TCDD对乳腺癌细胞端粒酶活性的影响

探讨了2,3,7,8-四氯二苯并-ρ-二噁英(TCDD)在体外对雌激素依赖性乳腺癌细胞MCF-7端粒酶活性的影响.用0.5μmol·L-1的2,3,7,8-四氯二苯并-ρ二噁英(TCDD)作用于MCF-7细胞,裂解后提取MCF.7细胞端粒酶.采用BCA方法定量蛋白、重复序列扩增-银染法检测端粒酶活性.结果表明.TCDD作用MCF-7细胞后,细胞端粒酶活性增强,且随作用时间端粒酶活性减弱,但明显高于对照组(P<0.05).结果表明:TCDD有显著增强MCF-7细胞端粒酶活性的作用.     

连城太阳能光伏产业发展现状与战略选择

太阳能光伏产业是21世纪备受世界各国重视的高新技术产业之一,拥有广阔发展前景,基于对太阳能光伏产业链、国内太阳能光伏产业现状的分析及连城太阳能光伏产业现状与面临挑战的分析,提出连城太阳能光伏产业发展战略。     

CDM potential of SPV lighting systems in India

In view of the increasing interest in the development and dissemination of technologies for harnessing new and renewable sources of energy in India, there have also been some efforts towards their use in the domestic lighting sector. However, the cumulative number of Solar Photovoltaic (SPV) lighting systems such as SPV lanterns and solar home lighting systems in India is far below their theoretical potential despite government subsidy programmes. One of the major barriers is the high capital investment in these systems. The Clean Development Mechanism (CDM) provides industrialized countries with an incentive to invest in emission reduction projects in developing countries to achieve a reduction in CO₂ emissions at lowest cost that also promotes sustainable development in the host country. SPV lanterns and solar home lighting systems could be of interest under the CDM because they directly displace greenhouse gas emissions while contributing to sustainable rural development. However, only two SPV projects have been submitted under the CDM so far. This study assesses the maximum theoretical as well as the realistically achievable CDM potential of SPV lanterns and solar home lighting systems in India. The SPV lantern project is financially viable at a certified emissions reductions (CER) price of 34 € whereas the solar home lighting project is financially viable at a CER price of 46 €. While the maximum mitigation volume is about 35 million tonne CO₂ on an annual basis, an estimate of achievable CER levels is done using the past diffusion trends of SPV systems. We find that annual CER volumes could reach 0.8 to 2.4 million by 2012 and 5.6 to 13.6 million by 2020. This would require that the government sets the subsidy level for SPV lighting systems at a level that allows them to become viable with the CER revenue. From a macro-economic point of view this makes sense if the sustainability benefits are deemed sufficiently high to warrant promotion of this type of project.

牛蛙生长激素基因在COS7细胞中的转染表达

应用RT-PCR技术克隆牛蛙生长激素(BfGH)的cDNA,T-A克隆法构建反向插入的pMD18-T/BfGH重组质粒,回收BamHⅠ酶切的BfGH cDNA片段,并与去磷酸化处理的真核表达载体VR1020/BamHⅠ,酶切鉴定方法筛选BfGH正向插入的重组真核表达质粒VBfGH.脂质体法介导质粒VBfGH转染哺乳动物细胞COS7,对转染后的COS7细胞进行RT-PCR、ELISA和免疫荧光检测,分别在转录和翻译水平证实BfGH基因在COS7细胞中得到了正确的转染表达.图4表1参17     

AzaC预处理增加TCDD对特殊细胞P450基因的诱导

利用RT-PCR检测不同物质诱导细胞的CYP基因 mRNA的表达水平.在HepG2细胞,TCDD能诱导CYP1A1、CYP1B1及CYP1A2基因表达,CYP1A1、CYP1B1基因比CYP1A2基因更容易被诱导,用AzaC处理后CYP1B1基因表达无改变;在A549细胞和SPC-A1细胞,Azac预处理后增加了TCDD对CYP1家族的诱导.也就是AzaC增加了CYP1A1、CYP1A2和CYP1B1基因表达水平.图1表1参10     

高山植物唐古特红景天粘液细胞及叶肉细胞表面糖蛋白与抗冻性的关系

分布于青海高原海拔4000m高山的唐古特红景天有很强的抗冻能力，其叶片半致死温度可达－26.5℃。在北京地区夏季（7月）驯化20d后，长出新叶片的半致死温度升高为－15.5℃。光镜组织化学切片显示，在唐古特红景天叶片中，特别是维管组织周围，存在多糖、蛋白质或糖蛋白含量丰富的粘粹细胞；高温驯化后长出的新叶片这种粘液细胞明显减小或消失。电镜细胞化学研究揭示，在细胞壁外层及细胞间隙中分布着明显的糖蛋白，在北京地区驯化20d的唐古特红景天叶片中这种糖蛋白明显减少。因此推测，粘液细胞和叶肉细胞表面的糖蛋白层，对唐古特红景天抗冻能力可能起着重要的作用，图版1图2参20。     

