南京市4个污水处理厂的活性污泥中细菌的分离鉴定和抗生素耐药性分析

通过16S rDNA序列分析对南京CN、CE、JN和JM这4个污水处理厂的活性污泥中分离的细菌进行鉴定,采用Kirby-Bauer纸片琼脂扩散法分析细菌的抗生素耐药性,目的是阐明该地区污水处理厂活性污泥中细菌抗生素耐药性现状,探索污水及污泥的潜在环境风险.4个污水处理厂分别分离到7、9、8和11株菌落形态不同的细菌,上述35株细菌分属25个种,17个属.抗生素耐药性分析显示,97.1%的菌株具有抗生素耐药性,80%菌株具有多重耐药性.分离菌株对氨苄西林、卡那霉素、氯霉素、链霉素、庆大霉素、四环素、红霉素和大观霉素的耐药率分别为71.4%、37.1%、37.1%、57.1%、34.3%、68.6%、94.3%和65.7%.结果表明活性污泥中细菌耐药性严重;不同菌株间的耐药性分析显示,危害水产养殖业的病原菌气单胞菌具有严重的多重耐药性,所有芽孢杆菌对氯霉素、链霉素和庆大霉素敏感;污水处理厂应加强出水的消杀工作,避免二次污染.     

城市消防救援网络形态特征、抗毁性与鲁棒性研究*

为探讨城市消防规划系统救援能力的空间布局科学性,采用复杂网络理论构建某城市消防救援站现状与规划网络模型。首先从模型紧密度、性能效率、连通性等方面分析复杂网络基本网络特征;其次采用节点随机/蓄意攻击、边随机/蓄意攻击、混合随机/蓄意攻击研究网络结构的抗毁性;然后根据失效发生位置,探讨网络组织内部信息系统失活导致的合作中断的鲁棒性特征;最后针对不同的规划建设方案,讨论城市消防系统建设规划的中远期时序性。研究结果可为提高消防网络的救援能力提供理论支撑。     

阴极电极面积和膜面积对H型MFCs产电能力的影响

以生活污水为底物,碳棒作电极,构建H型瓶式微生物燃料电池(microbial fuel cells,MFCs),研究了阴极电极面积和膜面积对电池产电能力的影响.阳极以厌氧污泥作接种体,两室分隔物使用阳离子交换膜(cation exchange membrane,CEM),阳极不使用中介体,阴极使用无催化剂的普通碳电极....     

多孔吸声材料流阻测试实践介绍

本文简单介绍多孔吸声材料流阻测试的初步实践,以促进同行交流。     

添加剂对水泥固化体机械性能的影响

以蛭石、分子筛、玻璃纤维为添加剂进行水泥固化并进行抗压强度和抗冲击性测试,结果表明:在抗压强度方面,以玻璃纤维作为添加剂的固化体抗压强度最大,分子筛次之,蛭石最差。在抗冲击性方面,蛭石对水泥固化体的抗冲击性能没有改善作用;以分子筛为添加剂在一定程度上可以改善水泥固化体的抗冲击性;以玻璃纤维为添加剂的水泥固化体有很好的抗冲击性。     

Effectiveness and application of modified wind turbine coating: Adding ionic liquids to titanium dioxide and diatomaceous earth

Wind turbines are a green energy source that Taiwan has been endeavouring to develop in the past five years. Excellent onshore wind farms have already been developed; however, offshore development is inevitable because of Taiwan's limited land area. With regard to energy sustainability, operation and trouble-free maintenance of wind farms are essential. If damage to fan components can be mostly prevented, the operating cost and maintenance time would be considerably decreased; thus, remarkable economic benefits could be achieved. An appropriate coating can help a structure resist the stress of the working environment and improve its durability, resulting in lower maintenance cost and less staff being required to perform on-site repairs. Diatomaceous earth (DE) has been widely used to produce composite porous materials. In this study, epoxy resin and titanium dioxide were mixed with DE to increase the hydrophobicity of the surface of coatings and provide them photocatalytic self-cleaning ability. The ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate was also added to the coatings to prevent the corrosion of coated metallic material. This study employed thermal analysis to investigate the thermal stability of the coatings and the corrosion resistance of coated iron blocks. Finally, a self-made quartz container was connected to a volatile organic compound detector, and sun exposure was simulated using ultraviolet light. The results confirmed that the modified coating retained its functionality under the harsh working environment and can be applied to the surfaces of large machinery used in offshore wind turbine towers.     

