系。     

基于MSPA与MCR模型的余江县生态网络构建基于MSPA与MCR模型的余江县生态网络构建

在我国社会经济快速发展的背景下,城市化进程的不断推进导致城市生态斑块日益减少,生态环境问题正在逐年增加,构建生态网络是改善城市生态环境与服务功能的有效方法之一。以余江县为研究区,采用形态学空间格局分析(MSPA)方法和景观指数法,提取对生态网络具有重要意义的生态源地,并基于MCR模型构建综合阻力面,采用最小成本路径方法生成潜在廊道,再基于重力模型、中介中心度等对关键廊道及踏脚石斑块进行识别并提取,从而构建研究区潜在生态网络。结果表明：MSPA方法能够与MCR模型有机的结合,通过定量的分析识别出研究区潜在生态廊道,并根据斑块的中介作用选定踏脚石斑块,明确研究区景观要素的保护优先度,将景观中的潜在生态源地及廊道作为生态网络构建的主要依据,能够更加科学地为余江县生态网络的构建提供指导,同时也可为其他区域提供参考。     

Evaluating unintended consequences of intentional species introductions and eradications for improved conservation management

Increasingly intensive strategies to maintain biodiversity and ecosystem function are being deployed in response to global anthropogenic threats, including intentionally introducing and eradicating species via assisted migration, rewilding, biological control, invasive species eradications, and gene drives. These actions are highly contentious because of their potential for unintended consequences. We conducted a global literature review of these conservation actions to quantify how often unintended outcomes occur and to elucidate their underlying causes. To evaluate conservation outcomes, we developed a community assessment framework for systematically mapping the range of possible interaction types for 111 case studies. Applying this tool, we quantified the number of interaction types considered in each study and documented the nature and strength of intended and unintended outcomes. Intended outcomes were reported in 51% of cases, a combination of intended outcomes and unintended outcomes in 26%, and strictly unintended outcomes in 10%. Hence, unintended outcomes were reported in 36% of all cases evaluated. In evaluating overall conservations outcomes (weighing intended vs. unintended effects), some unintended effects were fairly innocuous relative to the conservation objective, whereas others resulted in serious unintended consequences in recipient communities. Studies that assessed a greater number of community interactions with the target species reported unintended outcomes more often, suggesting that unintended consequences may be underreported due to insufficient vetting. Most reported unintended outcomes arose from direct effects (68%) or simple density-mediated or indirect effects (25%) linked to the target species. Only a few documented cases arose from more complex interaction pathways (7%). Therefore, most unintended outcomes involved simple interactions that could be predicted and mitigated through more formal vetting. Our community assessment framework provides a tool for screening future conservation actions by mapping the recipient community interaction web to identify and mitigate unintended outcomes from intentional species introductions and eradications for conservation.     

环境样品中亚硝酸细菌amoA基因的克隆与测序

对从环境样品中分离的亚硝酸细菌(Ammonia oxidizingbacteria)amoA基因进行克隆与测序,为构建基因工程菌打下基础。采用亚硝酸细菌选择性培养基,从4个不同的畜牧养殖污水处理厂采集的样品(分别编号为1,2,3,4)在室温下富集培养2个月后,采取酚氯仿抽提的方法提取DNA。根据已报道的亚硝化单胞菌(Nitrosomonassp.)amoA基因序列,设计引物AMOB AMOE,并在AMOB,AMOE的5′-端分别加上了BamHⅠ和HindⅢ的限制性酶切位点,以利于进一步酶切和克隆。用AMOB AMOE对4种样品的DNA进行PCR扩增,PCR产物进行琼脂糖凝胶电泳分析。结果表明,4种样品中1号和3号样品扩增得到预期长度的DNA片段,2号和4号样品扩增没有得到预期片段。回收纯化PCR产物与pGEM-T载体连接,构建amoA基因测序载体,并转化E.coliM15。测序结果提交GenBank进行Blast分析。结果显示,扩增得到的DNA片段均与Nitrosomonassp.GH22的amoA基因有99 7%的同源性,可从环境中分离的亚硝酸细菌中克隆出amoA基因。      

