Assessment of Population Dynamics of Specific Strains in Groundwater Bioaugmentation Experiments by Two Different Molecular Techniques

A set of microcosm experiments was performed to understand the behaviour of special degraders in bioaugmentation experiments. In the experiments the following chlorobenzene degraders were used: the genetically modified Pseudomonas putida F1CC, and the two wild-type strains Pseudomonas putida GJ31 and Pseudomonas aeruginosa RHO1. These strains were used at an initial cell density of 10⁵ cells mL^–1 groundwater which had been spiked with 1,2-dichlorobenzene (1,2-DCB), 1,4-dichlorobenzene (1,4-DCB) and, as main contaminant, chlorobenzene (CB). The population dynamics and behaviour of the three special degraders within the groundwater microcosms were studied by single-strand conformation polymorphism (SSCP) analysis of 16S rDNA fragments amplified from directly extracted community DNA and fluorescent in situ hybridization (FISH) with species-specific probes. RHO1 disappeared after 4 days as detected by FISH in contrast to SSCP-detection where RHO1 could be found during the whole incubation time. Whereas GEM F1CC and wild-type strain GJ31 survived the whole incubation for 20 days. With both methods we were able to detect all strains with high specificity among the indigenous microbial community. The data sets obtained from SSCP analysis and FISH were highly correlated. Specific band intensity within the SSCPfingerprints and the cell counts determined by FISH gave a quantitative overview about the introduced strains.     

Evaluation of theories and calculation tools in ecology

The concept of validation is inappropriate for the evaluation of ecological models. Classification of models into logical, theoretical, and calculation tool (predictive) types provides a sounder basis for choosing an evaluation technique. The dangers of model “tuning” are presented.     

Privacy-seeking behavior in an elementary classroom

This study observed privacy-seeking behavior in an elementary classroom, investigated individual differences in privacy seeking, and compared preferences for private spaces varying in degree of enclosure. Four privacy booths were placed in a fourth-grade classroom. A ticket system was used to assess booth use. Information on personality and background variables was obtained with self-report, peer, teacher, and parent questionnaires. After an initial period of enthusiasm, overall booth use declined sharply. However, analysis revealed substantial individual variation in booth use that remained consistent throughout the study. For boys, booth use was significantly correlated with teachers' ratings of sociability, aggressiveness, and distractibility. For girls, a significant positive relationship was found between privacy seeking at home and in school. Self-reported desire for privacy was uncorrelated with actual privacy-seeking behavior in the classroom. No significant differences in the use of the various booths were found, although self-reported preferences clearly favored the booth that allowed visual access to the rest of the classroom when desired.     

The prediction of scenic beauty from landscape content and composition

Two studies were conducted to examine distance classes of vegetation (foreground, middleground and background) and scene composition (presence of vegetation in left, center or right section of the image) as predictors of perceived scenic beauty. In study one, 41 students rated 63 landscape scenes with regard to scenic beauty. The Scenic Beauty Estimation Method was used to derive interval scale beauty values (SBEs). For each landscape image, areal measures of vegetation in each distance class and for each vertical section were taken and used as predictors. Presence of haze, clouds and human impacts were also recorded. Among the most important contributors to scenic beauty were amount of center middleground vegetation, and center background vegetation. Left foreground vegetation and right foreground vegetation were found to have significant and opposing regression weight signs—negative for the left and positive for the right. Study two was conducted to determine whether these opposing regression weight signs for foreground vegetation were due to a perceptual right-left bias or to some specific content in the image itself. In the second study, the photographic slides used to present landscape scenes to subjects were reversed so that the content which was previously on the right was now left, and vice versa. Thirty-nine students rated the reversed slides with regard to their scenic beauty. The signs of the regression weights in study two shifted such that left foreground was now positively valued and right foreground was negatively valued. This finding suggests that viewers are sensitive to foreground content and its placement in the image, and not simply to one side of the field of vision.     

