Effects of a lead- and cadmium-resistant fungal strain on the absorption of heavy metals by Vetiveria zizanioides北大核心CSCD

A two-factor randomized complete block experiment was used to explore the remediation by a plant-microorganism combination on soils contaminated by lead (Pb) and cadmium (Cd). Factor A was the amount of fungi, for which four values were considered, namely, 0, 1, 3, and 5 g. Factor B was the level of contamination by lead and cadmium, for which six values were considered, namely, Cd0Pb0, Cd10Pb400, Cd20Pb600, Cd30Pb800, Cd50Pb1200, and Cd80Pb1800 (data in units of mg/ kg). The results showed that the resistant fungi promoted the growth of vetiver (Vetiveria zizanioides). At weights of 1, 3, and 5 g, the resistant fungi increased the biomass of vetiver by 41.9%, 74.9%, and 71.7% respectively. The resistant fungi stimulated the absorption of lead and cadmium by both the aerial and underground parts of vetiver. In the presence of 80 mg/kg of Cd2+ and 1 800 mg/kg of Pb2+, the contents of lead in the aerial parts of vetiver were increased by 120.6%, 265.4%, and 242.9%, while the lead content in the underground parts were increased by 110.3%, 278.2%, and 266.2%, after the addition of 1 g, 3 g, and 5 g of fungi, respectively. The content of cadmium in the aerial parts increased by 113.2%, 238.3%, and 217.3%, while the content of cadmium in the underground parts increased by 103.1%, 298.8%, and 274.4%, after the addition of 1 g, 3 g, and 5 g of fungi, respectively. The addition of fungi strengthened the effect of V. zizanioides to remediate soils contaminated by lead and cadmium, and the remediation after the addition of 3 g of fungi was better than that after treatment with 1 g and 5 g of fungi. The combination of resistant fungi and the heavy metal enrichment plant, vetiver, under different concentrations of lead and cadmium showed that the fungi had a significant effect on the remediation of soils contaminated by lead and cadmium. © 2018 Science Press. All rights reserved.     

四川土荆芥精油对植物病原真菌的抗菌活性

以植物病原真菌玉米纹枯病菌Rhizoctonia solani Kuha、玉米弯胞病菌Curvularia lunata（walk）Boed、水稻稻瘟病菌Pyricularia oryzae Cav.、小麦赤霉病菌Gibberellezeae（Schw.）Petch和松赤枯病菌Pestalotia funerea为实验菌,检测了四川土荆芥Chenopodium ambrosioidesL.精油的抑菌活性和抑菌机理。结果表明：培养基给药时,土荆芥精油对5种植物病原真菌均具有抑制作用,IC50分别为：0.734μL·mL-1,0.342μL·mL-1,0.435μL·mL-1,0.370μL·mL-1和0.332μL·mL-1;熏蒸给药时,精油剂量为1μL,土荆芥精油对松赤枯病菌、水稻稻瘟病菌、玉米纹枯病菌和玉米弯胞病菌具有促进作用,高剂量给药对菌丝生长具有抑制作用。半抑制体积分数的土荆芥精油作用于植物病原真菌,菌丝DNA不发生改变,菌丝体结构被破坏,原生质体聚集,蛋白质缺失或增加。     

丛枝菌根真菌对鸢尾的促进作用研究

为高效利用水陆两栖植物鸢尾修复污染水体,本研究通过测定不同的丛枝菌根真菌（AMF）与鸢尾构建共生体系的生长指标、土壤理化性质及植物光合作用指标,探讨不同AMF对水生植物鸢尾的促进作用。结果表明：AMF对鸢尾的促进作用主要体现在地上及地下两部分,其中地下部分通过利用其庞大的菌丝网络吸收土壤中的营养物质,进而促进了鸢尾的生长,其中对比无菌剂侵染的空白植物,摩西球囊霉作用的鸢尾对氮元素的吸收率提高71.75％,磷元素的吸收率提高8.36％,而根内球囊霉作用的鸢尾对氮元素的吸收率提高42.55％,磷元素的吸收率提高9.5％;而地上部分则是通过加强叶片气孔导度的开启来调控植物净光合速率与蒸腾速率之间的平衡,进而提高了鸢尾的最优水资源利用率,加快植物的新陈代谢,最终促进植物的生长发育。其中对于鸢尾光合作用的调节摩西球囊霉的促进效果显著好于（P＜0.05）根内球囊霉。     

Evaluation of heavy metal concentrations of edible wild-grown mushrooms from China

ABSTRACT

The heavy metal contents (Co, Cu, Fe, Mn, Ni, and Zn) of eight species of wild edible mushrooms from China were determined. The analyses were performed using inductively coupled plasma atomic emission spectrophotometry after microwave digestion. The contents of Co, Cu, Fe, Mn, Ni, and Zn in caps of mushroom samples were 0.7–7.2, 16.2–70.4, 371–1315, 12.5–29.8, 7.1–58.5, and 77.8–187.4 mg kg^?1 dry matter (dm), respectively, while considerable differences were found to be 1.8–25.9, 9.8–36.3, 288–6762, 13.3–103.9, 5.9–78.7, and 38.7–118 mg kg^?1 dm for stipes. The results indicated that higher levels of Co, Fe, and Ni were found in the mushrooms samples analyzed. Zinc and manganese levels were similar to previous reports, whereas Cu was lower than literature values. Correlation analysis suggested that significant correlations were found between the minerals determined and the greatest amount of contamination is associated with Co, Mn, Ni, and Fe. The results of this study indicate that heavy metal contents in mushroom species are mainly related to the mineral resources of sampling sites.     

