Soil bacterial community characteristics of rice-rice-rape crop rotation北大核心CSCD

To investigate the characteristic changes in soil bacterial community under rice-rice-rape rotation in southern China, soil samples from a 30-year crop rotation and continuous cropping system were collected. Clone library and gene sequence analysis were adopted. The PCR amplification was carried out using universal primers of the 16S rDNA gene. The amplified fragments were then used to construct a clone library. The subclones were sequenced and analyzed. The experimental analysis showed that, in July 2015, and October and April 2016, the Shannon Wiener index and richness index of bacteria from the rotation treatment soils were higher than those of the continuous cropping treatment soil. The soil bacterial diversity of the rotation treatment was higher than that of the continuous cropping treatment. The results of BLAST analysis in the GenBank showed that Proteobacteria accounted 55% of total bacteria in the rotation treatment soil, whereas it accounted for 45% of total bacteria in continuous cropping treatment soil. Gemmatimonadetes accounted for 13% of the total bacteria in rotation treatment and 10% in the continuous cropping treatment. The proportion of Acidobacteria, Firmicutes, and Planctomycetes in rotation soil was less than those in the continuous cropping soil. Proteobacteria and Gemmatimonadetes were the dominant flora in soil. The changes in the predominant bacteria affected the diversity of soil bacteria in rotation and continuous cropping. The sequence analysis showed that the dominant bacteria in the soil were Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria, which higher than those in the rice-rice continuous cropping treatment. The sequences similar to Chloroflexi were observed in the rice-rice-rape rotation soils, but not in the continuous cropping soils. The results showed that the soil microbial population is conducted by agricultural tillage, and rice-rice-rape rotation increased the soil bacterial abundance compared with that of the rice-rice continuous treatment. © 2018 Science Press. All rights reserved.     

Tracking land cover change along the western edge of the U.S. Corn Belt from 1984 through 2016 using satellite sensor data: observed trends and contributing factors

The Cropland Data Layers (CDL) are high-resolution geo-referenced data products made available by the U.S. Department of Agriculture. However, the CDL lacks in its ability to be employed as a tool to identify the impact of the gradually evolving drivers of land use change, e.g., climate change, due to its limited historical depth. We implement a robust, phenology-based satellite image classification algorithm to identify historical cropland allocation in eastern South Dakota and North Dakota predating the initial CDL by 13 and 22 years, respectively. Five major land covers, i.e., corn, soybeans, wheat, alfalfa and grass (including native grass, hay and pasture) are identified using archived Landsat-5 surface reflectance data, while achieving CDL-like accuracy. The long-term rate of grassland loss during 1985–2011 is found to be significantly lower (26,781 hectares or 1.5% annually) relative to the near-term rate of grassland loss during 2006-’11 (84,545 hectares or 5.2% annually). We find similar discrepancy in regional corn expansion rates. Our value-added raster data provide opportunities for improved identification of land use drivers, whereas relying solely on the CDL’s restricted historical extent may lead to biased land use change estimates and misguide policy.     

A Cu-modified active carbon fiber significantly promoted H2S and PH3 simultaneous removal at a low reaction temperature

• Cu_0.15-ACF performs the best for H₂S and PH₃ simultaneous removal. • 550°C and 90°C are separately calcination and reaction temperatures. • The reason why Cu_0.15/ACF shows better performance was found. • The accumulation of H₂PO₄⁻ and SO₄²⁻(H₂O)₆ is the deactivation cause of Cu_0.15/ACF. Poisonous gases, such as H₂S and PH₃, produced by industrial production harm humans and damage the environment. In this study, H₂S and PH₃ were simultaneously removed at low temperature by modified activated carbon fiber (ACF) catalysts. We have considered the active metal type, content, precursor, calcination, and reaction temperature. Experimental results exhibited that ACF could best perform by loading 15% Cu from nitrate. The optimized calcination temperature and reaction temperature separately were 550°C and 90°C. Under these conditions, the most removal capacity could reach 69.7 mg/g and 132.1 mg/g, respectively. Characterization results showed that moderate calcination temperature (550°C) is suitable for the formation of the copper element on the surface of ACF, lower or higher temperature will generate more cuprous oxide. Although both can exhibit catalytic activity, the role of the copper element is significantly greater. Due to the exceptional dispersibility of copper (oxide), the ACF can still maintain the advantages of larger specific surface area and pore volume after loading copper, which is the main reason for better performance of related catalysts. Finally, increasing the copper loading amount can significantly increase the crystallinity and particle size of copper (oxide) on the ACF, thereby improving its catalytic performance. In situ IR found that the reason for the deactivation of the catalyst should be the accumulation of generated H₂PO₄⁻ and SO₄²⁻(H₂O)₆ which could poison the catalyst.     