National parks legislative mandate in the United States of America

Natural areas of national parks constitute unique ecological and aesthetic resources. Since the inception of national parks, it has been apparent that they are susceptible to threats that result in significant change and damage. Today, major issues concerning management of parks relate to questions of expanded visitor use and what types of facilities are to be deemed appropriate, that is, the dilemma of preservation versus use. In the United States of America, resolution of this dilemma must, strictly speaking, be based upon intent and meaning of Congressional legislation. The traditional interpretation of legislation in the U.S. has been to provide for a balance of use and preservation. However, extensive review of the literature of national park policy reveals a remarkable lack of elaboration and clarification of legislative meaning. Based upon a critical analysis of pertinent legislation, which has heretofore been ignored, we provide an interpretation of legislative meaning which strongly supports a policy of preservation of park resources.     

Analysis and application of forest fuels data

Recent advances in fire modeling permit quantitative estimations of fire behavior from quantitative inputs that describe the fuel array and conditions, such as weather and site data, under which it will burn. This paper describes the collection, analysis, and stratification of flammable forest fuels data for coniferous forest ecosystems in Montana and then illustrates the resource management application of these data in three areas: the development of the fire behavior model, a determination of the model's sensitivity to input errors as reflected by fire behavior prediction errors, and the development of a fire hazard simulator (TAROT). A new integrated stand simulator, GANDALF, is highlighted.Conclusions center on the need to integrate fire management into the land management planning decision-making process.This work was supported by a USDI National Park Service contract to Gradient Modeling, Inc., a nonprofit research foundation devoted to ecologic research and resource management applications, and by cooperative aid agreements between Gradient Modeling, Inc. and the USDA Forest Service, Intermountain Forest and Range Experiment Station, Northern Forest Fire Laboratory (Fire in Multiple Use Management, R, D, and A Program).     

Simulated grazing responses on the proposed prairies National Park

The tallgrass prairie version of the ELM Grassland Model was used to evaluate the potential impact of establishing a tallgrass prairie National Park in the Flint Hills region of Kansas. This total ecosystem model simulates (a) the flow of water, heat, nitrogen, and phosphorus through the ecosystem and(b) the biomass dynamics of plants and consumers. It was specifically developed to study the effects of levels and types of herbivory, climatic variation, and fertilization upon grassland ecosystems. The model was used to simulate the impact of building up herds of bison, elk, antelope, and wolves on a tallgrass prairie. The results show that the grazing levels in the park should not be decreased below the prepark grazing levels (moderate grazing with cattle) and that the final grazing levels in the park could be maintained at a slightly higher level than the prepark grazing levels.     

污泥干化芦苇床中芦苇内生菌群多样性研究

以污泥干化芦苇床中芦苇内生菌的多样性作为主要研究对象,结合16S rDNA高通量测序技术与PCR-DGGE技术,对污泥干化芦苇床和天然湿地中污泥菌群及芦苇内生菌群多样性进行了比较分析。结果表明,污泥干化芦苇床芦苇内生菌的细菌类群包含了20门、30纲、73目、152科、467属、1 167种细菌。其中变形菌门(65.6%)和拟杆菌门(25.5%)占绝对优势,γ-变形菌纲是丰度最高的纲,占总数的43.4%;排前20位的优势菌种相对百分比之和为46.5%,分属于所对应的18个属、12科、10目、6纲、3门。DGGE分析表明,污泥干化芦苇床中的污泥和芦苇内生菌优势菌群的种类与天然湿地相比差异显著;同时由于污泥的影响,芦苇内生菌群种类也发生了较为明显的变化。     

基于.NET的B(S)/结构的环境管理信息系统的设计和开发研究

针对某企业目前环境管理的实际情况,分析该企业环境管理的各项需求,在对环境管理的的系统架构和功能平台进行充分设计的基础上,开发出一套较为完整的企业环境管理信息系统,使得企业中各部门能快捷共享环境数据库,提高企业处理环境事务的信息化水平.在系统的实现上,选定ASP.Net作为WEB服务端的开发技术,SQL Server作为后台数据库技术,完成基于.NET的B/S结构的企业环境管理信息系统的设计和开发,并成功应用于该企业的环境管理,满足了对环境管理信息化的需求.