单细胞凝胶电泳技术在环境污染物检测中的应用

主要介绍了单细胞凝胶电泳技术(SCGE),包括SCGE的原理、方法及其在环境污染物检测中的应用,该技术可用多种污染物的检测且灵敏度高,具有广阔的应用前景。即SCGE对大气污染物、重金属、醛类及辐射污染的检测,具有高度敏感性,是具有广阔应用前景的新技术。     

喷射浮选处理含油污水的研究

重点对喷射浮选处理含油污水进行了试验研究，结果表明浮选室内射流泵的没水深度对喷射选除油效果有较大的影响，并且只有在气泡量大并充满浮选室及表面相对平静的状态下，才儿得较好效果，为此，我们在浮选室内加入自制的增料，使兼并的气海重新得到分散，消耗了一部分动能，减缓了浮选室的扰动，使除在大提高，从而为研制新型高效的喷射浮选设备提供了一条新途径。     

Reduction of sera requirements in amniotic fluid cell culture

Reduction in serum requirement for culture of primary human amniotic fluid cells can be achieved by the addition of 10 growth-promoting factors to the nutrient medium. This supplemented medium preserves cell types normally found in amniotic fluid cell cultures supplemented with 20–30 per cent fetal bovine serum. The volume of amniotic fluid required to initiate culture can be as little as 1 ml. Amniotic fluid samples contaminated with red blood cells with no visible clot also grow well in the low serum medium. Cell-free amniotic fluid combined with equal parts of supplemented medium is useful in initiating cell culture.     

The effect of oxygen tension on colony formation and cell proliferation of amniotic fluid cellsin vitro

The effects of reduced oxygen concentrations in the gas phase over the culture medium on colony formation and cell proliferation were investigated in high and low cell density primary and secondary cultures of amniotic fluid cells. Using two standard culture methods (25 cm² plastic flasks and Leighton type tubes) a significantly reduced culture time was observed at high cell density for mass cultures by incubation within a low oxygen tension gas phase (2.5 per cent to 7.5 per cent O₂) instead of conventional air (18 per cent O₂). At low cell density colony formation was significantly enhanced in cultures grown at reduced oxygen tension. Using gas permeable membranes as support, lowering the oxygen tension from 7.5 per cent to 2.5 per cent yielded an increase in plating efficiency of cells from approximately 5 per cent to 25 per cent, whereas plating efficiency was less than 2 per cent for cells grown at ambient 18 per cent O₂. It is suggested that low oxygen tension in the gas phase is an effective means of enhancing clonal growth in amniotic fluid cell cultures, thereby reducing both culture time and risk of culture failure.     

污染源废气中氟的监测方法研究

本文叙述了有组织废气中氟化物的测定方法。该法用碱性滤膜捕集氟化物，使现场操作大为简化，并使精密度和准确度都比溶液吸收法有较大改善。本文给出了方法的研究结果，最后给出方法的操作程序     

基于TMS320F240 DSP的电网谐波抑制全数字化控制的研究

为治理电力系统谐波污染 ,提高电力系统运行的安全稳定性 ,笔者对混合式并联有源电力滤波系统的结构和工作原理进行了分析和研究。该系统由无源滤波器和小容量有源滤波器串联构成 ,与被补偿的谐波负载并联连接。基于TMS32 0F2 4 0DSP开发套件 ,设计数字控制器主程序和中断服务子程序。实验结果表明 ,采用TMS32 0F2 4 0DSP编程方便并直观 ,运算精度高 ,控制器参数调整方便 ,易于实现各种控制策略。研究成果对DSP在有源电力滤波系统中的应用有重要参考价值。     

固定化酵母菌对活性艳蓝KN-R的脱色研究

采用海藻酸钙加活性碳的方法对酵母菌进行固定化。正交试验结果表明，三者合适配比为海藻酸钠3％，活性碳10％，氯化钙4％。此方法制得的凝胶颗粒机械强度好、经久耐用。固定化酵母菌凝胶小球最适条件为：培养时间为48h，培养温度为37℃，培养基pH为3．0，凝胶量为10g。固定化细胞经多次脱色后能重复使用，4次脱色后其脱色率还达79．2％。     

滤膜法测定空气中氟化物的改进

测定环境空气中微量氟的方法一般采用滤膜法，但此法工作量大，针对这种情况，本文作两点改进：一是将采集的两张滤膜同时测定；二是将测定过程中的搅拌时间缩短，这样不影响测定结果的准确性，而且降低了工作量。     

过滤捕集法处理含DEP雾废气的研究

为了探讨玻璃纤维过滤器处理含DEP雾废气的效率问题,在实验室进行了DEP雾去除实验。考查了不同气体入口浓度、过滤流速下玻璃纤维过滤捕集装置对含DEP雾废气的净化能力,结果表明:利用玻璃纤维过滤器去除DEP雾,在DEP雾粒径主要分布为0.48～3.4μm之间,DEP雾浓度小于220 mg/m3,过滤流速小于10 cm/s情况下,玻璃纤维过滤器对DEP雾的去除率大于99%,最大气体流量下的压力降小于1610Pa;同时对含油(酸)雾废气除雾效果好,特别是对于粒径特别细微的油(酸)雾,捕集效率也很高,还可回收利用,不会产生二次污染。     

干式烟气脱硫对除尘器性能影响的分析

简单介绍了喷雾干燥法，炉内喷钙法，循环流化床脱硫法的工艺流程，讨论了这3种脱硫系统运行后除尘器入口处烟尘浓度、烟气温度、湿度和粉尘比电阻、粒径、粘性等性质的变化，以及对静电除尘器和布袋除尘器的不同影响。