吸烟烟气对鼠肺细胞膜的损伤和茶多酚的保护作用

本文以香烟气相物质作用鼠肺细胞膜为模型,用脂肪酸自旋标记物5-DOXYL和16DOXYL分别研究膜浅层和深层的动态性质受气相烟的影响,并用紫外可见分光光度法研究气相烟对膜脂的作用。结果发现,在实验的气相烟流量下,香烟气相物质能引发鼠肺细胞膜的脂质过氧化,并且使膜浅层的流动性增大。但对膜深层的动态性质没有明显的改变,如果在鼠肺细胞中预先加入粗晶态或粉态茶多酚。则肺细胞的过氧化和膜的动态性质改变受到抑制,而且这种抑制作用与茶多酚浓度呈量效关系,而茶多酚本身对膜浅层无明显作用,但对膜深层的流动性有一定影响,而且两种茶多酚的作用相似。     

The Nile delta-Alexandria coast: vulnerability to sea-level rise,consequences and adaptation

Like many delta systems, the coastal zoneof the Nile delta has been designated as avulnerable zone to a rising sea level as aconsequence of expected climate changescombined with geological and human factors.In view of the understanding of thesefactors, a degree of vulnerabilityanalysis has been carried out to betterlocate which sectors need to be assessedand adapted to possible sea level rise(SLR) for the Nile delta-Alexandria region of Egypt.Results reveal that not all of the coastalzones of the Nile delta are vulnerable toaccelerated sea-level rise at the samelevel. Based on multiple criteria the Niledelta-Alexandria coast can be categorizedinto vulnerable (30%), invulnerable (55%)and artificially protected coastalstretches (15%). These criteria include:local subsidence or uplifting, relativesea-level rise (RSLR), land topography,width of lagoon barriers, beach-face slope,high-elevated features such as dunes andridges, eroding and accreting coastlinesand protection works.Moreover, this study evaluates thelong-term relative sea-level rise andsubsidence rates along the Nile delta andAlexandria coasts. Statistical analysis oflong-term tide gauge data recorded atAlexandria, Burullus and Port Said yieldsvalues of 1.6, 1.0 and 2.2 mm/yr,respectively. These values of relativesea-level rise and long-term subsidencerate obtained from age-dated sediment coresections are inconsistent: long-termsubsidence appears to be larger (maximum of7 mm/ yr). This discrepancy might beexplained if the subsidence is episodic,and occurs rather abruptly during majorearthquakes that occur every few hundredyears associated with fault trend lines.Rising sea levels could have significantlongterm impacts on the Nile delta,including the distribution of ground watersalinity and erosion of the narrow andlow-lying barriers of the Burullus andManzala lagoons. Adaptive measures alongthe study area particularly those relatedto coastal protective structures are alsoevaluated.     

中国沿海常见棘毒鱼类的毒性研究：日本鬼背鳍棘中Ⅰ型毒腺细？…

皮光、电镜方法研究了日本鬼Ｙｏｕ背鳍棘中Ⅰ型毒腺细胞的形成过程。结果表明：位于背鳍棘两侧纵沟内的毒腺组织周围有较致密的结缔组织的鞘膜包绕，该鞘膜钝性剥离后可见内面光滑，有Ⅰ型和Ⅱ型型毒腺细胞的痕迹。而毒腺组织内下侧与毒棘的骨组织相连处结缔组织疏松，有许多小的梭形细胞；在梭形细胞之间及周围有新形成的幼稚腺细胞，其组织结构类似Ⅰ型毒腺细胞。透射电镜下可见该区域的结缔组织中除结缔组织的细胞及成分以外，有     

Molecular evidence of fetal-derived chromosome 21 markers (STRs) in transcervical samples

Transcervical cells (TCCs), collected by flushing or aspiration at 8–13 weeks of gestation, were analysed for the presence of fetal-derived DNA sequences. DNA extracted from maternal peripheral blood, TCC samples, and placental tissue was amplified by the polymerase chain reaction (PCR) to detect small tandem repeat (STR) markers specific to chromosome 21. STR products of fetal origin could be clearly observed in four TCC samples. TCC samples collected by flushing or aspiration were also analysed by fluorescent in situ hybridization (FISH) using X and Y probes simultaneously: 46,XY cells could be detected in all TCC samples obtained from mothers with male fetuses.