Mitigating microbiological risks of potential pathogens carrying antibiotic resistance genes and virulence factors in receiving rivers: Benefits of wastewater treatment plant upgrade

● Abundance of MAGs carrying ARG-VF pairs unchanged in rivers after WWTP upgrade. ● Upgrade of WWTPs significantly reduced diversity of pathogenic genera in rivers. ● Upgrade of WWTPs reduced most VF (ARG) types carried by potential pathogens in rivers. ● Upgrade of WWTPs narrowed the pathogenic host ranges of ARGs and VFs in rivers. Wastewater treatment plants (WWTPs) with additional tertiary ultrafiltration membranes and ozonation treatment can improve water quality in receiving rivers. However, the impacts of WWTP upgrade (WWTP-UP) on pathogens carrying antibiotic resistance genes (ARGs) and virulence factors (VFs) in rivers remain poorly understood. In this study, ARGs, VFs, and their pathogenic hosts were investigated in three rivers impacted by large-scale WWTP-UP. A five-year sampling campaign covered the periods before and after WWTP-UP. Results showed that the abundance of total metagenome-assembled genomes (MAGs) containing both ARGs and VFs in receiving rivers did not decrease substantially after WWTP-UP, but the abundance of MAGs belonging to pathogenic genera that contain both ARGs and VFs (abbreviated as PAVs) declined markedly. Genome-resolved metagenomics further revealed that WWTP-UP not only reduced most types of VFs and ARGs in PAVs, but also effectively eliminated efflux pump and nutritional VFs carried by PAVs in receiving rivers. WWTP-UP narrowed the pathogenic host ranges of ARGs and VFs and mitigated the co-occurrence of ARGs and VFs in receiving rivers. These findings underline the importance of WWTP-UP for the alleviation of pathogens containing both ARGs and VFs in receiving rivers.     

矿井主风机不停风倒机系统最优控制研究

为解决矿井停机切换主要通风机过程中引起的井下风量波动很大,易造成瓦斯积聚等引发的安全问题,提出1种基于智能控制的主通风机稳风切换系统.建立动态风机倒机数学模型,提出基于改进粒子群算法优化的模糊自适应PID的控制方法,并应用MATLAB进行仿真实验,结果表明:控制方法控制效果明显,在系统运行的120 s内,井下风量波动始...     

Metabolic responses of indigenous bacteria in chicken faeces and maggots to multiple antibiotics via heavy water labeled single-cell Raman spectroscopy

The use of maggots derived from chicken faeces as fish diets might serve as a vehicle for the widespread of multiple antibiotic resistant bacteria(ARB) in the environment. Heavy water labeled single-cell Raman spectroscopy(D 2 O-Raman) was applied to detect the metabolic responses of indigenous bacteria in chicken faeces and maggots to different concentrations of combined colistin, kanamycin, and vancomycin. By incubating the samples with D 2 O and antibiotics, metabolically active bacterial cel...     

大气环境影响评价中典型气象日的确定及其应用

对某地区多年气象资料进行统计和分析,给出大气环境影响评价中典型气象日的确定方法。结合高架点源影响浓度的实例计算,可以方便地得到典型气象日条件下大气污染物的日平均浓度分布情况,能够更为真实地反映该地区的实际气象条件对大气污染物分布浓度的影响情况。