深海热液喷口周围微氧耐压细菌的培养研究

研究采用析因实验设计,探讨了培养基的水源、培养温度和生长pH等因素对喷口细菌生长繁殖的影响,初步探索了常压下东太平洋某海底喷口周围微氧耐压细菌的生长条件。样品培养10d后,对培养液中细菌量进行显微计数,实验数据用SPSS11.0统计软件中的方差分析程序进行处理。统计结果显示:在标准大气压下,从研究样品中获得最多细菌数的较优培养条件为:培养基水源为单蒸水;生长pH为7.60;培养温度为50℃。在该条件的培养液中细菌平均浓度达2.596×10个/mL。研究结果为进一步认识、鉴定与开发利用深海喷口周围细菌资源打下了基础。     

混合型微生物絮凝剂产生菌的最佳培养条件

从活性污泥中筛选出具有较高絮凝活性的混合菌MBFH,研究了各种条件对混合菌产絮凝剂的影响.结果表明,混合菌MBFH产絮凝剂的最佳培养条件为:温度30℃、初始pH8.0、摇床转速为120r/min、培养基含糖量2%、相对接种量15%,其对高岭土悬浊液的絮凝率可达到88%.图5表4参9     

PP333在库拉索芦荟组培快繁中的应用研究

以MS 6-BA2.5mg/L NAA0．2mg/L为初代培养基，以MS 6-BA2.0mg/L NAA0．1mg/L为增殖培养基，1，3MS IBA0.5mg/L为生根培养基，添加不同浓度的PP333，其结果表明：初代培养基中添加0．5mg/LPP333有利于库拉索芦荟外植体出芽，增殖培养基中添加0．1-0．5mg/LPP333对库拉索芦荟试管苗增殖有效，而在生根培养基中添加0．5—1．0mg／LPP333有利于生根和移栽，其中以1／3MS IBA0．5mg/L PP3330．5mg/L培养基对库拉索芦荟试管苗生根和移栽有良好效应．     

Highly efficient and selective removal of phosphate from wastewater of sea cucumber aquaculture for microalgae culture using a new adsorption-membrane separation-coordinated strategy

● A new adsorption-membrane separation strategy is used for phosphate removal. ● PVC/Zr-BT shows a selective adsorption ability to low-concentration phosphate. ● Low concentration of P below 0.05 mg/L was achieved in actual wastewater treatment. ● Algal biomass production served as a demonstration of phosphorus recycling. Enhanced phosphorus treatment and recovery has been continuously pursued due to the stringent wastewater discharge regulations and a phosphate supply shortage. Here, a new adsorption-membrane separation strategy was developed for rational reutilization of phosphate from sea cucumber aquaculture wastewater using a Zr-modified-bentonite filled polyvinyl chloride membrane. The as-obtained polyvinyl chloride/Zr-modified-bentonite membrane was highly permeability (940 L/(m²·h)), 1–2 times higher than those reported in other studies, and its adsorption capacity was high (20.6 mg/g) when the phosphate concentration in water was low (5 mg/L). It remained stable under various conditions, such as different pH, initial phosphate concentrations, and the presence of different ions after 24 h of adsorption in a cross-flow filtration system. The total phosphorus and phosphate removal rate reached 91.5% and 95.9%, respectively, after the membrane was used to treat sea cucumber aquaculture wastewater for 24 h and no other water quality parameters had been changed. After the purification process, the utilization of the membrane as a new source of phosphorus in the phosphorus-free f/2 medium experiments indicated the high cultivability of economic microalgae Phaeodactylum tricornutum FACHB-863 and 1.2 times more chlorophyll a was present than in f/2 medium. The biomass and lipid content of the microalgae in the two different media were similar. The innovative polyvinyl chloride/Zr-modified-bentonite membrane used for phosphorus removal and recovery is an important instrument to establish the groundwork for both the treatment of low concentration phosphate from wastewater as well as the reuse of enriched phosphorus in required fields.     