韶石山风景区岩石中的名胜古迹

韶石古道是古代上京古道的一段。韶石山是唐宋以来的旅游胜地，岩洞、崖壁中遗留众多的名胜古迹，如岩庙、石寨、石刻石雕、岩画、岩棺墓等珍贵的历史遗迹。     

土地利用变化对湿地景观连通性的影响及连通性优化效应——以江苏盐城海滨湿地为例

土地利用与土地覆盖变化对湿地景观的结构和功能产生深刻的影响。选择江苏盐城海滨地区为研究对象,应用遥感和地理信息系统技术,分析20年来土地利用变化对湿地景观连通性的影响,并以2007年湿地景观生态系统服务功能为基础,采用阻力面模型探讨湿地景观连通性优化途径及其效应。结果显示:①1987—2007年间,盐城海滨区域土地利用结构变化显著。其中耕地面积比重由36.72%上升为46.17%,自然湿地持续减少,面积由44.4%减为26.01%,人工湿地持续增加,面积由9.96%上升为18.72%;②随着区域人类土地利用活动的加强,光滩、碱蓬沼泽和芦苇沼泽的空间连通性降低,区域土地利用导致湿地景观之间生态流阻隔,景观生态服务功能减弱;③2007年湿地景观生态功能强度空间差异显著,以累积耗费距离面、生态源地、耗费路径为依据,对湿地景观连通路径优化结果表明,废黄河口和大丰港附近等关键区域对景观连通性和生态流影响最大,是景观生态节点优化的首要对象;④加强连通路径的关键区域优化、提高景观连通度是实现景观优化的关键。     

规划环评中景观非生态功能影响评价实例

以景观概念作为融合自然与社会、科学与美学、理性与感性的结合点,研究了景观非生态功能的内涵及其评价技术理论框架,从景观功能中提炼出景观非生态功能概念,初步完成景观非生态功能评价指标体系的构建.评价技术体系设计突出环境影响评价中现状评价的客观性以及影响预测的科学性.以人感尺度下的评价对象——景观单元概念为创新点,该评价体系补充并完善了现有景观评价体系的技术链条,在保证评价精度的前提下,简化并规范了评价工作流程.该评价体系沿用现有环境影响评价所广泛采用的现状评价、影响源分析、影响预测评价及措施建议的技术思路,基本能满足现行技术规范的要求.以武汉市后官湖生态宜居新城规划环评为例,验证了该体系的可操作性,为我国规划环评在景观评价技术领域的发展提供理论和实践参考.      

Reference gene validation for quantification of gene expression during final oocyte maturation induced by diethylstilbestrol and di-(2-ethylhexyl)-phthalate in common carp

Final oocyte maturation is the key step to successful spawning and fertilization.Quantitative real-time PCR(q PCR) is the technique of election to quantify the abundance of functional genes in such study. Reference gene is essential for correct interpretation of q PCR data. However, an ideal universal reference gene that is stable under all experimental circumstances has not been described. Researchers should validate their reference genes while performing q PCR analysis. The expression of 6 candidate reference genes: 18 s r RNA,28 s r RNA, Cathepsin Z, Elongation factor 1-α, Glyceraldehyde-3-phosphate dehydrogenase andβ-actin were investigated during final oocyte maturation induced by different compounds(DES and DEHP) in common carp(Cyprinus carpio). Four softwares(Bestkeeper, ge Norm,Norm Finder and Ref Finder) were used to screen the most stable gene in order to evaluate their expression stability. The results revealed that EF1α was highly stable expressed when final oocyte maturation was induced by DES, while gapdh was the most stable gene when final oocyte maturation was induced by DEHP. Stable expressed reference gene selection is critical for all q PCR analysis to get accurate target gene m RNA expression information.     

基于景观生态学的生态功能区划研究——以重庆市长寿区为例

针对目前生态功能区划仅从区域生态要素、生态敏感性与生态服务功能空间分异规律等方面考虑,而很少涉及景观格局特征与景观稳定度等景观生态学的内容;文章在景观生态学理论基础上,提出一套基于景观生态学的区域生态功能区划理论;一方面有助于生态功能小区的划分;另一方面可以弥补原有生态保育措施只考虑生态敏感性和生态服务功能重要性的不足,从景观生态学角度提出具有针对性的生态保育措施。文章将所建立的理论与方法用于重庆市长寿区生态功能区划,区划结果为:将重庆市长寿区划分为3个生态亚区,9个生态功能区和21个生态功能小区,与未考虑景观稳定度的生态功能区划相比,考虑景观稳定度的生态功能区划多了四个生态功能小区,而这四个生态功能小区的划出,有助于进一步针对具体问题,从生态学和景观生态学的角度提出生态保育措施,对重庆市长寿区环境保护和生态建设具有重要的指导意义。