斑马鱼基因组随机扩增DNA多态性研究

采用随机扩增DNA多态性（RAPD）技术，对毒性试验的模式生物斑马鱼（Danio rerio）基因组进行了分析。结果显示，在Amersham Pharmacia公司随机扩增引物系列中，分别在01号引物的829bp、534bp，02号引物的572bp，及06号引物的495pb，各出现1条稳定条带，而环磷酰胺作用后的斑马鱼基因组DAN随机扩增产物中，缺少了部分稳定条带。这可以明确判定受试斑马鱼的基因发生了改变，为水环境遗传毒性效尖的研究提供了初步的数据基础。     

湖泊水质参数空间分析中异常值的识别与处理

异常值的存在对湖泊水质参数局部估计精度产生重要影响 ,制约了地统计学在湖泊水质研究中的应用。文章以太湖为例 ,介绍了在湖泊水质参数研究中的三种异常值识别和处理的方法。应用这些方法可以有效地降低异常值的影响水平     

ECOSYSTEM DYNAMICS AND POLLUTION EFFECTS IN AN OZARK CAVE STREAM1

ABSTRACT: Subterranean ecosystems harbor globally rare fauna and important water resources, but ecological processes are poorly understood and are threatened by anthropogenic stresses. Ecosystem analyses were conducted from 1997 to 2000 in Cave Springs Cave, Arkansas, situated in a region of intensive land use, to determine the degree of habitat degradation and viability of endangered fauna. Organic matter budgeting quantified energy flux and documented the dominant input as dissolved organic matter and not gray bat guano (Myotis grisescens). Carbon/nitrogen stable isotope analyses described a trophic web of Ozark cavefish (Amblyopsis rosae) that primarily consumed cave isopods (Caecidotea stiladactyla), which in turn appeared to consume benthic matter originating from a complex mixture of soil, leaf litter, and anthropogenic wastes. Septic leachate, sewage sludge, and cow manure were suspected to augment the food web and were implicated in environmental degradation. Water, sediment, and animal tissue analyses detected excess nutrients, fecal bacteria, and toxic concentrations of metals. Community assemblage may have been altered: sensitive species‐grotto salamanders (Typhlotriton spelaeus) and stygobro‐mid amphipods—were not detected, while more resilient isopods flourished. Reduction of septic and agricultural waste inputs may be necessary to restore ecosystem dynamics in this cave ecosystem to its former undisturbed condition.     

长江铜陵段表层水中重金属含量及存在形态分布研究

通过测定长江铜陵段枯、丰水期江水中cu、Pb、Zn和cd不同形态的含量,分析了4种金属在江水中的存在形态分布,不同水期含量变化,水中悬浮物对金属吸附能力大小,以及近20年来含量的变化情况。结果表明,长江铜陵段江水中各重金属总量丰水期时大于枯水期,重金属各形态含量之间均有差异:丰水期时,各金属会被悬浮物以不同的方式携带进入水体中,cu、zn、Pb以活跃态和稳定态为主,Cd以活跃态为主;枯水期时,Zn主要以溶解态和稳定态为主,Pb以稳定态方式被携带,而80％的Cu、Cd是以溶解态形式存于水中。悬浮物(丰水期)对重金属的吸附能力大小顺序为Pb>Cu>Zn>Cd。与近20年江水中的重金属背景值比较,长江铜陵段重金属含量有普遍升高的趋势。     

ECONOMIC COSTS AND BENEFITS OF INSTREAM FLOW PROTECTION FOR ENDANGERED SPECIES IN AN INTERNATIONAL BASIN1

ABSTRACT: The U.S. Endangered Species Act (ESA) restricts federal agencies from carrying out actions that jeopardize the continued existence of any endangered species. The U.S. Supreme Court has emphasized that the language of the ESA and its amendments permits few exceptions to the requirement to give endangered species the highest priority. This paper estimates economic costs associated with one measure for increasing instream flows to meet critical habitat requirements of the endangered Rio Grande silvery minnow. Impacts are derived from an integrated regional model of the hydrology, economics, and institutions of the upper Rio Grande Basin in Colorado, New Mexico, Texas, and Mexico. One proposal for providing minimum streamflows to protect the silvery minnow from extinction would provide guaranteed year round streamflows of at least 50 cubic feet per second in the San Acacia reach of the upper Rio Grande. These added flows can be accomplished through reduced surface diversions by New Mexico water users in dry years when flows would otherwise be reduced below the critical level required by the minnow. Based on a 44‐year simulation of future inflows to the basin, we find that some agricultural users suffer damages, but New Mexico water users as a whole do not incur damages from a policy that reduces stream depletions sufficiently to provide habitat for the minnow. The same policy actually benefits downstream users, producing average annual benefits of over $200,000 per year for west Texas agriculture, and over $1 million for El Paso municipal and industrial water users, respectively. Economic impacts of instream flow deliveries for the minnow are highest in drought years.     

正交化方法在水质模型参数确定中的应用

本文根据常微分方程参数反问题的数学理论，将正交化方法同有限差分法结合用于确定水质模型参数，并与正则化方法、最速下降法和共轭梯度法作了比较。其计算结果对比表明，正交化方法具有快速、简便、可靠的特点。更适合于水质模型参数的确定。     

基于模糊评判人工神经网络的重大危险源辨识研究

国家已经制定了重大危险源辨识标准,辨识依据是危险物质的数量,主要适用于化工行业.本文对于国家重大危险源辨识标准不适合的行业,结合各行业发生事故的特点和工艺特征,综合利用模糊评判法和人工神经网络,辨识企业内存在的重大危险源.在某化工厂进行了检验,实践证明了方法的科学性和合理性.     

基于数据库的煤矿危险源辨识系统研究

目前,煤矿大多采用的是检查项和检查内容固定的安全检查表法,为提高煤矿危险源辨识效率和准确性,遏制煤矿事故的发生,从职业安全健康管理体系角度,探讨了煤矿危险源辨识问题,并设计了以煤矿安全信息管理系统为基础的危险源辨识系统,模拟煤矿现场实际情况;依据职业安全健康管理体系中危险源管理的模式筛选出数据库中的相关信息,动态生成与之相适应的安全检查表。同时对煤矿安全信息管理系统的结构、功能以及辨识程序进行了分析。     

煤矿安全预评价的未确知测度模型及应用

矿井自然灾害的安全预评价涉及不确定因素众多.本文根据未确知数学理论,建立了预评价指标体系、置信度识别准则及评分准则,构造了矿井安全预评价未确知测度模型,并对矿井的各个安全指标进行了定量分析.用上述方法对黄陵二号井自然灾害的评定结果与实际耦合较好,从而为煤矿建设项目的安全预评价开辟了一条新途径.     

陆上石油勘探开发企业危害辨识与风险控制浅析

本文针对陆上石油勘探开发企业作业过程中存在的主要风险,分析了各作业环节包括的主要危害因素,提出风险控制的方法,以确保有效控制石油勘探开发作业过程中的各类风险.