能源发展的资源环境约束条件分析

随着经济的高速增长,我国对能源的需求也将持续增长,能源短缺和环境污染的压力也会随之增加,而环境容量的过度使用,又会反过来约束能源的发展。在＂十二五＂期间确定我国的能源发展方向将成为我们重点研究的问题,对我国能源供应和消费现状进行调查研究,提出近几年来我国的能源生产总量持续小于能源消费总量,供需缺口呈现逐步上升的趋势。在此基础上,全面和系统的梳理环境质量、功能分区、总量控制和低碳经济等能源发展的环境约束条件,有助于实现能源的可持续发展,并且为进一步改革现有的能源环境政策提供参考。     

细菌生长与接种条件

通过对无菌室开紫外灯灭菌时间的长短进行空白营养琼脂培养基培养后细菌有无情况的比较分析,得出无菌室用紫外灯灭菌的最佳时间是30min;并通过接种时控制培养基温度为40～45℃,了解菌落分布均匀,生长良好的前提.     

Degradation of chlorpyrifos in laboratory soil and its impact on soil microbial functional diversity

Degradation of chlorpyrifos at different concentrations in soil and its impact on soil microbial functional diversity were investigated under laboratory conditions. The degradation half-lives of chlorpyrifos at levels of 4, 8, and 12 mg/kg in soil were calculated to be 14.3, 16.7, and 18.0 d, respectively. The Biolog study showed that average well color development (AWCD) in soils was significantly (P < 0.05) inhibited by chlorpyrifos within the first two weeks and thereafter recovered to the similar level as the control. A similar variation in the diversity indices (Simpson index 1/D and McIntosh index U) in chlorpyrifos-treated soils was observed, no significant difference in the Shannon-Wiener index H' was found in these soils. With increasing chlorpyrifos concentration, the half-lives of chlorpyrifos were significantly (P ≤ 0.05) extended and its inhibitory effects on soil microorganisms were aggravated. It is concluded that chlorpyrifos residues in soil had a temporary or short-term inhibitory effect on soil microbial functional diversity.     

寄生噬菌体和细菌宿主的本土适应性

<正>"生物多样性"很大程度上取决于外来物种在本土环境中的适应和发展.通常来说,当生存环境发生改变时,寄生生物能为其宿主调节生存竞争压力.已有大量证据表明寄生虫和宿主的确具有本土适应性,但是我们对地球上最为丰富的微生物体——细菌和他们的寄生噬菌体却知之甚少.实际上,微生物多样性最终决定了生态系统的功能和环境的变化.最近一系列的科学研究引发了科学界对微生物多样性的另一话题:那就是生物因素和非生物因素对促进生物多样性的相对重要性.     

Evaluation of the relationship between two different methods for enumeration fecal indicator bacteria： Colony-forming unit and most probable number

Most probable number (MPN) and colony-forming unit (CFU) estimates of fecal indicator bacteria (FIB) concentration are common measures of water quality in aquatic environments. Thus, FIB intensively monitored in Yeongsan Watershed in an attempt to compare two di erent methods and to develop a statistical model to convert from CFU to MPN estimates or vice versa. As a result, the significant di erence was found in the MPN and CFU estimates. The enumerated Escherichia coli concentrations in MPN are greater than those in CFU, except for the measurement in winter. Especially in fall, E. coli concentrations in MPN are one order of magnitude greater than that in CFU. Contrarily, enterococci bacteria in MPN are lower than those in CFU. However, in general, a strongly positive relationship are found between MPN and CFU estimates. Therefore, the statistical models were developed, and showed the reasonable converting FIB concentrations from CFU estimates to MPN estimates. We expect this study will provide preliminary information towards future research on whether di erent analysis methods may result in di erent water quality standard violation frequencies for the same water sample.     

氯对模拟管壁生物膜的氧化特性研究

以载片上培养的大肠杆菌生物膜为对象,研究了氯对模拟管壁生物膜中大肠杆菌的灭活效果,同时考察氯氧化生物膜后水中生物可同化有机碳(AOC)、生物可利用磷(MAP)和细菌生长潜能(BRP)的变化情况.结果表明,氯可以有效灭活悬浮态的大肠杆菌,而对生物膜中的大肠杆菌的灭活效率远低于悬浮菌;在相同CT值下,较高浓度的氯对悬浮态和生物膜中大肠杆菌的灭活效果要高于低浓度的氯.氯对生物膜的氧化作用会使生物膜中物质溶出,增加了水中AOC和MAP浓度,如当氯的浓度为1.0 mg/Ｌ(CT值为100 mg·min/L)时,水中AOC由20.78 μg/L增加到120.17 μg/L,MAP含量由0.11 μg/L增加到0.17 μg/L;氯的氧化作用会增加水的细菌生长潜能（BRP）,BRP随着CT值的增加而增加,如当氯投量为1.0 mg/Ｌ(CT值为100　mg·min/L)时,BRP可达到1.10×10⁷ CFU/mL.     

基于固定化氮循环细菌技术的镇江金山湖生态工程效果研究

通过细菌筛选、载体分析、固定化氮循环菌制备,获取了高效固定化氮循环细菌;采用固定化氮循环细菌技术对镇江金山湖富营养化水体进行了治理研究.结果表明,经过固定化氮循环细菌释放试验,金山湖示范工程区水体和水生植物根区氨化细菌、亚硝化细菌、硝化细菌和反硝化细菌数量明显增加,重建的金山湖示范工程区水体水质明显改善,TN、NH⁺₄-N、NO^-₃-N和NO^-₂-N平均比示范工程前分别降低44.70％、 67.17％、 31.79％和74.21％,氨氮达到Ⅱ类、总氮达到Ⅳ类水标准.固定化氮循环细菌技术对于荒漠化水体生态重建与修复具有重要意义.     

CSTR系统发酵产氢细菌群落动态与产氢能力关系解析

将CSTR系统内pH由4.2一次性提高至6.0左右,启动发酵类型的转化,研究了转化过程系统内的产氢动态和细菌群落.结果表明,在有机负荷维持在（33±1）kg/(m³·d)的情况下,发酵类型10 d内未发生改变,产氢量8 d内未降低,15 d后系统内种群由乙醇型转化为丁酸型,进水碱度由250 mg/L增至2 450　mg/L.研究中利用荧光原位杂交技术（FISH）对反应系统内3类微生物群进行监测发现,在转化过程中Clostridium cluster XI数量增加,Clostridium cluster Ⅰ 和Ⅱ数量减少,而Enterobacteriaceae始终存在,变化不明显.种群的消长同反应系统产氢能力的高低存在密切关联,以Clostridium cluster Ⅰ和Ⅱ占优势的乙醇型发酵具有更佳的产氢能力,平均比产氢速率为23.6 mol/(kg·d).     

我国环境功能区划框架体系的初步构想

环境功能区划的编制和实施将是实现我国环境保护分类指导、分区控制,并进一步优化国民经济发展格局的重要手段.本文分析了未来我国环境功能区划的需求;总结了现行区划研究基础与管理实践的问题;提出我国环境功能区划框架体系构想:在宏观层面采用综合引导区划,在区域层面上采用要素控制区划;最后探讨了这一框架体系下的技术难点和实践要点.     

盘锦市湿地资源利用与恢复生态功能探讨

分析了盘锦市湿地由于不合理的开发利用,导致生态功能受损状况;为防止湿地资源的过度开发,保护湿地生态系统的结构、物种、功能效应,使湿地资源永续利用,提出了具有可操作性的恢复措施。