花卉无土栽培技术的研究(Ⅰ)

花卉无土栽培是在土壤栽培的基础上发展起来的现代农业高新技术,本文作者经多年来的无土栽培试验,根据当地条件,改良了霍格兰和阿农(Hogagland&Arnon)配方,改良配方效果显示:无土栽培的花卉其叶色浓艳,叶绿素含量较有土栽培一般高10~20%;黄枯叶较土壤栽培少60~80%左右,耗水量、病虫害较土壤栽培分别少40~60%、75~100﹪,且无杂草,可观期延长.     

用好防灾科技学院教育平台 创建和弘扬防震减灾文化——恭贺母校升本暨校庆30周年

本文对防震减灾文化的组成、创建动力及其方法进行了探讨。初次提出了防震减灾文化的概念，并对创建防震减灾文化的目的和如何创建、弘扬防震减灾文化提出了看法和观点。     

培养藻类制取生物能源的研究

能源紧张和环境污染是全世界所面临的共同问题,用藻类替代传统作物生产生物能源是生物质能的重要发展方向之一。文章介绍了国内外对藻类培养条件、光生物反应器型式、藻类基因工程、藻细胞收集方法以及能源藻类生产系统的研究成果。研究认为,以藻类为原料生产生物燃料具有良好的发展前景,并指出了今后能源藻类生长的重点研究方向。     

经济战略研究与可持续发展的若干思考

本文分析了战略研究演化的轨迹,揭示了可持续发展的深刻内涵,指出了21世纪我国可持续发展面临的机遇。     

Effect of Cell-to-matrix Ratio in Polyvinyl Alcohol Immobilized Pure and Mixed Cultures on Atrazine Degradation

Atrazine biodegradation by immobilized pure and mixed cultures was examined. A pure atrazine-degrading culture, Agrobacterium radiobacter J14a (J14a), and a mixed culture (MC), isolated from an atrazine-contaminated crop field, were immobilized using phosphorylated-polyvinyl alcohol (PPVA). An existing cell immobilization procedure was modified to enhance PPVA matrix stability. The results showed that the matrices remained mechanically and chemically stable after shaking with glass beads over 15 days under various salt solutions and pH values. The immobilization process had a slight effect on cell viability. With the aid of scanning electron microscopy, a suitable microstructure of PPVA matrices for cell entrapment was observed. There were two porous layers of spherical gel matrices, the outside having an encapsulation property and the inside containing numerous pores for bacteria to occupy. J14a and MC were immobilized at three cell-to-matrix ratios of 3.5, 6.7, and 20 mg dry cells/mL matrix. The atrazine biodegradation tests were conducted in an aerobic batch system, which was inoculated with cells at 2,000 mg/L. The tests were also conducted using free (non-immobilized) J14a and MC for comparative purpose. The cell-to-matrix ratio of 3.5 mg/mL provided the highest atrazine removal efficiency of 40–50% in 120 h for both J14a and MC. The free cell systems, for both cultures, presented much lower atrazine removal efficiencies compared to the immobilized cell systems at the same level of inoculation.     

Enhanced production of poly(3-hydroxybutyrate) by filamentation-suppressed recombinantEscherichia coli in a defined medium

Fed-batch cultures of recombinantEscherichia coli strains were carried out for the production of poly(3-hydroxybutyric acid) (PHB) in a chemically defined medium. TheE. coli strains used were XL1-Blue, harboring pSYL105, a stable high-copy number plasmid containing theAlcaligenes eutrophus polyhydroxyalkanoate (PHA) genes, and XL1-Blue, harboring pSYL107, which is pSYL105 containing theE. coli ftsZ gene to suppress filamentation. With XL1-Blue(pSYL105) the final cell mass and PHB concentration obtained in 62 h were 102 and 22.5 g/L, respectively. Fed-batch culture of XL1-Blue(pSYL107) under identical conditions resulted in a final cell mass and PHB concentration of 127.5 and 48.2 g/L, respectively. The PHB contents obtained with XL1-Blue(pSYL105) and XL1-Blue(pSYL107) were 22.1 and 37.8%, respectively. Therefore, PHB was more efficiently produced in a defined medium by employing filamentation-suppressed recombinantE. coli.