活性污泥微生物群落结构及与环境因素响应关系分析

对已发表的62个来自国内外污废水处理设施中活性污泥的16S rRNA扩增子数据进行数据发掘和分析,旨在揭示不同进水性质、温度及处理工艺对应的污泥菌群结构特性.结果表明,活性污泥中整体有着较高的物种多样性和群落丰富度,不同活性污泥样品微生物群落结构有一定差异,样品中常见的优势菌属有Thauera、Nitrospira、Comamonas、Dechloromonas、Rhodoferax、Aquihabitans 和Acidovorax等.温度与Nitrospira、Aquihabitans、Terrimonas和Dechloromona等多种关键的脱氮微生物呈负相关,当温度低于15℃时,32座污水处理厂对TN和NH₄⁺-N的去除率仅有49.67%和63.19%.BOD₅/COD值越大的污水处理系统,Zoogloea、Arcobacter、Acidovorax和Acinetobacter等优势功能性菌属相对丰度也越高,污染物的去除率也越高.对于生活污水的处理,A²O工艺性能要优于OD、CAS和CMAS工艺,Comamonas、Rhodoferax、Nitrospira和Novosphingobium等多种功能性优势菌属的相对丰度要高于其它3类工艺.     

Trisomy 16 detected at chorion villus sampling

Trisomy 16 detected at chorion villus sampling (CVS) may reflect the placental but not the fetal karyotype. We describe a case in which the pregnancy continued until intrauterine death at 37 weeks. Cytogenetic study of two placental samples showed 47, +16 and 46,XX; the fetus was morphologically grossly normal, but fetal tissue culture was unsuccessful. Conservative management may be appropriate when trisomy 16 is detected at CVS and the pregnancy is normal ultransonographically.     

Prenatal diagnosis of 47,XX,der(15)t(15;16)(q13;p13.2)

A case of prenatally detected partial trisomy 15 and 16 is reported. Amniocentesis was performed at 14 weeks' gestation because a 6-mm nuchal translucency was detected on a dating ultrasound evaluation. Karyotype from amniocytes was suspect of an aberration concerning a marker chromosome. FISH analysis demonstrated that this marker chromosome was a der(15). A maternal chromosomal rearrangement t(15;16)(q13;p13.2) was confirmed. At birth, the proband was severely hydropic and had dysmorphic features, which included hypertelorism, micrognathia, incomplete separation of the maxilla and mandible, hyperflexed hands with overlapping fingers, hyposegmented right lung, and a single umbilical artery. Copyright © 2004 John Wiley & Sons, Ltd.     

基于“3S”技术构建农业灾害监测信息系统

本文从介绍遥感、地理信息系统、全球定位系统在建设农业灾害监测信息系统中的特点和优势出发,阐述了“3S”技术在农业灾害监测信息系统中的作用。在此基础上,提出了基于“3S”技术建设农业灾害监测信息系统的构想。     

计算机网络考试系统在山西煤矿安全培训中的应用实践

安全培训在煤矿安全管理中发挥着重要的作用。山西煤炭资源丰富,有包括地方、乡镇在内的煤矿4000余个,更需要通过科学、公正的考核手段保证安全培训质量,提高煤矿企业负责人的素质来保证煤矿的安全生产。传统的安全培训考试由于受到人力、物力和人情关系等主客观因素的影响,难以达到完全的公平、公正。而计算机网络考试通过题库随机出题、标准化判卷等技术手段恰好可以解决这些问题。由山西煤矿安全监察局组织开发的“山西省煤矿安全培训考试系统”,经过一年多安全培训考核的实践,取得良好的效果,基本实现了预期的设想。该系统的开发和应用为安全培训的科学化、规范化,为提高各类煤矿企业负责人的素质作出了有益的尝试。     

Structuring cooperative behavior under the National Environmental Policy Act of the United States

A trend is presently under way to streamline the compliance requirements of the National Environmental Policy Act of the United States through cooperative assessment procedures. Since the law was enacted in 1970, however, participants in the assessment process have tended to settle disagreements by adversarial rather than cooperative means. This study focuses on a highly acclaimed environmental assessment procedure undertaken by the U.S. Steel Corporation for its proposed $4.0 billion Lakefront Steel Plant. Survey research data were gathered to measure the underlying management philosophy perceived by interorganizational representatives, who attended a series of Technical Team meetings designed to coordinate the assessment effort and to open communication channels. A social psychological scale of behavioral dimensions was adapted from Likert to define the composition and the extent of cooperation at these meetings. A newly constructed scale was used to measure the quantity and quality of representation by each of the major organizations involved. Informal interviews were conducted with project participants to obtain further insight into member interaction. Results indicate that democratic problem solving was in evidence but also suggest a preference by respondents for improved procedures. The paper outlines areas of concern voiced by respondents and offers the means for improving the operating procedures of similar interorganizational environmental assessments.     

16S rRNA基因技术在环境科学领域中的应用

随着分子生物学的发展，16S rRNA基因技术被逐渐应用到环境科学领域中。目前在环境保护和治理中，该技术主要被用于鉴定污染物的生物降解菌和分析环境样品中的微生物群落多样性，由于它不必将微生物培养分离出来，也就避免了在培养过程中可能出现的微生物去失的情况。本文对16S rRNA基因技术及其在环境科学领域中的应用现状和发展作了一简要介绍，并对16S rRNA基因技术存在的不足进行了讨论。