Development of enzyme-linked immunosorbent assays for plasma vitellogenin in Chinese rare minnow (Gobiocypris rarus)

Due to the wide occurrence of endocrine disrupting chemicals in the environment, it is much of importance to develop high throughput screening method for the analysis of this kind of pollutants. Using anion-exchange membrane chromatography, vitellogenin (VTG) from the plasma of 17β-estradiol (E₂) treated Chinese rare minnow was rapidly purified within 15 min. Both polyclonal antibody (PcAb) and monoclonal antibody (McAb) against rare minnow VTG (R-VTG) were prepared in rabbit and Balb/c mice, respectively. The competitive enzyme-linked immunosorbent assays (ELISA) based on either PcAb or McAb were developed to identify and quantify R-VTG in the plasma, and these two methods showed similar characteristics. The detection limits of both assays were lower than 3 ng mL⁻¹ with the working ranges covering three magnitudes. The recovery efficiencies of PcAb and McAb based ELISA were 104.2% and 102.6%, respectively; and the intra-assay and inter-assay of these two assays were 6.2% and 9.2%, 8.6% and 12.8%, respectively. These results indicated that the described competitive ELISA methods were sensitive and valuable tools for quantifying vitellogenin in rare minnow plasma. These methods were then applied to measure R-VTG concentrations in plasma of male fish exposed to a series of E₂ concentrations. When E₂ levels were less than 10 ng L⁻¹, R-VTG levels in plasma were comparable to that in solvent control, while R-VTG levels significantly increased 15-folds and 350-folds, respectively when E₂ exposure concentrations were controlled at 10 and 50 ng L⁻¹. The high sensitivity of Chinese rare minnow to E₂ was demonstrated, making it a valuable model species to study environmental estrogens.     

Analysis of pesticide residues using the Quick Easy Cheap Effective Rugged and Safe (QuEChERS) pesticide multiresidue method in traditional Chinese medicine by gas chromatography with electron capture detection

The Quick Easy Cheap Effective Rugged and Safe multiresidue method (QuEChERS) has been validated for the extraction of 15 organochlorine pesticides (OCPs) in completely different matrices of traditional Chinese medicine (TCM). The method employed a rapid, simple and cost-effective procedure. The spiking levels for the recovery experiments were 0.1, 0.5 and 2.0 mg kg⁻¹. Mean recoveries mostly ranged between 76.0% and 114.0% (96.0% on average), and relative standard deviations (RSD) were generally below 10% (4.72% on average). Based on these results, the methodology has been proven to be highly efficient and robust and thus suitable for monitoring the MRL compliance of a wide range of commodity. The contamination status of 15 OCPs on 80 different TCMs marketed in China, and a total of 400 samples were also performed using a previously validated method. 15 OCPs were benzene hexachloride (BHC, including α-BHC, β-BHC, γ-BHC, δ-BHC), hexachlorobenzene, heptachlor, heptachlor epoxide, aldrin, p, p′-DDE, p, p′-DDD, o, p′-DDT, p, p′-DDT, mirex, endrin, dieldrin. This study indicates that the proposed method is useful for analyzing OCPs in TCM.     

环境规制对技术效率和生产力损失的影响分析

以中国工业为研究对象,以经济发展程度不同所分的东部、中部和西部地区兰个地区作为研究区域,采用非参数数据包络法(DEA)中的径自效率测量方法(radial efficiency measure)为主要研究方法,分析了在1998-2005年期间环境规制对中国工业的技术效率和生产力损失的影响.研究结果表明,在指定的研究期间,环境规制使得中国工业技术效率提高,但是对于生产力的发展却产生了负面的影响.三个地区之间受环境规制影响的差异较大,其中东部为环境规制所付出的成本最大,即东部为环境污染所付出的环境成本最高,这也是为何从九十年代开始,污染物从东部往中西部转移的原因;从受污染物影响的角度来看,因控制废水而引起的生产力损失大于因控制SO2而引起的生产力损失,即对于中国工业,废水的环境成本大于SO2的环境成本.另外,从研究结果中也可知,在中国现有的生产方式下,实施严格的环境规制有一定难度,所以改变旧的生产方式,实施可持续发展的生产方式是当务之急.     

中华鳖出血性肠炎的药物防治研究

对由实验定筛选的中华鳖出血肠炎病病原菌的最佳防治药物为本正交试验的第6组,即A2B3C1,其抑菌圈达21.5mm.临床应用中采用中西结合复方制剂,即用第6组试验药物再加上中药白头翁汤(加减),其疗效更佳.     

中草药饲料添加剂的开发应用

中草药饲料添加剂具有较化学药物添加剂十分明显的优势,尤其在食品安全方面的绝对优势使其在畜牧业上地位日显重要.本文介绍了中草药饲料添加剂的类别、特点、机理及目前应用的情况.     

中药生产中的综合利用途径

综合利用中药资源,开展中药生产中的废料综合利用工作,是保证中药资源永续利用的重要措施和手段。本文概述了中药生产中的综合利用及研究情况,探讨了中药生产中废料资源化技术的开发。     

四川中药资源的开发利用与发展方向

本文探讨了四川中药资源的开发利用和发展方向；（１）以资源化优势为依托，大力发展创泡中草药；（２）以地道药材为优势，建立稳定的药材基地（３）以综合利用为途径，提高资源利用的数量和质量；（４）以资源保护为前景，保证资源的永续利用。本文     

水资源供需平衡的安全分析

从中国发展的区域基础角度,考察经济核心区和6个大区在未来经济发展中的水资源供需安全问题.在分析中应用一般均衡的分析技术和线性规划逆问题分析技术相结合,研究表明,在未来发展中水资源最紧缺的地区是华北和西北,作为经济核心区的京津唐地区水资源问题最为严重.研究提出,只有结合开源节流和调整产业结构才能保证经济发展中的水资源供应安全.     

构造运动与中国沿岸平原的地质灾害

中国海岸穿过构造沉降带和隆起带，沉降带及隆起带断陷盆地的持续下降吸引了源远流长的大河在此人海，而隆起带内入海的河流多为近源小河，从而引起河流泥沙沿岸分配的不均和海岸沉积物的差异。沉降带内每公里海岸线接受的沉积物是隆起带的数十倍，且以砂质沉积物为主．隆起带河流泥沙来量少．而且砂质沉积物占了压倒的优势．河流泥沙沿岸分配的不均决定了中国沙质和淤泥质海岸的基本类型和分布格局、沉降带和隆起带松散沉积层的厚度和结构，山此产生了中国沿岸与第四纪沉积层有关的灾害和环境问题的有规律的分布。地面沉降主要分布于沉降带内，而地下海水入侵多出现在依赖地下水的隆起带内，底辟构造、浅层天然气往往在大河三角洲构成灾害和潜在的地质灾害．沉降带和隆起带断陷盆地内的海岸低地通常是海平面上升的主要脆弱地区．此外，丰富的河流泥沙使中国海岸对全球海平面上升的响应也不相同，如海岸湿地的再生和海岸侵蚀原因的复杂性等．