Interactions of Trametes versicolor, Coriolopsis rigida and the arbuscular mycorrhizal fungus Glomus deserticola on the copper tolerance of Eucalyptus globulus

The presence of high levels of Cu in soil decreases the shoot and root dry weights of Eucalyptus globulus. However, higher plant tolerance of Cu has been observed in the presence of the arbuscular mycorrhizal (AM) fungus Glomus deserticola. The hyphal length of G. deserticola was sensitive to low Cu concentrations, and the percentage of AM root colonisation and the metabolic activity of the AM fungus were also decreased by Cu. Therefore, a direct effect of Cu on the development of the AM fungus inside and outside the root cannot be ruled out. E. globulus colonised by G. deserticola had higher metal concentrations in the roots and shoots than do non-mycorrhizal plants; however, the absence of a higher root to shoot metal ratio in the mycorrhizal plants (1.70 ± 0.11) indicated that G. deserticola did not play a filtering/sequestering role against Cu. The saprobe fungi Coriolopsis rigida and Trametes versicolor were able to remove Cu ions from the asparagine–glucose growth media. However, plants inoculated with C. rigida and T. versicolor did not accumulate more Cu than non-inoculated controls, and the growth of the plant was not increased in the presence of these fungi. However, C. rigida increased the shoot dry weight, AM root length colonisation, and metabolic mycelial activity of plants colonised with G. deserticola in the presence of Cu; only this saprobe-AM fungus combination increased the tolerance of E. globulus to Cu. Inoculation with G. deserticola and C. rigida increased the E. globulus Cu uptake to levels reached by hyperaccumulative plants.     

Microbial transformation of synthetic estrogen 17α-ethinylestradiol

Natural estrogens such as estrone, 17β-estradiol, estriol, and the particularly recalcitrant synthetic estrogen 17α-ethinylestradiol used as oral contraceptive, accumulate in the environment and may give rise to health problems. The processes participating in their removal from soil, wastewater, water-sediments, groundwater-aquifer material, and wastewater or sewage treatment plant effluents may involve the action of bacterial and microbial consortia, and in some cases fungi and algae. This review discusses the different efficiencies of bacterial degradation of 17α-ethinylestradiol under aerobic and anaerobic conditions, the role of sulfate-, nitrate-, and iron-reducing conditions in anaerobic degradation, and the role of sorption. The participation of autotrophic ammonia oxidizing bacteria and heterotrophic bacteria in cometabolic degradation of estrogens, the estrogen-degrading action of ligninolytic fungi and their extracellular enzymes (lignin peroxidase, manganese-dependent peroxidase, versatile peroxidase, laccase), and of algae are discussed in detail.     

Fungi Associated with Degradation of Wastes from Rubber Processing Industry

A study of fungi associated with wastes from a rubber processing factory was carried out. Rubber processing wastes, natural rubber waste serum (NRWS) and washing effluents were obtained from Greenpark Rubber Industries Ltd, Umutu, Delta State, Nigeria. The NRWS was collected from seepage from starks of coagulated rubber in the factory and effluents were collected from the four tanks used for washing the coagulated rubber. Three fungal species, Mucor racemous, Mucor sp. and Aspergilus niger, were isolated from the NRWS. From the effluents collected from the four wash tanks, five fungal species were isolated. Mucor recemous, Mucor sp., Aspergillus niger, Aspergillus, sp. and Rhizopus sp. Of all the species isolated, the Mucor species were found capable of utilizing NRWS as a growth substrate. Optimal growth of Mucor in NRWS was achieved at near-neutral pH of 7.1. It was also observed that as biomass of Mucor increased in NRWS, the BOD of NRWS decreased. Pollution strength of the wastes as determined by biochemical oxygen demand (BOD) and total solids were found to be highest with NRWS (440 and 4520) and wash tank (A) (370 and 3520) respectively.     

VA菌根菌与植物的抗病性

VA菌根菌（VAM菌）与植物土传病害的关系受到广泛的研究。本文从VAM菌对植物抗病性的影响及其可能性机理和相应的农业管理措施等方面进行了介绍和评述,同时提出问题和展望,并强调该方面的研究在可持续发展农业中的重要性。     

生活垃圾填埋场不同粒径陈垃圾中微生物的分布特征

用3种尺寸的方形筛筛分填埋龄为6～7a的陈垃圾,并对筛分得到的4种不同粒径垃圾中的微生物类群分布特征进行研究.研究结果表明,陈垃圾中含有非常丰富的微生物,可达到1012CFU·g-1,其数量分布随粒径的变化为:(《4mm)＞(8～25mm)＞(4～8mm)＞(＞25mm);兼氧细菌在各粒径陈垃圾微生物中占绝对优势,其可达1012CFU·g-1,其次为真菌和厌氧细菌,真菌最高可达107CFU·g-1,厌氧细菌最高可达106CFU·g-1,放线菌数量最少,但也能达105CFU·g-1;同类群的微生物在不同粒径垃圾中的分布不同,不同粒径垃圾中不同微生物类群的数量组成也不相同;兼氧细菌和真菌数量对数值与各项理化指标线性回归方程表明,2类微生物明显受到含水率、浸提氨态氮、BDM和pH的影响.     

白腐真菌反应器处理废水的研究进展

介绍了白腐真菌的生物学特性及其降解污染物的优势,综述了生物转盘反应器、搅拌釜生物反应器、气升式生物反应器、流化床生物反应器、填充床生物反应器、转管生物反应器、空心纤维反应器等白腐真菌反应器的结构特点和在废水处理方面的研究进展,指出了目前白腐真菌反应器应用中存在的问题。