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41.
ROH Mark S DE BENEDETTI Laura JOUNG Young Hee LEE Nam Sook 《应用与环境生物学报》2007,13(2):145-151
Germplasm collection is important to preserve and maximize genetic diversity for germplasm conservation. Tsuga dumosa ( D. Don) Eichler in Engler & Prantl. and T. chinensis var. forrestii (Downie) Silba germplasm was collected from three localities in China: Mt. Yulong, Wenfeng Temple and Mt. Dishiergu, Yunnan Province. Accessions were identified based on morphological characters and RAPD markers. The shapes of the apices and margins of needles were examined, and the length and width of needles, cones and seeds from accessions of mature plants were used to compare the morphological differences and to identify the germplasm. Molecular markers generated by randomly amplified polymorphic DNA (RAPD) were also used to characterize the taxa. Although the clustering based on RAPD markers was inconsistent with the morphological characters of the needles, based on the overall morphological characters and on RAPD markers, the accessions from Mt. Yulong and Wenfeng Temple were identified as T. chinertsis var. forrestii, and those from Mt. Dishiergu identified as T. dumosa. Taxonomic identification of the accessions was made based on morphology and by RAPD markers concurred. The results indicate that the shapes of the apices and margins of needles particularly from young plants could not be used as a possible key to identify T. dumosa and T. chinertsis var. forrestii. Fig 6, Tab 3, Ref24 相似文献
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含油废水处理过程中优势菌群的筛选 总被引:7,自引:0,他引:7
研究了用生物法处理含油废水的微生物净化效能,对整个处理装置的各反应池中的降解菌群进行了筛选。共分离出45株菌,分别属于14个属。并测定出芽孢杆菌在曝气池、二沉法和排水口三处占优势。 相似文献
44.
光合细菌净水剂清除滇池草海蓝藻的试验研究 总被引:5,自引:0,他引:5
光合细菌净水剂对也草海蓝藻清除有较好效果,在滇池草海约800m^3围栏水域的现场试验中,叶绿素-a去除率在75%以上,藻量去除率在90%以上,平均透明度从0.3m增加到1.06m,基本上已达到试验验期目标,且有操作简便,无二次污染产物等特点。 相似文献
45.
Pierre-Yves Ancion Gavin Lear 《Environmental pollution (Barking, Essex : 1987)》2010,158(8):2738-2745
We investigated the absorption rates of zinc, copper and lead in freshwater biofilm and assessed whether biofilm bacterial populations are affected by exposure to environmentally relevant concentrations of these metals in flow chamber microcosms. Metals were rapidly accumulated by the biofilm and then retained for at least 14 days after transfer to uncontaminated water. Changes in bacterial populations were assessed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 16S rRNA gene clone libraries. Significant differences in bacterial community structure occurred within only three days of exposure to metals and remained detectable at least 14 days after transfer to uncontaminated water. The rapid uptake of stormwater-associated metals and their retention in the biofilm highlight the potential role of biofilms in the transfer of metals to organisms at higher trophic levels. The sensitivity of stream biofilm bacterial populations to metal exposure supports their use as an indicator of stream ecological health. 相似文献
46.
Diversity of chlorobiphenyl-metabolizing bacteria and their biphenyl dioxygenases in contaminated sediment 总被引:1,自引:0,他引:1
Bacteria and bacterial communities in sites contaminated with polychlorinated biphenyls have been extensively studied in the past decades. However, there are still major gaps in the knowledge of environmental processes, especially in the behavior of previously described bacteria in vitro, their real degradation abilities and the enzymes that are involved in the degradation processes. 相似文献
47.
从哈尔滨制药总厂的青霉素发酵药渣中富集筛选出6株青霉素耐受菌株,经驯化后,采用高效液相色谱法检验其对青霉素的降解效率,经比较得到一株青霉素高效降解菌株,编号为JZ6。青霉素浓度300 mg/L,30℃,p H6.7,121 r/min条件下恒温震荡培养24 h后,在青霉素做唯一碳源情况下,青霉素几乎不被降解,外加碳氮源后降解率可达到99.98%。对其进行电镜扫描和16SrDNA序列分析,鉴定其属于洋葱伯克霍尔德菌属(Burkholderia cenocepacia),16SrDNA序列长度为1451bp,在Gen Bank登录号为KF826288。 相似文献
48.
溶藻细菌L7代谢物对水华鱼腥藻的溶藻效应 总被引:2,自引:1,他引:1
从等体积不同密度的溶藻细菌L7菌液中提取胞内、胞外代谢物,分别加入等体积等密度的水华鱼腥藻中连续培养7d,研究L7代谢物对水华鱼腥藻的溶藻效应。2.78×105~2.78×10 7cfu/mL的L7胞内代谢物能显著促进藻生长和异形胞分化,到培养结束藻密度为对照组的1.7~1.98倍;异形胞数最大可为对照组的2.31倍。2.78×10 8、2.78×10 9cfu/mL的L7胞内代谢物能抑制藻生长,藻密度最低为对照组的58%;能极显著抑制异形胞分化,可不形成异形胞。2.78×10 5~2.78×109 cfu/mL的L7胞外代谢物能极显著抑制藻生长和异形胞分化,藻密度最低为对照组的0.63%,不形成异形胞;藻丝的藻细胞间隔变模糊,藻细胞变大变形,并有大量的单个球形藻体散落。L7主要通过分泌胞外代谢物溶藻,菌胞内代谢物对藻生长有"低促高抑"的作用。 相似文献
49.
介绍我国危险废物鉴别体系的组成,分析了2007版《危险废物鉴别标准》的相关内容,探讨了在危险废物鉴别实验室能力建设中,场地条件、硬件设备、人员技能3方面的要求。指出目前环境监测部门开展危险废物鉴别存在的问题和难点,有针对性地提出建议与措施。 相似文献
50.