野猪和几种家猪亲缘关系的RAPD分析

对二花脸猪、红灯笼猪、姜曲海猪、香猪、约克夏猪及华东地区野猪共２４个个体进行了随机扩增多态ＤＮＡ（ＲＡＰＤ）分析,以探讨家猪与野猪间的亲缘关系．实验从４０个引物中选用重复性较好的１３个引物对所有个体进行了随机扩增,共获得１９２个ＲＡＰＤ标记．ＲＡＰＤ研究结果表明：（１）长江下游江苏地区家猪品种或类群内遗传变异相对较小,群体的遗传趋异程度处于较低水平;（２）二花脸猪、红灯笼猪及姜曲海猪与华东地区的野猪可能起源于一个共同的祖先;（３）中国家猪各地方品种可能由分布于各地区的亚洲野猪经人们长期的定向选育而形成     

Seasonal and spatial occurrence and distribution of atmospheric polycyclic aromatic hydrocarbons (PAHs) in rural and urban areas of the North Chinese Plain

Passive air sampling (PAS) was employed to study the occurrence of gaseous and particle-bound PAHs in the North Chinese Plain. The averaged concentrations of gaseous and particle-bound PAHs were 485 ± 209 ng/m³ and 267 ± 161 ng/m³, respectively. The PAHs concentrations at urban sites were generally higher than those at rural ones with ratios <1.5 in spring, summer and fall, but differences between them were not significant for the wintertime and annually averaged concentrations. This urban-rural distribution pattern was related to the PAHs emission sources. PAHs spatial variation can be partially (49%) explained by emission with a simple linear regression method. Both the gaseous and particle-bound PAHs were highest in winter and lowest in summer, with winter/summer ratios of 1.8 and 8, respectively. Emission strength was the most important factor for the seasonality.     

Dynamics of soil organic matter in primary and secondary forest succession on sandy soils in The Netherlands: An application of the ROMUL model

We applied the simulation model ROMUL of soil organic matter dynamics in order to analyse and predict forest soil organic matter (SOM) changes following stand growth and also to identify gaps of data and modelling problems. SOM build-up was analysed (a) from bare sand to forest soil during a primary succession in Scots pine forest and (b) on mature forest soil under Douglas fir plantations as an example of secondary succession in The Netherlands. As some of the experimental data were unreliable we compiled a set of various scenarios with different soil moisture regime, initial SOM pools and amount and quality of above and below ground litter input. This allowed us to find the scenarios that reflect the SOM dynamics more realistically. In the Scots pine forest, total litter input was estimated as 0.50 kg m⁻² year⁻¹. Two scenarios were defined for the test runs: (a) forest floor moisture regimes—‘dry, mesic and hydric’ and (b) augmenting a root litter pool with three ratios of needles and branches to roots: 1:1, 1:1.5 and 1:2.0. The scenario finally compiled had the following characteristics: (a) climate for dry site with summer drought and high winter moisture of forest floor; (b) a litter input of 0.25 kg m⁻² year⁻¹ above ground and 0.50 kg m⁻² year⁻¹ below ground; (c) a low nitrogen and ash content in all litter fall fractions. The test runs for the estimation of the initial SOM pools and the amount and proportion of above and below ground litter fall were also performed in the Douglas fir plantation. The inputs of above ground litter tested in various combinations were 0.30 and 0.60 kg m⁻² year⁻¹, and below ground litter 0.30, 0.60 and 0.90 kg m⁻² year⁻¹. The scenario that fitted the experimental data had an SOM pool of 20–25 kg m⁻², an aboveground litter input of 0.6 kg m⁻² year⁻¹and a below ground litter input of 0.9 kg m⁻² year⁻¹. The long-term simulation corresponded well with the observed patterns of soil organic matter accumulation associated with the forest soil development in primary and secondary succession. During primary succession in Scots pine forest on dry sand there is a consistent accumulation of a raw humus forest floor. The soil dynamics in the Douglas fir plantation also coincide with the observed patterns of SOM changes during the secondary succession, with SOM decreasing significantly under young forest, and SOM being restored in the older stands.     

植物区系学中特有现象的研究进展(Ⅱ)--中国植物区系、研究方法与任务

植物区系学中的特有现象是其研究的重点内容,对特有现象的概念、类型、起源及其研究意义作了详细的论述;其次,总结了中国植物区系的基本情况、生活型、分布区学、水平分布、垂直分布与起源等研究成果;第三,介绍了研究特有现象的形态-地理学方法、细胞地理学方法和等位酶分析方法;第四,指出了中国植物区系中特有现象研究所面临的困境,认为应该集中力量重点研究滇黔桂、横断山脉以及华中等3个地区的植物区系基本特征、组成、起源、演化与扩散等内容.     

Reverse logistics in the Chinese auto-parts firms: implementation framework development through multiple case studies

In recent years, the implementation of reverse logistics (RL) in automobile manufacturing has drawn extensive interest from the Chinese government that has launched relevant take-back and environmental regulations and pilot projects. This study focuses on RL implementation issues in Chinese auto-parts manufacturing sector. Based on comprehensive literature review and multiple case studies in the Chinese auto-parts firms, this paper proposes a conceptual RL framework and highlights the major implementation issues. The purpose of this paper is to draw an overall picture of RL in the Chinese context, highlighting implementation issues in the auto-parts sector. Our study suggests that Chinese RL implementation requires close government monitoring mechanism and incentives, top management commitment within firms, technology and human capabilities support.     

苯并芘和邻苯二甲酸二(2-乙基己基)酯复合暴露对稀有鮈鲫的内分泌干扰效应研究

为评价典型有机污染物共存情况下对长江上游三峡库区特有珍稀鱼类的环境风险,以稀有鮈鲫(Gobiocypris rarus)为对象,研究了多环芳烃和酞酸酯的代表物苯并芘(BaP)和邻苯二甲酸二(2-乙基己基)酯(DEHP)单独及复合暴露而引起的内分泌干扰效应。将成年稀有鮈鲫暴露于不同浓度的BaP(0.1、1μg·L~(-1))、DEHP(10、100μg·L~(-1))和BaP+DEHP((0.1+10)、(1+100)μg·L~(-1))中28 d后,测定了体质系数、性激素含量、下丘脑-垂体-性腺(HPG)轴和肝脏中与生殖相关基因的表达。结果表明:在雌鱼中,单独BaP或DEHP暴露对鱼体性激素含量、基因表达量无显著影响; BaP和DEHP复合暴露时,高浓度组睾酮(T)含量升高但雌二醇(E2)含量下降,fsh、vtg、 cyp17和17β-hsd基因表达量上调,cyp19a则下调,表现出协同毒性。因而雌鱼中性激素水平的改变可能与类固醇合成相关基因表达量的改变有关。而无论在单独暴露或是复合暴露情况下,对雄鱼体内性激素无显著影响。以上检测到的激素或基因表达量的改变主要发生在高剂量的复合暴露组中,而长江上游三峡库区水体中多环芳烃和酞酸酯含量低于暴露的最低浓度,因此推测,库区存在的多环芳烃和酞酸酯同时作用时不会对三峡库区上游水体中的鱼类产生生殖内分泌干扰效应。     

Dissolved organic sulfur in streams draining forested catchments in southern China

Dissolved organic sulfur (DOS) is an important fraction for sulfur mobilization in ecosystem. In this work stream waters were sampled in 25 forested sites in southern China to study the dissolved sulfur fractions. Dissolved sulfur was fractionated into dissolved organic sulfur (DOS) and inorganic sulfate (SO₄^2-) for 95 stream water samples. The results showed that the concentration of DOS ranged from 0 to 13.1 mg/L (average 1.3 mg/L) in all the streams. High concentrations of DOS in stream waters were found in the sites with high concentrations of sulfate. DOS constituted less than 60.1% of dissolved sulfur (average 17.9%). Statistical analysis showed that DOS concentration was correlated with SO₄^2- in streams waters and total sulfur in surface layer soils. The results also showed that DOS concentration in stream waters had a seasonal variation, but no trends were found with it. The implication was that the long term sulfur deposition had led the increase of the concentration and fraction of DOS in stream waters in acid rain prevailing regions     

中国黄土释光测年与应用:过去、现在与未来

中国黄土释光测年走过了近40年的历程,这对于推动释光测年技术的进步和黄土古气候古环境研究的深入有重要意义。本文针对中国黄土(以黄土高原为主),简要回顾了以多片法混合矿物热释光(TL)和红外释光(IRSL)测年技术为主的早期(20世纪70年代末期至21世纪第一个十年初期)探索阶段的发展,重点阐述了以成熟的单片再生剂量(SAR)法石英光释光(OSL)技术和延长测年年限的多种方法并存为特征的目前(21世纪以来)研究进展。同时,系统论述了释光测年技术在晚更新世以来中国黄土古气候古环境研究中的意义,如在年代标尺建立、粉尘堆积速率变化重建、地层对比与划分、轨道和亚轨道尺度气候演变与气候事件揭示等方面的应用。另外,也对将来中国黄土释光测年技术的发展方向和其在古气候古环境研究领域的应用前景进行了简单展望。     

Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb^3＋-tiron complex as a fluorescence probe

A sensitive method based on the fluorescence quenching effect of the Tb^3＋-Tiron complex is proposed for the determination of alkali-labile phosphoprotein phosphorus （ALP） released from fish plasma. The detection limit was 5.4 ng/ml （S/N=2）, and the relative standard deviation of the quenching effect （6 replicates） was 4.6%. The results obtained by the proposed method were in good agreement with those obtained by the colorimetric assay. The advantages of the present method are its relatively simple detection procedure, the lack of toxic organic solvents, and high sensitivity.     

中华鳖出血性肠炎的药物防治研究

对由实验定筛选的中华鳖出血肠炎病病原菌的最佳防治药物为本正交试验的第6组,即A2B3C1,其抑菌圈达21.5mm.临床应用中采用中西结合复方制剂,即用第6组试验药物再加上中药白头翁汤(加减),其疗效更佳.